液相色谱-质谱联用技术检测大鼠血浆、肾上腺及下丘脑中神经递质含量

引用本文: 李慧, 鲁文慧, 焦丽丽, 吴巍, 宋凤瑞, 刘淑莹. 液相色谱-质谱联用技术检测大鼠血浆、肾上腺及下丘脑中神经递质含量[J]. 分析化学, 2018, 46(4): 586-593. doi: 10.11895/j.issn.0253-3820.171472 shu

Citation: LI Hui, LU Wen-Hui, JIAO Li-Li, WU Wei, SONG Feng-Rui, LIU Shu-Ying. Simultaneous Determination of Six Neurotransmitters in Rat Plasma, Adrenal Gland and Hypothalamus with Liquid Chromatography-Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, 2018, 46(4): 586-593. doi: 10.11895/j.issn.0253-3820.171472 shu

液相色谱-质谱联用技术检测大鼠血浆、肾上腺及下丘脑中神经递质含量

1.
中国科学院长春应用化学研究所, 长春 130022;
2.
长春中医药大学吉林省人参科学研究院, 长春 130117
基金项目:
本文系吉林省科技发展计划基金项目（Nos.20160308YY，20160101334JC）和吉林省自然科学基金项目（No.20180520062JH）资助

摘要: 建立了一种液相色谱-质谱联用技术同时检测γ-氨基丁酸（GABA）、5-羟色胺（5-HT）、酪氨酸（L-tyr）、多巴胺（DA）、去甲肾上腺素（NE）和肾上腺素（E）6种神经递质的方法，并将本方法用于大鼠肾上腺、下丘脑及血浆中神经递质的检测。以丹磺酰氯为衍生化试剂，以5-羟基色氨酸和咖啡酸为内标，采用UltiMate 3000 RSLC色谱系统、Thermo C₁₈色谱柱（50 mm×3 mm，2.7 μm），流动相为0.1%甲酸和甲醇溶液梯度洗脱，流速0.2 mL/min，进样量2 μL，多反应检测（MRM），正离子模式。GABA、5-HT、L-Tyr、DA、NE、E线性检测范围分别为0.26~620.80 μmol/L、0.034~11.20 μmol/L、1.20~88.00 μmol/L、0.03~41.02 μmol/L、0.01~47.20 μmol/L、0.01~90.24 μmol/L，加标回收率为91.16%~116.20%。本方法可快速准确检测大鼠肾上腺、血浆及下丘脑中神经递质含量，为药理实验的中的指标分析提供了检测技术支持。

关键词:

English

Simultaneous Determination of Six Neurotransmitters in Rat Plasma, Adrenal Gland and Hypothalamus with Liquid Chromatography-Tandem Mass Spectrometry

1.
Changchun Institute of Applied Chemistry Chinese Academy of Sciences, Changchun 130022, China;
2.
Changchun University of Chinese Medicine, Jilin Ginseng Academy, Changchun 130117, China
Received Date: 02 December 2017
Revised Date: 05 January 2018

Abstract: A rapid Liquid chromatography-tandem mass spectrometry method was developed for the simultaneous determination of six neurotransmitters in rat plasma, adrenal gland and hypothalamus, including γ-aminobutyric acid (GABA), serotonin(5-HT), tyrosine(L-Tyr), dopamine(DA), norepinephrine(NE) and epinephrine(E). This method was used for the neurotransmitters detection in rat adrenal, blood and hypothalamus. The samples were pre-column derivatized with dansyl chloride, and 5-HTP and CA were used as internal standards. A Thermo C₁₈ column (50 mm×3 mm, 2.7 μm) was used for sample separation with 0.1% aqueous formic acid solution as phase A and methanol as phase B at a flow rate of 0.2 mL/min, and the injection volume was set at 2 μL. The detection was carried out with multi-reaction monitoring (MRM) in electron spray ionization (ESI) positive mode. The linear ranges for detection of GABA, 5-HT, L-Tyr, DA, NE and E were 0.26-620.80 μmol/L, 0.03-11.20 μmol/L, 1.20-88.00 μmol/L, 0.03-41.02 μmol/L, 0.01-47.20 μmol/L, 0.01-90.24 μmol/L, respectively, with recoveries varying from 91.16% to 116.20%. This method can detect the neurotransmitters rapidly and accurately, providing a platform for the determination of neurotransmitters in rat plasma, adrenal gland and hypothalamus in pharmacological experiments.

Key words:

Liquid chromatography-tandem mass spectrometry
/ Adrenal
/ Blood
/ Hypothalamus
/ Neurotransmitters

1. [1]
  Brichta L, Greengard P, Flajolet M. Trends Neurosci.,2013,36(9):543-554
2. [2]
  Muramatsu I, Uwada J, Masuoka T, Yoshiki H, Sada K, Lee K S, Nishio M, Ishibashi T, Taniguchi T. J. Neurochem.,2017,143(1):76-86
3. [3]
  Hammer H, Bader B M, Ehnert C, Bundgaard C, Bunch L, Hoestgaard J K, Schroeder O H, Bastlund J F, Gramowski V A, Jensen A A. Mol. Pharmacol.,2015,88(2):401-420
4. [4]
  Buonomo F C, Zimmermann N G, Lauterio T J, Scanes C G. Gen. Comp. Endocrinol.,1984,54(3):360-371
5. [5]
  HE Yu-Chen, ZHANG Bo, ZHAI Wei, NING Jie, QUAN Hong-Yu, XIA Yu, YAO Yuan, HAN Mei. J. Third. Mil. Med. Univ.,2014,36(8):806-810 和昱辰, 张波, 瞿玮, 宁洁, 全弘宇, 夏宇, 姚远, 韩梅.第三军医大学学报,2014,36(8):806-810
6. [6]
  WEI Li-Li, CHEN Hong, ZHONG Ming, DU Juan, LIU Fei, LIU Chun-Yang. Chin. J. Pharm. Anal.,2011,31(3):467-470 魏丽丽, 陈虹, 钟明, 杜娟, 刘斐, 刘春阳.药物分析杂志,2011,31(3):467-470
7. [7]
  WANG Meng-Meng, DU Wang-Chun, SHEN Jie, DONG Yi, WEI Weng-Shi, SONG Zhong-Juan. J. Chin. Pharm. Sci.,2013,22(3):239-243 王萌萌, 杜望春, 沈杰, 董毅, 魏文石, 宋钟娟.中国药学(英文版),2013,22(3):239-243
8. [8]
  CHEN Shu-Jiao, LI Can-Dong, LIAO Ling-Hong, DING Shan-Shan, YU Jie, KANG Jie, SHEN Jian-Ying, ZHANG Ling-Yuan. Chin. J. Tradit. Chin. Med. Pharm.,2013,28(5):1232-1236 陈淑娇, 李灿东, 廖凌虹, 丁姗姗, 俞洁, 康洁, 沈建英, 张凌媛.中华中医药杂志,2013,28(5):1232-1236
9. [9]
  WANG Qian-Qian, ZHANG Yan, MENG Lu-Lu, PI Zi-Fng, LIU Shu, Song Feng-Rui, LIU Zhi-Qiang. Chinese J. Anal.Chem.,2015,43(11):1761-1765 王倩倩, 张艳, 孟璐璐, 皮子凤, 刘舒, 宋凤瑞, 刘志强.分析化学,2015,43(11):1761-1765
10. [10]
  Cai H L, Zhu R H, Li H D.Anal. Biochem.,2010,396(1):103-111
11. [11]
  HUANG Xin, LI Shuai-Ping, ZHANG Yong, LIU Shu-Ying. Chinese J. Anal. Chem.,2016,44(11):1652-1658 黄鑫, 李帅坪, 张勇, 刘淑莹.分析化学,2016,44(11):1652-1658
12. [12]
  Yang Z L, Li H, Wang B, Liu S Y. J. Chromatogr. B,2016,(1012-1013):79-88
13. [13]
  WANG Qian-Qian, ZHANG Yan, MENG Lu-Lu, PI Zi-Feng, LIU Shu, SONG Feng Rui, LIU Zhi-Qiang.Chinese J. Anal. Chem., 2015,43(11):1754-1760 王倩倩, 张艳, 孟璐璐, 皮子凤, 刘舒, 宋凤瑞, 刘志强.分析化学,2015,43(11):1754-1760
14. [14]
  YANG Shuai, PAN Ye, SONG Yan Qi, GAO Shan, CAI Xue-Meng, GAO Shu-Ming, YU Chun-Quan. Chin. Tradit. Herbal. Drugs,2016,47(23):4218-4223 杨帅, 潘晔, 宋彦奇, 高杉, 蔡雪朦, 高树明, 于春泉.中草药,2016,47(23):4218-4223
15. [15]
  LIU Sheng-Jin, WU Lu-Ting, WU De-Kang, WANG Rui, YU Bin, WANG Yu-Hua, LIU Pei, YANG Wen-Guo. J. Chin. Mass Spectr. Soc.,2016,37(6):533-541 刘圣金, 吴露婷, 吴德康, 王瑞, 喻斌, 王宇华, 刘培, 杨文国. 质谱学报, 2016,37(6):533-541
16. [16]
  Hua L C, Rong H Z, Huan D L, Zhang J, Li L F. J. Chromatogr. B,2011, 879(21):1993-1999
17. [17]
  Wei B, Li Q. Fan R, Su D, Chen X, Jia Y, Bi K. J. Pharm. Biomed. Anal.,2014,88(25):416-422
18. [18]
  Zhai X J, Chen F, Zhu C R, Lu Y N. Biomed. Chromatogr.,2015,29(11):1737-1743
19. [19]
  LI Hui, LIU Shu-Ying, WANG Bing.Acta Pharm. Sin., 2014,49(5):569-575 李慧, 刘淑莹, 王冰.药学学报,2014,49(5):569-575
20. [20]
  Gonzalez R R, Fernandez R F, Vidal J L, Frenich A G, Perez M L. J. Neurosci. Methods,2011,198 (2):187-194
21. [21]
  Zheng X, Kang A, Dai C, Liang Y, Xie T, Xie L, Peng Y, Wang G, Hao H. Anal. Chem.,2012,84(22):10044-10051
22. [22]
  Tareke E, Bowyer J F, Doerge D R. Rapid Commun. Mass Spectrom.,2007,21(23):3898-3904

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液相色谱-质谱联用技术检测大鼠血浆、肾上腺及下丘脑中神经递质含量

English

Simultaneous Determination of Six Neurotransmitters in Rat Plasma, Adrenal Gland and Hypothalamus with Liquid Chromatography-Tandem Mass Spectrometry

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

液相色谱-质谱联用技术检测大鼠血浆、肾上腺及下丘脑中神经递质含量

English

Simultaneous Determination of Six Neurotransmitters in Rat Plasma, Adrenal Gland and Hypothalamus with Liquid Chromatography-Tandem Mass Spectrometry

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

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