气相色谱-氮磷检测器法表征家蝇对马拉硫磷的水解代谢

引用本文: 张怡, 史雪岩, 高希武. 气相色谱-氮磷检测器法表征家蝇对马拉硫磷的水解代谢[J]. 色谱, 2019, 37(4): 449-453. doi: 10.3724/SP.J.1123.2018.10010 shu

Citation: ZHANG Yi, SHI Xueyan, GAO Xiwu. Characterization of hydrolytic metabolism of malathion with Musca domestica by gas chromatography with nitrogen-phosphorus detector[J]. Chinese Journal of Chromatography, 2019, 37(4): 449-453. doi: 10.3724/SP.J.1123.2018.10010 shu

气相色谱-氮磷检测器法表征家蝇对马拉硫磷的水解代谢

中国农业大学植物保护学院, 北京 100193
基金项目:
国家重点研发计划项目（2018YFD0200408）.

摘要: 为明确家蝇对马拉硫磷的水解代谢能力及相关代谢抗性，在用常规模式底物α-萘乙酯表征了敏感及抗性家蝇的水解酶活性的基础上，使用气相色谱-氮磷检测器（GC-NPD）法比较了抗性及敏感家蝇水解酶对马拉硫磷的水解代谢能力的差异。结果表明，用2：1（v/v）的乙酸乙酯与正己烷混合物萃取后，GC-NPD可成功测定家蝇酶制备液与马拉硫磷温育后的马拉硫磷含量变化，从而可以表征家蝇酶制备液对马拉硫磷的代谢能力。马拉硫磷抗性家蝇水解酶代谢α-萘乙酯的能力是敏感家蝇的1.39倍，代谢马拉硫磷的能力是敏感家蝇的6.68倍，表明家蝇对马拉硫磷的抗性与其对马拉硫磷代谢能力增强有关。该法可用于家蝇等昆虫对马拉硫磷的代谢能力及相关抗性机制研究。

关键词:

English

Characterization of hydrolytic metabolism of malathion with Musca domestica by gas chromatography with nitrogen-phosphorus detector

College of Plant Protection, China Agricultural University, Beijing 100193, China
Received Date: 10 October 2018
Fund Project:
National Key Research and Development Program of China (No. 2018YFD0200408).

Abstract: To examine the hydrolytic metabolism and related resistance of housefly Musca domestica (M. domestica) to malathion, differences in the hydrolytic metabolic abilities between malathion-susceptible and malathion-resistant M. domestica were examined using gas chromatography with nitrogen-phosphorus detector (GC-NPD). After incubation of M. domestica abdomen homogenates with malathion, the malathion residue was quantitatively determined by GC-NPD after extraction with a mixture of ethyl acetate and n-hexane at 2:1 (v/v). The hydrolytic metabolic abilities of M. domestica to malathion were then characterized. By comparing the differences in hydrolytic abilities between malathion-susceptible and malathion-resistant M. domestica, it was found that the hydrolytic abilities of malathion-resistant M. domestica were 6.68-fold than that of malathion-susceptible M. domestica, while the hydrolytic abilities toward α-naphthyl acetate were 1.39-fold. Resistance to malathion is related to the enhanced hydrolytic metabolism of malathion by M. domestica. Further understanding of the metabolic resistance mechanism will assist in developing a management strategy for malathion-resistant M. domestica. This method could be used for studying the metabolic ability and related resistance of M. domestica toward malathion.

Key words:

gas chromatography with nitrogen-phosphorus detector (GC-NPD)
/ malathion
/ metabolic resistance
/ Musca domestica

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气相色谱-氮磷检测器法表征家蝇对马拉硫磷的水解代谢

English

Characterization of hydrolytic metabolism of malathion with Musca domestica by gas chromatography with nitrogen-phosphorus detector

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中国化学会秘书处

气相色谱-氮磷检测器法表征家蝇对马拉硫磷的水解代谢

English

Characterization of hydrolytic metabolism of malathion with Musca domestica by gas chromatography with nitrogen-phosphorus detector

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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