无线磁弹性适配体传感器用于检测癌胚抗原

引用本文: 桑胜波, 郭星, 张益霞, 郭锦玉, 李红梅, 李艳萍, 张强, 王涛. 无线磁弹性适配体传感器用于检测癌胚抗原[J]. 分析化学, 2021, 49(5): 718-725. doi: 10.19756/j.issn.0253-3820.191695 shu

Citation: SANG Sheng-Bo, GUO Xing, ZHANG Yi-Xia, GUO Jin-Yu, LI Hong-Mei, LI Yan-Ping, ZHANG Qiang, WANG Tao. A Wireless Magnetoelastic Aptasensor for Detection of Carcinoembryonic Antigen[J]. Chinese Journal of Analytical Chemistry, 2021, 49(5): 718-725. doi: 10.19756/j.issn.0253-3820.191695 shu

无线磁弹性适配体传感器用于检测癌胚抗原

桑胜波 ^1, ,
郭星 ^{1,
,} ,
张益霞 ^1,2, ,
郭锦玉 ^1, ,
李红梅 ^1, ,
李艳萍 ^1, ,
张强 ^1, ,
王涛 ^3,

1.
太原理工大学信息与计算机学院, 微纳系统研究中心, 传感器和智能控制系统教育部重点实验室, 晋中 030600;
2.
太原理工大学力学学院, 晋中 030600;
3.
山西白求恩医院, 太原 030032

通讯作者: 郭星,E-mail:guo_xing163@163.com

基金项目:
国家自然科学基金项目（Nos.51705354，61703298，81602506，51975400）和山西省高等学校科技创新项目（No.2020L0076）资助。

摘要: 在磁弹性芯片表面修饰癌胚抗原适配体（CEA-apt），构建了新型磁弹性适配体传感器，用于癌胚抗原（CEA）的高灵敏无线检测。基于磁致伸缩效应，信号的激发与传输可以通过电磁场进行，可实现无线检测，并用于活体分析。扫描电子显微镜（SEM）、原子力显微镜（AFM）、能谱（EDS）表征结果表明，磁弹性适配体传感器可以特异性结合CEA。在CEA-apt的最佳修饰浓度下，磁弹性适配体传感器的共振频率偏移量与CEA浓度在1~100 ng/mL范围内呈线性关系，检出限为0.09 ng/mL。通过与临床采用的化学发光免疫测定法对比，验证了磁弹性适配体传感器的可行性。磁弹性适配体传感器表现出良好的特异性、稳定性和可重复性，为癌症的临床诊断提供了技术支持。

关键词:

English

A Wireless Magnetoelastic Aptasensor for Detection of Carcinoembryonic Antigen

1.
MicroNano System Research Center, Key Lab of Advanced Transducers and Intelligent Control System of the Ministry of Education, College of Information and Computer, Taiyuan University of Technology, Jinzhong 030600, China;
2.
Institute of Applied Mechanics & National Demonstration Center for Experimental Mechanics Education, College of Mechanical and Vehicle Engineering, Taiyuan University of Technology, Jinzhong 030600, China;
3.
Shanxi Academy of Medical Sciences & Shanxi Bethune Hospital, Taiyuan 030032, China

Corresponding author: GUO Xing, guo_xing163@163.com

Received Date: 25 November 2019
Revised Date: 03 September 2020
Fund Project:
Supported by the National Natural Science Foundation of China (Nos.51705354, 61703298, 81602506, 51975400) and the Science and Technology Innovation Project of Colleges and Universities in Shanxi Province (No.2020L0076).

Abstract: A novel magnetoelastic (ME) aptasensor containing aptamer functionalized gold-coated ME chip was developed for carcinoembryonic antigen (CEA) detection. Due to its magnetostrictive properties, the signals could be wirelessly detected through magnetic field, which made it potentially suitable for in vivo analysis. The conjugation of aptamers and CEA was successfully confirmed by scanning electron microscopy, atomic force microscopy and energy dispersive spectroscopy. With the optimum modification concentration of CEA-Apt, the ME aptasensor demonstrated a linear range from 1 ng/mL to 100 ng/mL towards CEA concentration with a detection limit (LOD) of 0.09 ng/mL. Clinical chemiluminescence assay (CA method) was carried out to certify the feasibility of the ME aptasensor. The ME aptasensor exhibited excellent selectivity, stability and repeatability towards CEA, which provided a promising method for clinical diagnosis of cancer.

Key words:

1. [1]
  CHEN W, ZHENG R, BAADE P D, ZHANG S, ZENG H. CA-Cancer J. Clin., 2016, 66(2):115-132.
2. [2]
  XIAO K, WANG K, QIN W, HOU Y LU W, XU H, CUI D. Talanta, 2017, 164:463-469.
3. [3]
  AGGARWAL C, MEROPOL N J, PUNT C J, IANNOTTI N, SAID MAN B H, SABBATH K D, GAD RAIL N Y, PICUS J, MORSE M A, MITCHELL E. Ann. Oncol., 2013, 24(2):420-428.
4. [4]
  GUO J, YU J, SONG X, MI H. Open Med., 2017, 12:131-137.
5. [5]
  YANG A P, LIU J, LEI H Y, ZHANG Q W, ZHAO L, YANG G H. Clin. Chim. Acta, 2014, 437:183-186.
6. [6]
  JÄRVISALO J, HAKAMA M, KNEKT P, STENMAN U H, LEINO A, TEPPO L, MAATELA J, AROMAA A. Cancer, 1993, 71:1982-1988.
7. [7]
  NARAYAN T, KUMAR S, KUMAR S, AUGUSTINE S, YADAV B K, MALHOTRA B D. Talanta, 2019, 201:465-473.
8. [8]
  FERRARI M. Nat. Rev. Cancer, 2005, 5:161-171.
9. [9]
  URVA S R, YANG V C, BALTHASAR J P.J. Immunoassay Immunochem., 2009, 30:418-427.
10. [10]
  LI M, CUSHING S K, ZHANG J, SURI S, EVANS R, PETROS W P, GIBSON L F, MA D, LIU Y, WU N. ACS Nano, 2013, 7:4967-4976.
11. [11]
  GU X, SHE Z, MA T, TIAN S, KRAATZ H B. Biosens. Bioelectron., 2018, 102:610-616.
12. [12]
  WANG Y, DAN Y, ZHANG H, FAN Y, PANG D. Sens. Actuators, B, 2018, 255:125-132.
13. [13]
  GAO X, ZHANG Y, WU Q, CHEN H, CHEN Z, LIN X. Talanta, 2011, 85:1980-1985.
14. [14]
  ZHANG X, DING S N. Sens. Actuators, B, 2017, 240:1123-1133.
15. [15]
  LI H, SHI L, SUN D E, LI P, LIU Z. Biosens. Bioelectron., 2016, 86:791-798.
16. [16]
  WU K, CHU C, MA C, YANG H, SONG X R, YU J H. Sens. Actuators, B, 2015, 206:43-49.
17. [17]
  QIU Z, SHU J, TANG D. Chem. Commun., 2018, 54:7199-7202.
18. [18]
  QIU Z, SHU J, TANG D. Anal. Chem., 2017, 90:1021-1028.
19. [19]
  ZHOU Q, LIN Y, ZHANG K, LI M, TANG D. Biosens. Bioelectron., 2018, 101:146-152.
20. [20]
  GUO X, SANG S, GUO J, JIAN A, DUAN Q, JI J, ZHANG Q, ZHANG W. Sci. Rep., 2017, 7:15626.
21. [21]
  STOYANOV P G, GRIMES C A. Sens. Actuators, A, 2000, 80:8-14.
22. [22]
  SCHMIDT S, GRIMES C A. Sens. Actuators, A, 2001, 94:189-196.
23. [23]
  CHEN L, LI J, THANHTHUY T T, ZHOU L, HUANG C, YUAN L, CAI Q. Biosens. Bioelectron., 2014, 52:427-432.
24. [24]
  LIN H, CHEN Z, LU Q, CAI Q, GRIMES C A. Sens. Actuators, B, 2010, 146:154-159.
25. [25]
  PARK M K, PARK J W, WIKLE H C, CHIN B A. Sens. Actuators, B, 2013, 176:1134-1140.
26. [26]
  HIREMATH N, GUNTUPALLI R, VODYANOY V, CHIN B A, PARK M K. Sens. Actuators, B, 2015, 210:129-136.
27. [27]
  ZHANG Y, GUO X, FAN L, ZHANG Q, SANG S. Nanoscale Res. Lett., 2018, 13:258.
28. [28]
  YU X, CHEN F, WANG R, LI Y. J. Biotechnol., 2018, 266:39-49.
29. [29]
  HASHEMI TABAR G, SMITH C. World Appl. Sci. J., 2010, 8.
30. [30]
  JIANG X, WANG H, WANG H, ZHUO Y, YUAN R, CHAI Y. Anal.Chem., 2017, 89:4280-4286.
31. [31]
  SETTU K, LIU J T, CHEN C J, TSAI J Z. Anal. Biochem., 2017, 534:99-107.
32. [32]
  YANG X, ZHUO Y, ZHU S, LUO Y, FENG Y, XU Y. Biosens. Bioelectron., 2015, 64:345-351.
33. [33]
  SHAHBAZI N, HOSSEINKHANI S, RANJBAR B. Sens. Actuators, B, 2017, 253:794-803.
34. [34]
  MAHMOUDIAN M, ALIAS Y, BASIRUN W, WOI P M. Electrochim. Acta, 2015, 169:126-133.
35. [35]
  ZHENG D, XIONG J, GUO P, LI Y, WANG S, CU H. Mater. Res. Bull., 2014, 59:411-415.
36. [36]
  LI R, FENG F, CHEN Z, BAI Y, CUO F. Talanta, 2015, 140:143-149.
37. [37]
  SHAHBAZI N, HOSSEINKHANI S, RANJBAR B. Sens. Actuators, B, 2017, 253:794-803.
38. [38]
  WANG J, CAO F, HE S, XIA Y, LIU X Y, CHEN W W. Talanta, 2018, 176:444-449.

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

无线磁弹性适配体传感器用于检测癌胚抗原

通讯作者: 郭星,E-mail:guo_xing163@163.com

English

A Wireless Magnetoelastic Aptasensor for Detection of Carcinoembryonic Antigen

Corresponding author: GUO Xing, guo_xing163@163.com

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

无线磁弹性适配体传感器用于检测癌胚抗原

通讯作者: 郭星,E-mail:guo_xing163@163.com

English

A Wireless Magnetoelastic Aptasensor for Detection of Carcinoembryonic Antigen

Corresponding author: GUO Xing, guo_xing163@163.com

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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