离子色谱-高分辨质谱法快速筛查乳制品中的酸度调节剂

引用本文: 云环, 刘鑫, 崔杰, 杨婧, 刘影. 离子色谱-高分辨质谱法快速筛查乳制品中的酸度调节剂[J]. 色谱, 2017, 35(8): 886-890. doi: 10.3724/SP.J.1123.2017.04012 shu

Citation: YUN Huan, LIU Xin, CUI Jie, YANG Jing, LIU Ying. Rapid screening of acidity regulators in dairy by ion chromatography-high resolution mass spectrometry[J]. Chinese Journal of Chromatography, 2017, 35(8): 886-890. doi: 10.3724/SP.J.1123.2017.04012 shu

离子色谱-高分辨质谱法快速筛查乳制品中的酸度调节剂

云环 ^1, ,
刘鑫 ^1, ,
崔杰 ^2, ,
杨婧 ^1, ,
刘影 ^2,

1.
北京出入境检验检疫局, 北京 100026;
2.
北京工业技师学院, 北京 100023
基金项目:
国家质检总局科研项目（2015IK013）.

摘要: 建立了离子色谱-高分辨质谱（IC-HRMS）串联筛查乳制品中多种酸度调节剂的方法。乳制品样品用KOH溶液（pH调至7~8）提取，经Oasis MAX SPE柱净化，用Dionex IonPac AS11-HC柱（250 mm×4 mm）分离，Orbitrap检测器全扫描模式检测。以6种有机酸为例进行验证，在0.01~5.00 mg/L范围内线性相关系数均大于0.99。实际样品的加标回收率为74.3%~115.5%，相对标准偏差（RSD）为0.64%~4.81%（n=9）。将该法用于市售乳制品中酸度调节剂的筛查，在样品中可以筛查到苹果酸、柠檬酸、丁二酸、乳酸和己二酸。该方法快速、简便，可用于乳制品中多种酸度调节剂的定性筛查。

关键词:

English

Rapid screening of acidity regulators in dairy by ion chromatography-high resolution mass spectrometry

YUN Huan ^1, ,
LIU Xin ^1, ,
CUI Jie ^2, ,
YANG Jing ^1, ,
LIU Ying ^2,

1.
Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing 100026, China;
2.
Beijing Industrial Technician College, Beijing 100023, China
Received Date: 11 April 2017
Fund Project:
Science and Technology Planning Preject of General Administration of Quality Supervision, Inspection and Quarantine (AQSIQ) (No. 2015IK013).

Abstract: A method for screening of acidity regulators in dairy based on ion chromatography-high resolution mass spectrometry technology (IC-HRMS) was set up. The dairy samples were extracted by KOH (pH 7-8) and Oasis MAX SPE column, and separated by a Dionex IonPac AS11-HC column (250 mm×4 mm). All the acidity regulators were detected by Orbitrap full scan mode. Taking six organic acids as an example, the calibration curves showed good linearities in the range of 0.05-5.00 mg/L, and the correlation coefficients (r) were higher than 0.99. By detecting the spiked samples, the recoveries were in the range of 74.3%-115.5% with the relative standard deviations (RSDs) between 0.64% and 4.81%. Malic acid, citric acid, lactic acid, succinic acid and adipic acid could be detected by IC-HRMS in the commercial dairy samples. The results indicate that the method is simple, rapid and suitable for the qualitative screening of acidity regulators in dairy products.

Key words:

ion chromatography-high resolution mass spectrometry (IC-HRMS)
/ rapid screening
/ acidity regulators
/ dairy

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沈阳化工大学材料科学与工程学院沈阳 110142

离子色谱-高分辨质谱法快速筛查乳制品中的酸度调节剂

English

Rapid screening of acidity regulators in dairy by ion chromatography-high resolution mass spectrometry

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离子色谱-高分辨质谱法快速筛查乳制品中的酸度调节剂

English

Rapid screening of acidity regulators in dairy by ion chromatography-high resolution mass spectrometry

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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