QuEChERS-高效液相色谱-串联质谱法测定动物源性食品中3种镇静剂残留

引用本文: 邹游, 邵琳智, 陈思敏, 欧阳少伦, 林峰. QuEChERS-高效液相色谱-串联质谱法测定动物源性食品中3种镇静剂残留[J]. 色谱, 2017, 35(8): 801-807. doi: 10.3724/SP.J.1123.2017.03043 shu

Citation: ZOU You, SHAO Linzhi, CHEN Simin, OUYANG Shaolun, LIN Feng. Determination of three tranquillizer residues in animal foods by QuEChERS-high performance liquid chromatography-tandem mass spectrometry[J]. Chinese Journal of Chromatography, 2017, 35(8): 801-807. doi: 10.3724/SP.J.1123.2017.03043 shu

QuEChERS-高效液相色谱-串联质谱法测定动物源性食品中3种镇静剂残留

广东出入境检验检疫局检验检疫技术中心, 广东广州 510623
基金项目:
广东省科技厅项目（2015A030401075）.

摘要: 建立了QuEChERS-高效液相色谱-串联质谱（HPLC-MS/MS）同时测定猪肉、鱼肉、肝脏和肾脏中氯丙嗪、地西泮和安眠酮残留量的分析方法。样品用无水Na₂SO₄脱水、乙酸乙酯提取和C₁₈、N-丙基乙二胺（PSA）和氨基填料（NH₂填料）净化，使用一种特殊的C₁₈色谱柱Atlantis T3，以5 mmol/L甲酸溶液和乙腈溶液为流动相进行梯度洗脱。在正离子电离多反应监测（MRM）模式下，采用同位素内标法进行定量分析。3种镇静剂在0.2~5.0 μ g/L范围内线性关系良好。3种镇静剂在4类基质中3个水平（0.5、1和5 μ g/kg）下的加标回收率为92.5%~117.8%，相对标准偏差（RSD）为0.7%~11.6%（n=6）。该法高效快捷，灵敏度高，基质适应范围广，适用于大批量样品的快速分析。

关键词:

English

Determination of three tranquillizer residues in animal foods by QuEChERS-high performance liquid chromatography-tandem mass spectrometry

Inspection and Quarantine Technology Center, Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou 510623, China
Received Date: 30 March 2017
Fund Project:
Project of Guangdong Provincial Department of Science and Technology (No. 2015A030401075).

Abstract: A method for simultaneous determination of chlorpromazine, diazepam and metolazone residues in porcine muscle, fish, liver and kidney was developed using QuEChERS and HPLC-MS/MS technique. The samples were extracted with ethyl acetate and cleaned up with C₁₈, N-propylethylendiamine (PSA) and NH₂ sorbents after using Na₂SO₄ as dehydrating agent. The analytes were separated by a special C₁₈ column, Atlantis T3, and gradiently eluted with a mixed solution of 5 mmol/L formic acid and acetonitrile at a flow rate of 0.35 mL/min. The mass spectrometric analysis that quantified using isotope internal standard, was carried out with electrospray positive ion source (ESI⁺) and multiple reaction monitoring mode (MRM). The linearity of the calibration curves was good in the range of 0.2-5.0 μ g/L. The recoveries at three different spiked levels (0.5, 1 and 5 μ g/kg) in four matrices were in the range of 92.5%-117.8%. The repeatability expressed as relative standard deviations (RSDs) ranged from 0.7% to 11.6% (n=6). The method, with wide matrix range of application, is highly effective and sensitive and suitable for the rapid analysis of large quantities of samples.

Key words:

QuEChERS
/ high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)
/ tranquillizers
/ animal foods

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QuEChERS-高效液相色谱-串联质谱法测定动物源性食品中3种镇静剂残留

English

Determination of three tranquillizer residues in animal foods by QuEChERS-high performance liquid chromatography-tandem mass spectrometry

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

QuEChERS-高效液相色谱-串联质谱法测定动物源性食品中3种镇静剂残留

English

Determination of three tranquillizer residues in animal foods by QuEChERS-high performance liquid chromatography-tandem mass spectrometry

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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