引用本文:
Met FANG, Li SHENG, Hut Wan HAN, Xiao YU, Rut ZHAO, Guo Quan LIU. A Novel Method for Studying the interaction of Macromolecule with Small Molecule by Means of Affinity Capillary Electrophoresis[J]. Chinese Chemical Letters,
1999, 10(3): 235-238.
Citation: Met FANG, Li SHENG, Hut Wan HAN, Xiao YU, Rut ZHAO, Guo Quan LIU. A Novel Method for Studying the interaction of Macromolecule with Small Molecule by Means of Affinity Capillary Electrophoresis[J]. Chinese Chemical Letters, 1999, 10(3): 235-238.
Citation: Met FANG, Li SHENG, Hut Wan HAN, Xiao YU, Rut ZHAO, Guo Quan LIU. A Novel Method for Studying the interaction of Macromolecule with Small Molecule by Means of Affinity Capillary Electrophoresis[J]. Chinese Chemical Letters, 1999, 10(3): 235-238.
A Novel Method for Studying the interaction of Macromolecule with Small Molecule by Means of Affinity Capillary Electrophoresis
摘要:
A novel capillary electrophoresis method coupled with on-line microdialysis using an attachable electrode has been developed to study the interaction of macromolecule with small molecule.The binding constants of bovine serum album (BSA) with D.L-tryptophan (Trp), sulfamethoxazole (SMZ) with trypsin and chymotrypsin were determined.These values are 2.3×104L/mol for BSA-L-Trp.1.77×103 L/mol for BSA-D-TrP in PH 7.4, 50 mmol/ L phosphate; 1.4×104 L/mol for SMZ-trypsin and 6.0×103 L/mol for SMZ-chymotrypsin in PH 6.5, 25 mmol/L Tris buffer.The proposed method has merits of speed.low sample consumption and readily available to be performed in desired physiological conditions.
English
A Novel Method for Studying the interaction of Macromolecule with Small Molecule by Means of Affinity Capillary Electrophoresis
Abstract:
A novel capillary electrophoresis method coupled with on-line microdialysis using an attachable electrode has been developed to study the interaction of macromolecule with small molecule.The binding constants of bovine serum album (BSA) with D.L-tryptophan (Trp), sulfamethoxazole (SMZ) with trypsin and chymotrypsin were determined.These values are 2.3×104L/mol for BSA-L-Trp.1.77×103 L/mol for BSA-D-TrP in PH 7.4, 50 mmol/ L phosphate; 1.4×104 L/mol for SMZ-trypsin and 6.0×103 L/mol for SMZ-chymotrypsin in PH 6.5, 25 mmol/L Tris buffer.The proposed method has merits of speed.low sample consumption and readily available to be performed in desired physiological conditions.
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