基于壳层隔绝纳米粒子和在线裂解-吹扫捕集的血液氰化物表面增强拉曼光谱快速检测方法

引用本文: 朱颖洁, 郭磊, 刘易, 龚莹, 邱泽武, 吴剑峰, 谢剑炜. 基于壳层隔绝纳米粒子和在线裂解-吹扫捕集的血液氰化物表面增强拉曼光谱快速检测方法[J]. 分析化学, 2017, 45(5): 627-632. doi: 10.11895/j.issn.0253-3820.160902 shu

Citation: ZHU Ying-Jie, GUO Lei, LIU Yi, GONG Ying, QIU Ze-Wu, WU Jian-Feng, XIE Jian-Wei. Rapid Surface-Enhanced Raman Spectroscopic Detection of Blood Cyanide Based on Pinhole Shell-Isolated Nanoparticle and Online Lysis-Purging and Trapping Approach[J]. Chinese Journal of Analytical Chemistry, 2017, 45(5): 627-632. doi: 10.11895/j.issn.0253-3820.160902 shu

基于壳层隔绝纳米粒子和在线裂解-吹扫捕集的血液氰化物表面增强拉曼光谱快速检测方法

朱颖洁 ^1, ,
郭磊 ^1, ,
刘易 ^1, ,
龚莹 ^1, ,
邱泽武 ^2, ,
吴剑峰 ^1, ,
谢剑炜 ^1,

1.
军事医学科学院毒物药物研究所, 抗毒药物与毒理学国家重点实验室, 北京 100850;
2.
军事医学科学院附属医院中毒救治科, 北京 100071
基金项目:
本文系国家重大科学仪器设备开发专项（No. 2011YQ03012411）和重大新药创新国家科技重大专项"中药新药安全性检测技术与标准研究"项目（No. 2014ZX09304307001）资助

摘要: 建立了一种快速、简便的基于壳层隔绝纳米粒子和在线裂解捕集技术的血液氰化物表面增强拉曼光谱（SERS）检测方法。构建了新型在线裂解-吹扫捕集装置，通过浓H₂SO₄酸化，将全血中结合态氰化物裂解，并转化为挥发性的HCN气体，经N₂吹扫至碱液中形成NaCN，实现了中毒血液中氰化物的高效裂解与捕集；在强碱性条件下，以壳层隔绝金纳米粒子为基底，极大地削弱了氰化物的溶金效应，使得检测信号稳定，存在时间由普通金纳米粒子的5 min提高至1 h；方法检出限达10 μg/L，线性范围为100~2000 μg/L。将此方法应用于氰化物染毒大鼠及真实氰化物中毒患者全血的快速检测，为氰化物中毒快速检测提供了一种灵敏、特异、可靠的新方法。

关键词:

English

Rapid Surface-Enhanced Raman Spectroscopic Detection of Blood Cyanide Based on Pinhole Shell-Isolated Nanoparticle and Online Lysis-Purging and Trapping Approach

1.
Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, and State Key Laboratory of Toxicology and Medical Countermeasures, Beijing 100850, China;
2.
Department of Poisoning Treatment, Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071, China
Received Date: 15 December 2016
Revised Date: 23 February 2017
Fund Project:
This work was supported by the National Major Instrument Project of the Ministry of Science and Technology of China (No. 2011YQ03012411) and the National Science and Technology Major Project (No. 2014ZX09304307001).

Abstract: A rapid and simple method for the determination of cyanide in blood was developed based on pinhole shell-isolated nanoparticles (pinSHINs)-enhanced Raman spectroscopy and an online lysis-purging and trapping approach. In the online lysis-purging and trapping device, the bound cyanide in blood can be cleaved through sulfuric acid acidification, and transferred into HCN volatile gas, then purged into alkaline solution to form NaCN solution, thus high-efficient liberation and entrapment of cyanide from the methemoglobin-bound form can be achieved. The pinSHINs substrate is quite stable to weaken the gold-dissolution effect caused by cyanide under strong alkaline condition, and therefore the detection window can be prolonged to 1 h comparing with 5 min of AuNPs. A limit of detection down to 10 μg/L and a linear range from 100-2000 μg/L in blood were achieved in this method. This method was further applied to rapid measurement of blood samples of cyanide exposed rats and clinic poisoned patients, which provided a sensitive, selective and reliable way for rapid detection of cyanide poisoning.

Key words:

1. [1]
  1 Barillo D J, Goode R, Esch V. J. Burn Care Rehabil., 1994, 15(1):46-57
2. [2]
  Baud F J, Barriot P, Toffis V, Riou B, Vicaut E, Lecarpentier Y, Bourdon R, Astier A, Bismuth C. N. Engl. J. Med., 1991, 325(25):1761-1766
3. [3]
  Gleadow R M. Plant Biol., 2014, 65(65):155-185
4. [4]
  Conn E E. Effects of Poisonous Plants on Livestock. New York:Academic Press, 1978:301-310
5. [5]
  Joint F A O. World Health Organization, WHO Expert Committee on Food Additives. Safety Evaluation of Certain Food Additives and Contaminants:Prepared by the Seventy Fourth Meeting of the Joint FAO[R/OL]. 2012:299[201-11-19]
6. [6]
  Chaudhary M T, Sarwar M, Tahir A M, Tahir M A, Mustafa G, Wattoo S A, Imran M, Subhani A. Australian J. Forensic Sci., 2015, 48(1):42-49
7. [7]
  XU Hong, SONG Yin-Sheng, CAO Wen-Ting, ZHANG Chun-Ling. Chin J Health Lab Tec, 2015, 25(11):1705-1707 徐虹, 宋寅生, 曹文婷, 张春玲. 中国卫生检验杂志, 2015, 25(11):1705-1707
8. [8]
  Lundquist P, Sörbo B. Clin. Chem., 1989, 35(4):617-619
9. [9]
  Calafat A M. Stanfill S B. J. Chromatogr. B, 2002, 772(1):131-137
10. [10]
  Chinaka S, Takayama N, Michigami Y, Ueda K. J. Chromatogr. B, 1998, 713(2):353-359
11. [11]
  Lacroix C, Saussereau E, Boulanger F, Goullé J P. J. Anal. Toxicol., 2011, 35(3):143-147
12. [12]
  Zhu Q X, Cao Y B, Cao Y Y, Chai Y F, Lu F. Anal. Bioanal.Chem., 2014, 406(7):1877-1884
13. [13]
  LUO Zhi-Xun, FANG Yan. Spectroscopy and Spectral Analysis, 2006, 26(2):358-364 骆智训, 方炎. 光谱学与光谱分析, 2006, 26(2):358-364
14. [14]
  LIU Jiang-Mei, LIU Wen-Han, TENG Yuan-Jie, YUAN Rong-Hui. Chin. J. Lumin, 2015, 36(12):1477-1484 刘江美, 刘文涵, 滕渊洁, 袁荣辉. 发光学报, 2015, 36(12):1477-1484
15. [15]
  ZUO Qi, CHEN Yao, SHI Cai-Xia, CHEN Zeng-Ping. Chinese J. Anal. Chem., 2015, 43(11):1656-1663 左奇, 陈瑶, 石彩霞, 陈增萍. 分析化学, 2015, 43(11):1656-1663
16. [16]
  Shi Y E, Wang W S, Zhan J H. Nano Res., 2016, 9(8):2487-2497
17. [17]
  GAO Jing, WU Jian-Feng, GAO Hai-Yue, GUO Lei, XIE Jian-Wei. Chinese J. Anal. Chem., 2014, 42(10):1465-1470 高敬, 吴剑峰, 高海月, 郭磊, 谢剑炜. 分析化学, 2014, 42(10):1465-1470
18. [18]
  Gao H Y, Wu J F, Zhu Y J, Guo L, Xie J W. J. Raman. Spectrosc., 2015, 47(2):233-239
19. [19]
  Zhu Y J, Wu J F, Gao H Y, Liu G K, Tian Z Q, Feng J L, Guo L, Xie J W. RSC Adv., 2016, 6(65):59919-59926
20. [20]
  Li J F, Huang Y F, Ding Y, Yang Z L, Li S B, Zhou X S, Fan F R, Zhang W, Zhou Z Y, Ren B. Nature, 2010, 464(7287):392-395
21. [21]
  Gao J, Guo L, Wu J F, Feng J L, Wang S M, Lai F L, Xie J W, Tian Z Q. J. Raman. Spectrosc., 2014, 45(8):619-626
22. [22]
  Lundquist P, Rosling H, Sörbo B. Clin. Chem., 1985, 31(4):591-595
23. [23]
  Hughes C, Lehner F, Dirikolu L, Harkins D, Boyles J, McDowell K, Tobin T, Crutchfield J, Sebastian M, Harrison L. Toxicol. Mechanisms Method, 2008, 13(2):129-138
24. [24]
  Frens G. Nature, 1973, 241(105):20-22
25. [25]
  Li J F, Tian X D, Li S B, Anema J R, Yang Z L, Ding Y, Wu Y F, Zeng Y M, Chen Q Z, Ren B. Nat. Protoc., 2013, 8(1):52-65
26. [26]
  Sousa A B, Manzano H, Sotoblanco B, Górniak S L. Arch. Toxicol., 2003, 77(6):330-334
27. [27]
  Haiss W, Thanh N T K, Aveyard J, Fernig D G. Anal. Chem., 2007, 79(11):4215-4221
28. [28]
  Senapati D, Dasary S S R, Signh A K, Senapati T, Yu H, Ray P C. Chem. Eur J., 2001, 17(30):8445-8451
29. [29]
  Vesey C J, Wilson J. J. Pharm. Pharmac., 1978, 30(1):20-26

扫一扫看文章

计量

PDF下载量: 3
文章访问数: 738
HTML全文浏览量: 10

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

基于壳层隔绝纳米粒子和在线裂解-吹扫捕集的血液氰化物表面增强拉曼光谱快速检测方法

English

Rapid Surface-Enhanced Raman Spectroscopic Detection of Blood Cyanide Based on Pinhole Shell-Isolated Nanoparticle and Online Lysis-Purging and Trapping Approach

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于壳层隔绝纳米粒子和在线裂解-吹扫捕集的血液氰化物表面增强拉曼光谱快速检测方法

English

Rapid Surface-Enhanced Raman Spectroscopic Detection of Blood Cyanide Based on Pinhole Shell-Isolated Nanoparticle and Online Lysis-Purging and Trapping Approach

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

文章相关

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs