Poly(N-isopropylacrylamide)-grafted Dual Stimuli-responsive Filter Paper for Protein Separation

Citation: Qi-jia-yu Wu, Rui Wang, Ying Zhou, Ya-qin Huang, Raja Ghosh, Xiao-nong Chen. Poly(N-isopropylacrylamide)-grafted Dual Stimuli-responsive Filter Paper for Protein Separation[J]. Chinese Journal of Polymer Science, 2015, 33(7): 1048-1057. doi: 10.1007/s10118-015-1655-6 shu

Poly(N-isopropylacrylamide)-grafted Dual Stimuli-responsive Filter Paper for Protein Separation

1.
1. Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, China
2.
2. Department of Chemical Engineering, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4L7, Canada
3.
4.
Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, China
5.
Department of Chemical Engineering, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4L7, Canada
基金项目:
This work was financially supported by the China Scholarship Council and the National Natural Science Foundation of China (No. 20874004).

摘要: Thermal and salt dual stimuli-responsive filter-paper-based membranes were prepared by UV-induced grafting of NIPAM-based polymers on paper surface. The grafting ratio could be controlled by monomer concentration during grafting polymerization. The results from pressure drop measurement of the mobile phase flowed cross the membrane demonstrate that an appropriate grafting ratio would be 8%-10%. Protein adsorption on the membrane through hydrophobic interaction could be promoted by increasing temperature and lyotropic salt concentration. The effect of grafted polymer structure on protein binding performance was studied. Filter paper grafted with NIPAM-based branched copolymer consisting of hydrophobic monomer moieties shows ten times higher protein binding capacity than that of the original filter paper. The separation of plasma proteins using the dual stimuli-responsive membrane was examined to demonstrate feasible application for hydrophobic interaction chromatographic separation of proteins.

关键词:

N-isopropyl acrylamide
/ Filter paper
/ Surface grafting
/ Stimuli-responsive hydrophobic transition
/ Protein separation

English

Poly(N-isopropylacrylamide)-grafted Dual Stimuli-responsive Filter Paper for Protein Separation

1.
1. Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, China
2.
2. Department of Chemical Engineering, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4L7, Canada
3.
4.
Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, China
5.
Department of Chemical Engineering, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4L7, Canada
Received Date: 06 November 2014
Revised Date: 20 December 2014
Available Online: 05 July 2015
Fund Project:
This work was financially supported by the China Scholarship Council and the National Natural Science Foundation of China (No. 20874004).

Abstract: Thermal and salt dual stimuli-responsive filter-paper-based membranes were prepared by UV-induced grafting of NIPAM-based polymers on paper surface. The grafting ratio could be controlled by monomer concentration during grafting polymerization. The results from pressure drop measurement of the mobile phase flowed cross the membrane demonstrate that an appropriate grafting ratio would be 8%-10%. Protein adsorption on the membrane through hydrophobic interaction could be promoted by increasing temperature and lyotropic salt concentration. The effect of grafted polymer structure on protein binding performance was studied. Filter paper grafted with NIPAM-based branched copolymer consisting of hydrophobic monomer moieties shows ten times higher protein binding capacity than that of the original filter paper. The separation of plasma proteins using the dual stimuli-responsive membrane was examined to demonstrate feasible application for hydrophobic interaction chromatographic separation of proteins.

Key words:

N-isopropyl acrylamide
/ Filter paper
/ Surface grafting
/ Stimuli-responsive hydrophobic transition
/ Protein separation

1. [1]
  Fei, Z., Wan, L., Wang, W., Zhong, M. and Xu, Z., J. Membr. Sci., 2013, 432: 42
2. [2]
  Chen, Y., Xie, R. and Chu, L., J. Membr. Sci., 2013, 442: 206
3. [3]
  Nunes, S.P., Behzad, A.R., Hooghan, B., Sougrat, R., Karunakaran, M., Pradeep, N., Vainio, U. and Peinemann, K., ACS Nano, 2011, 5: 3516
4. [4]
  Hoare, T., Timko, B.P., Santamaria, J., Goya, G.F., Irusta, S., Lau, S., Stefanescu, C.F., Lin, D., Langer, R. and Kohane, D.S., Nano Lett., 2011, 11: 1395
5. [5]
  Mah, K.Z. and Ghosh, R., J. Membr. Sci., 2010, 360: 149
6. [6]
  Okamura, A., Itayagoshi, M., Hagiwara, T., Yamaguchi, M., Kanamori, T., Shinbo, T. and Wang, P.C., Biomaterials, 2005, 26: 1287
7. [7]
  Huang, R., Mah, K.Z., Malta, M., Kostanski, L.K., Filipe, C.D.M. and Ghosh, R., J. Membr. Sci., 2009, 345: 177
8. [8]
  Song, F., Wang, X. and Wang, Y., Eur. Polym. J., 2011, 47: 1885
9. [9]
  Tokarev, I. and Minko, S., Adv. Mater., 2010, 22: 3446
10. [10]
  Vertommen, M.A.M.E., Cornelissen, H.L., Dietz, C.H.J.T., Hoogenboom, R., Kemmere, M.F. and Keurentjes, J.T.F., J. Membr. Sci., 2008, 322: 243
11. [11]
  Ozeki, S., Kurashima, H., Miyanaga, M. and Nozawa, C., Langmuir, 2000, 16: 1478
12. [12]
  Wanderaa, D., Wickramasinghe, S.R. and Husson, S.M., J. Membr. Sci., 2010, 357: 6
13. [13]
  Biazar, E., Khorasani, M.T. and Joupari, M.D., Chinese J. Polym. Sci., 2013, 31(11): 1509
14. [14]
  Zhao, Y., Zhao, H., Chen, L., Feng, X., Zhang, Q., Wang, J. and Zhan, R., J. Polym. Res., 2013, 20: 58
15. [15]
  Wan, P.J., Tan, D.S., Li, Z.S., Zhang, X.Q., Li, J.H. and Tan, H., Chinese J. Polym. Sci., 2012, 30(2): 190
16. [16]
  Kuckling, D., Colloid. Polym. Sci., 2009, 287: 881
17. [17]
  Yu, J., Zhu, L., Zhu, B. and Xu, Y., J. Membr. Sci., 2011, 366: 176
18. [18]
  Sun, T., Zhang, Y.F., Chen, C.L., Gong, X.Z. and Meng, J.Q., Chinese J. Polym. Sci, 2014, 32(7): 880
19. [19]
  Li, J.H., Miao, J., Shao, X.S., Xu, Y.Y. and Zhang, Q.Q., Chinese J. Polym. Sci., 2013, 31(7): 994
20. [20]
  Hsu, C., Wu, C. and Liu, Y., J. Membr. Sci., 2014, 450: 257
21. [21]
  Zhang, Y., Furyk, S., Bergbreiter, D.E. and Cremer, P.S., J. Am. Chem. Soc., 2005, 127: 14505
22. [22]
  Roettger, B.F. and Ladisch, M.R., Biotech. Adv., 1989, 7: 15
23. [23]
  Zhang, R., Yang, G., Xin, P., Qi, L. and Chen, Y., J. Chromatogr. A, 2009, 1216: 2404
24. [24]
  Yu, D., Chen, X., Pelton, R. and Ghosh, R., Biotech. Bioeng., 2008, 99: 1434
25. [25]
  Yang, L. and Chen, P., J. Membr. Sci., 2002, 205: 141
26. [26]
  Wu, Q., Wang, R., Chen, X. and Ghosh, R., J. Membr. Sci., 2014, 471: 56
27. [27]
  Ghosh, R. and Wong, T., J. Membr. Sci., 2006, 281: 532
28. [28]
  Laemmli, U.K., Nature, 1970, 227: 680
29. [29]
  Roy, D., Guthrie, J.T. and Perrier, S., Macromolecules, 2005, 38: 10363
30. [30]
  To, B.C.S. and Lenhoff, A.M., J. Chromatogr. A, 2007, 1141: 191

扫一扫看文章

计量

PDF下载量: 0
文章访问数: 872
HTML全文浏览量: 35

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Poly(N-isopropylacrylamide)-grafted Dual Stimuli-responsive Filter Paper for Protein Separation

English

Poly(N-isopropylacrylamide)-grafted Dual Stimuli-responsive Filter Paper for Protein Separation

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

Poly(N-isopropylacrylamide)-grafted Dual Stimuli-responsive Filter Paper for Protein Separation

English

Poly(N-isopropylacrylamide)-grafted Dual Stimuli-responsive Filter Paper for Protein Separation

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

文章相关

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs