光谱法研究哈巴俄苷与人血清白蛋白的结合反应

引用本文: 曹团武, 周坤, 黄文兵, 时建伟, 谭晓平, 黄春林, 冉嫒. 光谱法研究哈巴俄苷与人血清白蛋白的结合反应[J]. 分析化学, 2017, 45(5): 700-706. doi: 10.11895/j.issn.0253-3820.160873 shu

Citation: CAO Tuan-Wu, ZHOU Kun, HUANG Wen-Bing, SHI Jian-Wei, TAN Xiao-Ping, HUANG Chun-Lin, RAN Ai. Spectroscopic Study of Interaction of Harpagoside and Human Serum Albumin[J]. Chinese Journal of Analytical Chemistry, 2017, 45(5): 700-706. doi: 10.11895/j.issn.0253-3820.160873 shu

光谱法研究哈巴俄苷与人血清白蛋白的结合反应

长江师范学院化学化工学院, 武陵山天然药物研究与开发实验室, 重庆 408100
基金项目:
本文系重庆市教委科学技术研究项目（No.KJ15012010）；长江师范学院科研创新平台建设项目（No.2015XJPT01）资助。

摘要: 在不同温度及模拟血液pH值条件下，采用荧光光谱法和紫外-可见吸收光谱法研究了哈巴俄苷（Harpagoside，HAR）与人血清白蛋白（Human serum albumin，HSA）的结合反应。结果表明，HAR有规律地使HSA内源荧光猝灭，猝灭常数随温度升高而降低，其猝灭机制为两者形成复合物而引起的的静态猝灭；不同条件下两者结合常数K_A均大于10⁵ L/mol，结合位点数n≈1。由Van't Hoff方程计算获得了不同条件下HAR与HSA相互作用的热力学参数，由ΔG、ΔH和ΔS均小于0可知，两者结合的主要作用力是氢键和范德华力，且两者结合是吉布斯自由能降低的自发过程。根据Frster非辐射转移理论计，计算了不同条件下HAR与HSA的结合距离r在4.01-4.28 nm范围内，表明两者结合过程发生了非辐射能量转移。同步荧光光谱表征结果表明，HAR使HSA的色氨酸和酪氨酸残基所处的微环境极性增强，疏水性减弱，导致HSA构象发生了一定程度的改变。

关键词:

English

Spectroscopic Study of Interaction of Harpagoside and Human Serum Albumin

Laboratory of Natural Medicine Research and Development in Wuling Mountain, School of Chemistry and Chemical Engineering, Yangtze Normal University, Chongqing 408100, China
Received Date: 30 November 2016
Revised Date: 08 March 2017
Fund Project:
This work was supported by the Science and Technology Project of Chongqing Municipal Education Commission (No.KJ15012010), and the Construction of Scientific Research Platform of Yangtze Normal University (No.2015XJPT01).

Abstract: Harpagoside (HAR) is believed to be a main compound in Scrophularia ningpoensis which possess a broad of biological activities. Human serum albumin (HSA) has important physiological roles in transportation, distribution and metabolism of many endogenous and exogenous substances in body. It is great significance in pharmacology to investigate the interaction mechanism of HAR and HSA. In this work, the interaction between HAR and HSA was investigated by fluorescence and ultraviolet absorption spectroscopy at different pH (pH=4.0, 7.4, and 9.0) and temperatures (297, 310 and 323 K). The experimental results showed that the HAR could cause the fluorescence quenching of HSA through a static quenching procedure, showing that the HAR regularly quenched the intrinsic fluorescence of HSA, and a decrease in the quenching constant was observed with an increase in temperature. Under different conditions, all the magnitude of binding constants (K_A) was larger than 10⁵ L/mol and the number of binding sites (n) in the binary system were approximate to 1. Base on the magnitude of enthalpy and entropy changes, the negative values of ΔG, ΔH and ΔS revealed that the binding of HAR with HSA was spontaneous and exothermic process, and the main interaction forces of the HAR with HAR were van der Waals forces and/or hydrogen bonding interaction. The binding distance (r) between the HAR and HSA was calculated to be about 4.2 nm based on the theory of Frster's nonradiation energy transfer, which indicated that the energy transfer from HSA to HAR occurred with high possibility. What was more, the synchronous florescence spectroscopy confirmed the conformational changes of HSA during the binding reaction.

Key words:

1. [1]
  1 ZHU Rui-Rui, NI Yong-Nian. Chem. Res. Appl., 2011, 23(10):1281-1289 朱瑞瑞, 倪永年. 化学研究与应用, 2011, 23(10):1281-1289
2. [2]
  WU Yu-Hang, HAN Zhong-Bao, MA Jia-Ze, HE Yan, LIU Li-Yan, XIN Shi-Gang, YU Zhan. Spectrosc. Spect. Anal., 2016, 36(3):765-769 吴宇航, 韩忠宝, 马嘉泽, 何妍, 刘丽艳, 辛世刚, 于湛. 光谱学与光谱分析, 2016, 36(3):765-769
3. [3]
  Hossain M, Khan A Y, Suresh Kumar G. Plos One, 2011, 6(4):e18333, 1-12
4. [4]
  Shi J H, Chen J, Wang J, Zhu Y Y, Qiang Q. Spectrochim. Acta A, 2015, 149:630-637
5. [5]
  Rajendiran N, Thulasidhasan J. Spectrochim. Acta A, 2015, 144:183-191
6. [6]
  Chinese Pharmacopoeia Commission. The Chinese Pharmacopoeia, 10th Ed., 2015, Part 1:117 中国药典委员会. 中国药典 (第十版), 2015, 一部:117
7. [7]
  XIE Xiao-Yan, XIA Chun-Sen. Asia-Pac. Tradit. Med., 2010, 6(5):121-124 谢小艳,夏春森. 亚太传统医药, 2010, 6(5):121-124
8. [8]
  ZHANG Liu-Qiang, LI Yi-Ming. Chin. Tradit. Herb. Drugs, 2011, 42(11):2360-2368 张刘强, 李医明. 中草药, 2011, 42(11):2360-2368
9. [9]
  XU Fu-Quan, XU Xu-Dong, CHEN Shi-Lin. Mod. Chin. Med., 2013, 15(9):752-759 许福泉, 许旭东, 陈士林. 中国现代中药, 2013, 15(9):752-759
10. [10]
  XIE Li-Hua, LIU Hong-Yu, QIAN Rui-Qin, ZHANG Chun-Ying, WANG Jian-Hua, CAI Shao-Qing. J. Peking Univ. (Health Sci.), 2001, 33(3):283-284 谢丽华, 刘洪宇, 钱瑞琴, 张春英, 王建华, 蔡少青. 北京大学学报(医学版), 2001, 33(3):283-284
11. [11]
  CUI Zhong-Sheng, DI Ke-Qian, MA Huan-Yun. Shandong Med. J., 2009, 49(25):60-61 崔忠生, 邸科前, 马焕云. 山东医药, 2009, 49(25):60-61
12. [12]
  Sun X Y,Xiong Z K, Zhang Y F, Meng Y, Xu G, Xia Z M, Li J M, Zhang R, Ke Z J, Xia Z Q, Hu Y E. J. Neurochem., 2012, 120:1072-1083
13. [13]
  Schopohl P, Gruneberg P, Melzig M P. Fitoterapia, 2016, 110:157-165
14. [14]
  Wu Q, Wen X D, Qi L W, Wang W, Yi L, Bi Z M, Li P. J. Chromatogr. B, 2009, 877:751-756
15. [15]
  DONG She-Ying, LI Zhi-Qin, HUANG Ting-Lin. Sci. Sin. Chim., 2014, 44(3):373-380 董社英, 李志芹, 黄廷林. 中国科学:化学, 2014, 44(3):373-380
16. [16]
  GUO Qing-Lian, PAN Ling-Li, YANG Li-Yun, HE Huan, ZHANG Ye-Zhong, LIU Yi. Acta Phys. Chim. Sin., 2016, 32(1):274-282 郭清莲, 潘凌立, 杨立云, 何欢, 张业中, 刘义. 物理化学学报, 2016, 32(1):274-282
17. [17]
  Meti M D, Nandibewoor S T, Joshi S D, More U A, Chimatadar S A. J. Iran. Chem. Soc., 2016, 13:1325-1338
18. [18]
  LIU Yuan, LONG Hai, XIE Meng-Xia. Acta Phys. Chim. Sin., 2008, 24(4):715-719 刘媛, 龙海, 谢孟峡. 物理化学学报, 2013, 29(12):2647-2654
19. [19]
  ZHOU Neng, LIANG Yi-Zeng, WANG Ping, LIU Shao, WANG Bing, ZENG Mao-Mao. Chinese J. Anal. Chem., 2008, 36(8):1066-1070 周能, 梁逸曾, 王平, 刘韶, 王兵, 曾茂茂. 分析化学, 2008, 36(8):1066-1070
20. [20]
  Garg A, Mark M D, Gokara M, Malleda C, Suresh R C, Subramanyam R. Plos One, 2013, 8(5):e63805, 1-17
21. [21]
  CAO Tuan-Wu, YANG Ji-Dong. Acta Phys. Chim. Sin., 2008, 24(4):715-719 曹团武, 杨季东. 物理化学学报, 2008, 24(4):715-719
22. [22]
  HE Wen-Ying, YAO Xiao-Jun, HU Zhi-De, CHEN Guang-Ying. Acta Phys. Chim. Sin., 2010, 26(1):221-229 何文英, 姚晓军, 胡之德, 陈光英. 物理化学学报, 2010, 26(1):221-229
23. [23]
  JIANG Tao, MA Liang, ZHANG Yu-Hao, WANG Jia-Man. Chin. J. Anal. Chem., 2016, 44(1):54-60 江涛, 马良, 张宇昊, 王佳曼. 分析化学, 2016, 44(1):54-60
24. [24]
  YANG Shu-Ping, HAN Li-Jun, PAN Yan, WANG Da-Qi, ZHOU Ya-Nan, ZHANG Fan. Sci. Sin. Chim., 2013, 43(7):858-868 杨树平, 韩立军, 潘燕, 王大奇, 周亚男, 张凡. 中国科学:化学, 2013, 43(7):858-868

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光谱法研究哈巴俄苷与人血清白蛋白的结合反应

English

Spectroscopic Study of Interaction of Harpagoside and Human Serum Albumin

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通讯作者: 陈斌, bchen63@163.com

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光谱法研究哈巴俄苷与人血清白蛋白的结合反应

English

Spectroscopic Study of Interaction of Harpagoside and Human Serum Albumin

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

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CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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