引用本文:
Hai Liang CUI, Yong YU, Wan Chun CHEN, Qi KANG. Study of Growth Mechanism of Lysozyme Crystal by Batch Crystallization Method[J]. Chinese Chemical Letters,
2006, 17(1): 101-104.
Citation: Hai Liang CUI, Yong YU, Wan Chun CHEN, Qi KANG. Study of Growth Mechanism of Lysozyme Crystal by Batch Crystallization Method[J]. Chinese Chemical Letters, 2006, 17(1): 101-104.
Citation: Hai Liang CUI, Yong YU, Wan Chun CHEN, Qi KANG. Study of Growth Mechanism of Lysozyme Crystal by Batch Crystallization Method[J]. Chinese Chemical Letters, 2006, 17(1): 101-104.
Study of Growth Mechanism of Lysozyme Crystal by Batch Crystallization Method
摘要:
The lysozyme crystals were made by batch crystallization method and the distribution of aggregate in solution were measured by dynamic light scattering. The results showed that the dimension of aggregate increased with the increase of the concentration of lysozyme and NaC1, lysozyme molecules aggregated gradually in solution and finally arrived at balance each other.The higher the concentrations of lysozyme and NaC1 were, the faster the growth rate of (110) face was. The growth rates of lysozyme crystal were obtained by a Zeiss microscope, and the effective surface energy (α) of growing steps were calculated about 4.01×l0-8 J·cm-2 according to the model of multiple two-dimensional nucleation mechanism.
English
Study of Growth Mechanism of Lysozyme Crystal by Batch Crystallization Method
Abstract:
The lysozyme crystals were made by batch crystallization method and the distribution of aggregate in solution were measured by dynamic light scattering. The results showed that the dimension of aggregate increased with the increase of the concentration of lysozyme and NaC1, lysozyme molecules aggregated gradually in solution and finally arrived at balance each other.The higher the concentrations of lysozyme and NaC1 were, the faster the growth rate of (110) face was. The growth rates of lysozyme crystal were obtained by a Zeiss microscope, and the effective surface energy (α) of growing steps were calculated about 4.01×l0-8 J·cm-2 according to the model of multiple two-dimensional nucleation mechanism.
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Key words:
- Protein crystal
- / growth rate
- / dynamic light scattering
- / lysozyme
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