高容量阴离子交换磁性微球的制备及其对蛋白质的吸附性能

引用本文: 贺茂芳, 张博, 唐一梅, 韩禄. 高容量阴离子交换磁性微球的制备及其对蛋白质的吸附性能[J]. 分析化学, 2018, 46(9): 1486-1492. doi: 10.11895/j.issn.0253-3820.181212 shu

Citation: HE Mao-Fang, ZHANG Bo, TANG Yi-Mei, HAN Lu. Preparation of High Capacity Anion Exchange Magnetic Microspheres and Its Adsorption of Protein[J]. Chinese Journal of Analytical Chemistry, 2018, 46(9): 1486-1492. doi: 10.11895/j.issn.0253-3820.181212 shu

高容量阴离子交换磁性微球的制备及其对蛋白质的吸附性能

西安医学院药学院, 西安医学院药物研究所, 西安 710021
基金项目:
本文系西安医学院博士科研启动基金（No.2016D0C06）和国家自然科学基金孵育项目（No.2017 GJFU08）资助

摘要: 为了获得高容量的阴离子交换吸附剂，本研究以Fe₃O₄磁性纳米粒子为基质，在其表面包覆聚多巴胺，然后与树枝状大分子聚乙烯亚胺反应，制得多氨基化磁性微球。此微球的离子交换容量为9.1 mmol/g。采用透射电镜、红外光谱和热重分析对材料进行了表征。以β-酪蛋白和牛血清蛋白（BSA）为模型蛋白，采用静态吸附法研究了此微球对蛋白质的吸附性能，在最佳条件下测定了微球对蛋白质的静态吸附等温线。结果表明，此微球对蛋白质的最佳吸附时间为2 h，缓冲溶液的最佳pH值为7.0。在此条件下，对β-酪蛋白和BSA的最大吸附容量分别为237.5和204.5 μg/mg，而对溶菌酶和核糖核酸酶A几乎不产生吸附。实验结果表明，此微球对酸性蛋白具有选择性，在蛋白质分离纯化方面具有良好的应用前景。

关键词:

English

Preparation of High Capacity Anion Exchange Magnetic Microspheres and Its Adsorption of Protein

College of Pharmacy, Institute of Drug Research, Xi'an Medical University, Xi'an 710021, China
Received Date: 31 March 2018
Revised Date: 19 July 2018
Fund Project:
This work was supported by the Doctor Research Supporting Foundation (No. 2016D0C06) and the National Natural Science Foundation Supporting Program of Xi'an Medical University (No.2017GJFU08).

Abstract: A high capacity anion exchange magnetic absorbent was prepared by immobilization of hyperbranched polyethyleneimine (PEI) on polydopamine-coated Fe₃O₄ microspheres (Fe₃O₄@pDA) and the ion exchange capacity was determined as 9.1 mmol/g. The absorbent was characterized by transmission electron microscope (TEM), infrared spectroscopy (IR) and thermogravimetric analysis (TGA). Moreover, the adsorption behavior of the absorbent for protein was carefully investigated by static adsorption using bovine serum albumin (BSA) and β-casein as models and then the static isotherms were determined under the optimum conditions. It was shown that the highest adsorption amount for protein was obtained when the adsorption time was 2 h and the pH was 7.0. Under the optimum conditions, the saturated adsorption capacity towards β-casein and BSA was determined as high as 237.5 and 204.5 μg/mg, respectively. Comparatively, the adsorbent exhibited little adsorption towards lysozyme and Rnase A, showing highly selectivity towards acidic protein. Therefore, the adsorbent exhibited potential in the separation and purification of protein from real samples.

Key words:

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高容量阴离子交换磁性微球的制备及其对蛋白质的吸附性能

English

Preparation of High Capacity Anion Exchange Magnetic Microspheres and Its Adsorption of Protein

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中国化学会秘书处

高容量阴离子交换磁性微球的制备及其对蛋白质的吸附性能

English

Preparation of High Capacity Anion Exchange Magnetic Microspheres and Its Adsorption of Protein

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

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CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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