猪粪厌氧发酵的有机酸代谢与微生物群落相关性分析

引用本文: 张丹志, 尹强, 徐文静, 方勇, 焦郑同, 马诗淳, 刘祚军, 章力干. 猪粪厌氧发酵的有机酸代谢与微生物群落相关性分析[J]. 分析化学, 2022, 50(8): 1260-1268. doi: 10.19756/j.issn.0253-3820.221225 shu

Citation: ZHANG Dan-Zhi, YIN Qiang, XU Wen-Jing, FANG Yong, JIAO Zheng-Tong, MA Shi-Chun, LIU Zuo-Jun, ZHANG Li-Gan. Relationship Between Organic Acid Metabolites and Microbial Communities During Anaerobic Digestion of Pig Manure[J]. Chinese Journal of Analytical Chemistry, 2022, 50(8): 1260-1268. doi: 10.19756/j.issn.0253-3820.221225 shu

猪粪厌氧发酵的有机酸代谢与微生物群落相关性分析

张丹志 ^1,2, ,
尹强 ^2, ,
徐文静 ^2, ,
方勇 ^2, ,
焦郑同 ^2, ,
马诗淳 ^3, ,
刘祚军 ^{2,
,} ,
章力干 ^{1,
,}

1.
安徽农业大学资源与环境学院, 合肥 230036;
2.
安徽省农业科学院农业工程研究所, 合肥 230001;
3.
农业农村部沼气科学研究所, 成都 610041

通讯作者: 刘祚军,E-mail:zhangligan1965@163.com; 章力干,E-mail:liuzj1029@163.com

基金项目:
安徽省农业科学院创新团队项目(No.2022YL036)和农业农村部农村可再生能源开发利用重点实验室开放课题项目(No.2019011)资助。 # 共同第一作者

摘要: 有机酸代谢和微生物菌群结构稳定性是维持厌氧消化系统高效运行的关键。为研究该理化物质代谢与微生物群落变化之间的关系,本研究以猪粪发酵体系为例,设置3个搅拌梯度(0、80和200 r/min),分别代表无搅拌、适度搅拌和过度搅拌条件,监测猪粪厌氧发酵过程中挥发性脂肪酸等理化性质的变化,再结合高通量测序技术,进一步分析微生物种群多样性变化。结果表明,发酵体系以厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidetes)、变形菌门(Proteobacteria)和广古菌门(Euryarchaeota)为优势菌。适度搅拌可在一定程度上促进微生物分解有机质的效率,形成稳定高效的微生物群落,其中,总固体、挥发性固体和化学需氧量去除率分别达到79.15%、63.63%和87.89%。有机质分解代谢产物以乙酸和丙酸为主,其浓度与拟杆菌门(Bacteroidetes)丰度呈正相关性,与变形菌门(Proteobacteria)呈负相关性。过度搅拌使产氢产乙酸菌(如互养菌门(Synergistetes))丰度增加,引起耗氢产乙酸菌酸杆菌门(Acidobacteria)丰度随之增加,导致乙酸和丙酸浓度分别提高21.09%和6.44%,氢营养型产甲烷菌甲烷粒菌属(Methanocorpusculum)丰度随之增加31.95%,但甲烷产量反而下降,说明过度搅拌显著提高了乙酸和丙酸浓度,促进产甲烷途径由乙酸裂解型向低效的乙酸氧化产甲烷转化。本研究结果对于理解猪粪厌氧消化过程的有机酸代谢与产甲烷途径的调控具有重要的参考意义。

关键词:

English

Relationship Between Organic Acid Metabolites and Microbial Communities During Anaerobic Digestion of Pig Manure

1.
College of Resources and Environment, Anhui Agricultural University, Hefei 230036, China;
2.
Agricultural Engineering Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230001, China;
3.
Biogas Institute of Ministry of Agriculture and Rural Affairs, Chengdu 610041, China

Corresponding author: LIU Zuo-Jun, ; ZHANG Li-Gan,

Received Date: 09 May 2022
Revised Date: 29 May 2022
Fund Project:
Supported by the Project of Science and Technology Innovation Team in Anhui Academy of Agricultural Sciences (No.2022YL036) and the Key Laboratory of Development and Application of Rural Renewable Energy, Ministry of Agriculture and Rural Affairs of the People's Republic of China (No.2019011).

Abstract: The stability of organic acid metabolites and microbial communities structure contributed to improve the efficiency of anaerobic digestion in the large-scale biogas project. To study the relationship between the organic acid metabolites and microbial communities, three stirring speeds including 0, 80 and 200 r/min in a 4-L laboratory fermenter were set to monitor the physical and chemical qualitative changes such as volatile fatty acids during pig manure digestion. Combined with high-throughput sequencing technology, the evolution of microbial diversity and community structure in anaerobic digestion system was organically related to the metabolism of organic acids. The results indicated that, Firmicutes, Bacteroides, Proteobacteria and Euryarchaeota were the main dominant bacterial taxa in the microbial community structure. Firstly, moderate strring could improve the efficiency of anaerobic digestion and the stability of microbial community in the fermentation system in which the removal rate of total solids, volatile solids and chemical oxygen demand reached 79.15%, 63.63% and 87.89%, respectively. Moreover, as the main organic acid metabolites, the concentration of the acetic acid and propionic acid were positively correlated with Bacteroidetes and negatively correlated with Proteobacteria. Secondly, with high-speed stirring measure, the increased abundance of hydrogen-producing acetogens Synergistetes led to an increase in the abundance of Acidobacteria. Meanwhile, the concentration of acetic acid and propionic acid was increased by 21.09% and 6.44% respectively, and the abundance of hydrogenotrophic methanogens Methanocorpusculum was increased by 31.95%. Interestingly, the yield of methane had actually fallen. These data showed that excessive stirring during anaerobic digestion increased the concentrations of acetic acid and propionic acid significantly, and promoted the switchcover of methanogenic pathway from acetic acid pyrolysis pathway to less efficient acetic acid oxidation pathway. The results provided a new perspective for understanding the regulation between organic acid metabolism and methanogenic pathway in anaerobic digestion of pig manure.

Key words:

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

猪粪厌氧发酵的有机酸代谢与微生物群落相关性分析

通讯作者: 刘祚军,E-mail:zhangligan1965@163.com; 章力干,E-mail:liuzj1029@163.com

English

Relationship Between Organic Acid Metabolites and Microbial Communities During Anaerobic Digestion of Pig Manure

Corresponding author: LIU Zuo-Jun, ; ZHANG Li-Gan,

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

猪粪厌氧发酵的有机酸代谢与微生物群落相关性分析

通讯作者: 刘祚军,E-mail:zhangligan1965@163.com; 章力干,E-mail:liuzj1029@163.com

English

Relationship Between Organic Acid Metabolites and Microbial Communities During Anaerobic Digestion of Pig Manure

Corresponding author: LIU Zuo-Jun, ; ZHANG Li-Gan,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs