超高效液相色谱-四极杆飞行时间质谱法非靶向快速筛查进口粮谷中未知的农药残留

引用本文: 周秀锦, 陈宇, 杨赛军, 邵宏宏, 沈飚, 詹舜安. 超高效液相色谱-四极杆飞行时间质谱法非靶向快速筛查进口粮谷中未知的农药残留[J]. 色谱, 2017, 35(8): 787-793. doi: 10.3724/SP.J.1123.2017.03024 shu

Citation: ZHOU Xiujin, CHEN Yu, YANG Saijun, SHAO Honghong, SHEN Biao, ZHAN Shun'an. Rapid screening of pesticide residues in imported grains by ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry[J]. Chinese Journal of Chromatography, 2017, 35(8): 787-793. doi: 10.3724/SP.J.1123.2017.03024 shu

超高效液相色谱-四极杆飞行时间质谱法非靶向快速筛查进口粮谷中未知的农药残留

周秀锦 ^1, ,
陈宇 ^1, ,
杨赛军 ^1, ,
邵宏宏 ^1, ,
沈飚 ^1, ,
詹舜安 ^2,

1.
舟山出入境检验检疫局, 浙江舟山 316021;
2.
安捷伦科技中国有限公司, 北京 100102
基金项目:
国家质检总局科技计划项目（2015IK306）.

摘要: 利用快速提取农药（fast pesticide extraction，FaPEx）方法前处理样品，并结合超高效液相色谱-四极杆飞行时间质谱（UPLC-Q-TOF）技术建立了非靶向快速筛查进口粮谷中未知的多种农药残留方法。采用1%（v/v）醋酸乙腈溶液提取粮谷中未知的农药残留，FaPEx固相萃取柱净化、浓缩，UPLC-Q-TOF检测。利用目标化合物特征离子的精确质量数、同位素匹配、二级碎片信息和保留时间进行数据库匹配，筛查可疑未知农药。结果表明，该方法无需对照标准品即可快速筛查进口粮谷中的农药残留，能够应用于进口粮谷样品的实际筛查。该方法快速、准确、分析通量高，可以为进口粮谷中农药残留的快速筛查和质量控制提供重要的方法依据。

关键词:

English

Rapid screening of pesticide residues in imported grains by ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry

1.
Zhoushan Entry-Exit Inspection and Quarantine Bureau, Zhoushan 316021, China;
2.
Agilent Technology China Ltd, Beijing 100102, China
Received Date: 21 March 2017
Fund Project:
Science and Technology Plan Project of General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China (No. 2015IK306).

Abstract: A fast sample pretreatment method by fast pesticide extraction (FaPEx) method combined with ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry was developed for screening of unknown pesticide residues in imported grains. The samples were extracted with 1% (v/v) acetic acid acetonitrile solution, cleaned-up by solid phase extraction column with FaPEx cartridges, and determined by UPLC-Q-TOF. The data was compared with the library with the accurate mass, isotopic distribution, fragmentation information and retention time. The results showed that this method can be used for the rapid screening of pesticide residues in imported grains without the reference standards, and it was applied to the actual screening of imported grain samples. The method is high efficiency, sensitivity and accuracy, which can meet the requirement for the rapid screening of pesticide residues in imported grains.

Key words:

fast pesticide extraction (FaPEx) method
/ ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry (UPLC-Q-TOF)
/ rapid screening
/ pesticide residues
/ grains

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

超高效液相色谱-四极杆飞行时间质谱法非靶向快速筛查进口粮谷中未知的农药残留

English

Rapid screening of pesticide residues in imported grains by ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry

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中国化学会秘书处

超高效液相色谱-四极杆飞行时间质谱法非靶向快速筛查进口粮谷中未知的农药残留

English

Rapid screening of pesticide residues in imported grains by ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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