银纳米簇水凝胶对油田管道中硫酸盐还原菌的抗菌性能研究

引用本文: 杨梅, 邵泽宇, 廖晓玲, 徐文峰, 张园园. 银纳米簇水凝胶对油田管道中硫酸盐还原菌的抗菌性能研究[J]. 分析化学, 2020, 48(12): 1674-1680. doi: 10.19756/j.issn.0253-3820.191705 shu

Citation: YANG Mei, SHAO Ze-Yu, LIAO Xiao-Ling, XU Wen-Feng, ZHANG Yuan-Yuan. Antibacterial Activity of Ag Nanoclusters Hydrogel to Sulfate Reducing Bacteria in Oil Field Pipeline[J]. Chinese Journal of Analytical Chemistry, 2020, 48(12): 1674-1680. doi: 10.19756/j.issn.0253-3820.191705 shu

银纳米簇水凝胶对油田管道中硫酸盐还原菌的抗菌性能研究

1.
重庆科技学院生物医学工程研究院, 重庆 401331;
2.
纳微生物医学检测技术重庆市工程实验室, 重庆 401331
基金项目:
本文系国家自然科学基金项目（No.11532004）、重庆市科委自然科学基金项目（Nos.CSTC2018JCYJAX0286，CSTC2019JCYJ-msxmX0221，CSTC2019JSCX-msxmX0231）和重庆科技学院研究生科技创新计划项目（No.YKJCX1820206）资助

摘要: 以AgNO₃和L-半胱氨酸为原料，利用紫外光照还原法制备了具有抗菌效应的银纳米簇水凝胶新型抗菌材料，利用透射电子显微镜（TEM）、X射线光电子能谱仪（XPS）、扫描电子显微镜（SEM）和能谱仪（EDS）对材料结构和形貌进行表征；利用厌氧工作站，通过最小抑菌浓度（MIC）、最小杀菌浓度（MBC）实验、生长曲线以及细菌染色实验，深入研究了银纳米簇水凝胶对硫酸盐还原菌（SRB）的抗菌性能。结果表明，与常规无机抗菌材料NaClO相比，此银纳米簇水凝胶对SRB具有优良的抗菌作用，对SRB的最小抑菌浓度为18.75 μg/mL，最小杀菌浓度为37.5 μg/mL。

关键词:

English

Antibacterial Activity of Ag Nanoclusters Hydrogel to Sulfate Reducing Bacteria in Oil Field Pipeline

1.
Institute of Biomedical Engineering, Chongqing University of Science and Technology, Chongqing 401331, China;
2.
Chongqing Engineering Laboratory of Nano/Micro Biological Medicine Detection Technology, Chongqing University of Science and Technology, Chongqing 401331, China
Received Date: 21 December 2019
Revised Date: 28 October 2020
Fund Project:
This work was supported by the National Natural Science Foundation of China (No. 11532004), the Natural Science Foundation of Chongqing Science and Technology Commission (Nos. CSTC2018jcyjAX0286, CSTC2019JCYJ-msxmX0221, CSTC 2019JSCX-msxmX0231), and the Postgraduate Science and Technology Innovation Program of Chongqing University of Science and Technology (No. YKJCX1820206).

Abstract: A novel antibacterial material of Ag nanoclusters hydrogel using silver nitrate and L-cysteine as raw materials was prepared by ultraviolet light reduction method. The structure and morphology of the material were characterized by transmission electron microscope (TEM), X-ray photoelectron spectra (XPS), scanning electron microscope (SEM) and X ray energy spectrum analysis (EDS). The antimicrobial properties of Ag nanoclusters hydrogel against sulfate reducing bacteria (SRB) were further studied through the minimum inhibitory concentration (MIC), the minimum bactericidal concentration (MBC), growth curve and bacterial staining experiment using anaerobic workstations. The results indicated that, compared with the conventional inorganic antibacterial material sodium hypochlorite, the prepared Ag nanoclusters hydrogel had excellent antibacterial effect on SRB, and the MIC and MBC against SRB were 18.75 μg/mL and 37.5 μg/mL, respectively.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

银纳米簇水凝胶对油田管道中硫酸盐还原菌的抗菌性能研究

English

Antibacterial Activity of Ag Nanoclusters Hydrogel to Sulfate Reducing Bacteria in Oil Field Pipeline

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

银纳米簇水凝胶对油田管道中硫酸盐还原菌的抗菌性能研究

English

Antibacterial Activity of Ag Nanoclusters Hydrogel to Sulfate Reducing Bacteria in Oil Field Pipeline

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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