引用本文:
Fei WANG, Xiong CHEN, Qian CHEN, Xin Chen QIN, Zhi Xiao LI. HPLC Determination of Neurotoxin β-N-oxalyl-L-α,β-diaminopropionic acid and Its α-Isomer in Lathyrus sativus by Precolumn Derivatization with 1-Fluoro-2,4-dinitrobenzene[J]. Chinese Chemical Letters,
2000, 11(5): 435-438.
Citation: Fei WANG, Xiong CHEN, Qian CHEN, Xin Chen QIN, Zhi Xiao LI. HPLC Determination of Neurotoxin β-N-oxalyl-L-α,β-diaminopropionic acid and Its α-Isomer in Lathyrus sativus by Precolumn Derivatization with 1-Fluoro-2,4-dinitrobenzene[J]. Chinese Chemical Letters, 2000, 11(5): 435-438.
Citation: Fei WANG, Xiong CHEN, Qian CHEN, Xin Chen QIN, Zhi Xiao LI. HPLC Determination of Neurotoxin β-N-oxalyl-L-α,β-diaminopropionic acid and Its α-Isomer in Lathyrus sativus by Precolumn Derivatization with 1-Fluoro-2,4-dinitrobenzene[J]. Chinese Chemical Letters, 2000, 11(5): 435-438.
HPLC Determination of Neurotoxin β-N-oxalyl-L-α,β-diaminopropionic acid and Its α-Isomer in Lathyrus sativus by Precolumn Derivatization with 1-Fluoro-2,4-dinitrobenzene
摘要:
A rapid and simple method is presented for determining β-N-oxalyl-α,β-diaminopropionic acid (β-ODAP) and its much less toxic α-isomer (α-ODAP) in Lathyrus sativus. Seed and foliage extracts of Lathyrus sativus were treated with 1-fluoro-2,4-dinitrobenzene (FDNB) and a reversed-phase high-performance liquid chromatographic method for the separation of the derivatives in the pmol range is reported.
English
HPLC Determination of Neurotoxin β-N-oxalyl-L-α,β-diaminopropionic acid and Its α-Isomer in Lathyrus sativus by Precolumn Derivatization with 1-Fluoro-2,4-dinitrobenzene
Abstract:
A rapid and simple method is presented for determining β-N-oxalyl-α,β-diaminopropionic acid (β-ODAP) and its much less toxic α-isomer (α-ODAP) in Lathyrus sativus. Seed and foliage extracts of Lathyrus sativus were treated with 1-fluoro-2,4-dinitrobenzene (FDNB) and a reversed-phase high-performance liquid chromatographic method for the separation of the derivatives in the pmol range is reported.
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