同步荧光法监控人乳脂质替代品氧化过程

引用本文: 李雪, 曹君, 白新鹏, 张芳芳. 同步荧光法监控人乳脂质替代品氧化过程[J]. 分析化学, 2018, 46(4): 543-549. doi: 10.11895/j.issn.0253-3820.171230 shu

Citation: LI Xue, CAO Jun, BAI Xin-Peng, ZHANG Fang-Fang. Monitoring of Oxidation Process of Human Milk Lipid Substitutes by Molecular Fluorescence Spectroscopy[J]. Chinese Journal of Analytical Chemistry, 2018, 46(4): 543-549. doi: 10.11895/j.issn.0253-3820.171230 shu

同步荧光法监控人乳脂质替代品氧化过程

海南大学食品学院, 生物活性物质与功能食品开发重点实验室, 海口 570228
基金项目:
本文系国家自然科学基金项目（No.31601579）、海南省重点研发计划项目（No.ZDYF2016093）和海南省教育厅高等学校科学研究项目（No.Hnky2016ZD-1）资助

摘要: 以市售大豆油、玉米油、椰子油、葵花籽油和鱼油为原料油脂，通过气相色谱仪检测其脂肪酸含量，并根据原料油脂的脂肪酸特点和GB 10765-2010的相关规定，通过Matlab软件和Excel线性规划求解，设计出6种人乳脂质替代品样品，采用Schaal烘箱加速氧化法，研究同步荧光技术结合传统化学试剂法（氧化指标：过氧化值、茴香胺值、总氧化值）监控6种样品的氧化过程，通过统计学分析数据，探索荧光强度和氧化指标之间的统计学关系。结果表明：椰子油的主要脂肪酸为月桂酸和肉豆蔻酸，其饱和脂肪酸含量最高（93.75%±0.06%）；而其余油脂主要由棕榈酸、亚油酸等组成，其中鱼油的不饱和脂肪酸最高。随着氧化时间的延长，荧光强度逐渐减弱，氧化指标数值不断增大；相关分析和回归分析结果表明，6种样品的氧化指标与荧光强度的变化均呈负相关，R²均大于0.9。

关键词:

English

Monitoring of Oxidation Process of Human Milk Lipid Substitutes by Molecular Fluorescence Spectroscopy

College of Food Science and Technology, Hainan University, Haikou 570228, China
Received Date: 05 September 2017
Revised Date: 25 January 2018
Fund Project:
This work was supported by the National Natural Science Foundation of China (No.31601579), the Project of Key Research and Development of Hainan Province (No.ZDYF2016093) and the Research Foundation of Hainan Higher Education Institutions (No.Hnky2016ZD-1).

Abstract: The fatty acid contents in soybean oil, corn oil, coconut oil, sunflower seed oil and fish oil were detected by gas chromatography. According to the characteristics of fatty acids of raw oil and the relevant regulations of GB 10765-2010, the formula of 6 kinds of human milk lipid substitutes was designed by Matlab software and Excel linear programming. At the same time, the Schaal oven accelerated oxidation method was used to study the oxidation process of 6 formulations using synchronous fluorescence technique combined with traditional chemical reagents (oxidation index:peroxide value, value of fennel and total oxidation value). By statistical analysis of data, the statistical relationship between fluorescence intensity and oxidation index was explored. The results showed that the main fatty acids of coconut oil were lauric acid and myristic acid, and its saturated fatty acid content was the highest 93.75%±0.06%; while all the other oils were mainly composed of palmitic acid, linoleic acid and other components, wherein the fish oil had the highest content of unsaturated fatty acid. With the increase of oxidation time, the fluorescence intensity decreased gradually and the oxidation index value increased continuously. The correlation analysis and regression analysis results showed that the change of oxidation index and the fluorescence intensity of 6 samples had negative correlation, and the fluorescence intensity and the peroxide value and total oxidation value was quadratic linear correlation, and anisidine value as a linear correlation had strong correlation.

Key words:

Human milk lipid substitute
/ Schaal oven accelerated oxidation process
/ Oxidation index
/ Synchronous fluorescence

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同步荧光法监控人乳脂质替代品氧化过程

English

Monitoring of Oxidation Process of Human Milk Lipid Substitutes by Molecular Fluorescence Spectroscopy

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中国化学会秘书处

同步荧光法监控人乳脂质替代品氧化过程

English

Monitoring of Oxidation Process of Human Milk Lipid Substitutes by Molecular Fluorescence Spectroscopy

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

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CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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