超高效液相色谱-四极杆/静电场轨道阱高分辨质谱法测定焙烤食品及其原料中11种真菌毒素

引用本文: 李蓉, 何春梅, 杨璐齐, 王勇, 张朋杰, 高永清. 超高效液相色谱-四极杆/静电场轨道阱高分辨质谱法测定焙烤食品及其原料中11种真菌毒素[J]. 色谱, 2017, 35(8): 808-815. doi: 10.3724/SP.J.1123.2017.03035 shu

Citation: LI Rong, HE Chunmei, YANG Luqi, WANG Yong, ZHANG Pengjie, GAO Yongqing. Determination of 11 mycotoxins in baked foods and raw materials by ultra performance liquid chromatography-quadrupole/electrostatic field orbitrap high-resolution mass spectrometry[J]. Chinese Journal of Chromatography, 2017, 35(8): 808-815. doi: 10.3724/SP.J.1123.2017.03035 shu

超高效液相色谱-四极杆/静电场轨道阱高分辨质谱法测定焙烤食品及其原料中11种真菌毒素

1.
中山出入境检验检疫局, 广东中山 528400;
2.
广东药科大学食品科学学院, 广东中山 528458
基金项目:
广东省省级科技计划项目（2014A040401011）；国家质检总局科技计划项目（2015IK260）；中山市社会公益重大专项（2015B2295）.

摘要: 建立了超高效液相色谱-四极杆/静电场轨道阱高分辨质谱（UPLC-HRMS）测定焙烤食品及其原料中11种真菌毒素的检测分析方法。样品经20 mL含1%（体积分数）甲酸的乙腈-水（9：1，v/v）溶液提取，经2.0 g无水硫酸、0.5 g氯化钠和300 mg C18盐析、净化后进行检测。采用CORTECS C18色谱柱（100 mm×2.1 mm，1.6 μ m），以含0.1%（体积分数）甲酸的2 mmol/L乙酸铵溶液和含0.1%（体积分数）甲酸的2 mmol/L乙酸铵甲醇溶液为流动相进行梯度洗脱。结果显示，11种真菌毒素在各自的质量浓度范围内线性关系良好（相关系数r²≥0.9960），方法的定量限为0.15~20.00 μ g/kg，样品加标回收率为64.38%~122.61%，相对标准偏差为1.52%~12.99%（n=6）。该方法简单快速、灵敏度高、结果准确、可靠，利用该方法可有效测定焙烤食品及其原料中常见真菌毒素的含量。

关键词:

English

Determination of 11 mycotoxins in baked foods and raw materials by ultra performance liquid chromatography-quadrupole/electrostatic field orbitrap high-resolution mass spectrometry

1.
Zhongshan Entry-Exit Inspection and Quarantine Bureau, Zhongshan 528400, China;
2.
Guangdong Pharmaceutical University, Zhongshan 528458, China
Received Date: 27 March 2017
Fund Project:
Science and Technology Planning Project of Guangdong Province of China (No. 2014A040401011)

Abstract: A method for the determination of 11 mycotoxins in baked foods and raw materials by ultra-high performance liquid chromatography-quadrupole/electrostatic field orbitrap high-resolution mass spectrometry (UPLC-HRMS) is reported in this paper. The samples were extracted with 20 mL 90% (v/v) acetonitrile aqueous solution containing 1% (v/v) formic acid, and the extracts were salted out by 2.0 g MgSO₄ and 0.5 g NaCl, cleaned up by 300 mg C18. The analytes were carried out on a CORTECS C18 column (100 mm×2.1 mm, 1.6 μ m) by gradient elution with 2 mmol/L ammonium acetate with 0.1% (v/v) formic acid aqueous solution and 2 mmol/L ammonium acetate methanol with 0.1% (v/v) formic acid. The results showed that the 11 mycotoxins had good linear relationships in their respective mass concentration ranges. The correlation coefficients were not less than 0.9960 and the limits of quantitation (LOQs) were from 0.15 to 20.00 μ g/kg. The recoveries of the 11 mycotoxins in bread ranged from 64.38% to 122.61% with the relative standard deviations (RSDs) from 1.52% to 12.99% at three spiked levels (n=6). The method is demonstrated to be simple, fast, highly sensitive, reliable and it is effective to detect common mycotoxins in baked foods and raw materials.

Key words:

ultra-high performance liquid chromatography-high-resolution mass spectrometry (UPLC-HRMS)
/ mycotoxins
/ baked foods

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超高效液相色谱-四极杆/静电场轨道阱高分辨质谱法测定焙烤食品及其原料中11种真菌毒素

English

Determination of 11 mycotoxins in baked foods and raw materials by ultra performance liquid chromatography-quadrupole/electrostatic field orbitrap high-resolution mass spectrometry

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

超高效液相色谱-四极杆/静电场轨道阱高分辨质谱法测定焙烤食品及其原料中11种真菌毒素

English

Determination of 11 mycotoxins in baked foods and raw materials by ultra performance liquid chromatography-quadrupole/electrostatic field orbitrap high-resolution mass spectrometry

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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