基于钒酸铈纳米酶检测葡萄糖和总抗氧化能力的研究

引用本文: 阮栎婷, 何绍英, 赵婉, 曹红帅, 徐志爱, 张文. 基于钒酸铈纳米酶检测葡萄糖和总抗氧化能力的研究[J]. 分析化学, 2022, 50(9): 1319-1327. doi: 10.19756/j.issn.0253-3820.221099 shu

Citation: RUAN Li-Ting, HE Shao-Ying, ZHAO Wan, CAO Hong-Shuai, XU Zhi-Ai, ZHANG Wen. Cerium Vanadate-based Peroxidase-like Nanozyme for Glucose and Total Antioxidant Capacity Assay[J]. Chinese Journal of Analytical Chemistry, 2022, 50(9): 1319-1327. doi: 10.19756/j.issn.0253-3820.221099 shu

基于钒酸铈纳米酶检测葡萄糖和总抗氧化能力的研究

华东师范大学化学与分子工程学院, 上海 200241

通讯作者: 张文,E-mail:wzhang@chem.ecnu.edu.cn

基金项目:
国家自然科学基金项目（No.22174047）资助。

摘要: 以精氨酸(Arg)取代乙二胺四乙酸(EDTA)作为稳定剂,采用水热法合成了钒酸铈(CeVO₄)纳米酶。稳定剂的改变使得CeVO₄纳米材料的形貌从纳米棒转变为纳米粒子,同时,CeVO₄中Ce^Ⅲ和Ce^Ⅳ比值改变使得纳米酶活性由较强的类超氧化物歧化酶(SOD)转变成较强的类过氧化物酶(POD)。在3,3',5,5'-四甲基联苯胺(TMB)和过氧化氢(H₂O₂)反应体系中,CeVO₄纳米粒子催化H₂O₂产生羟基自由基(·OH),·OH氧化TMB,使溶液由无色变为蓝色,证明其具有较强的类POD活性。基于CeVO₄纳米酶的类POD活性,建立了检测H₂O₂和葡萄糖的比色传感平台,H₂O₂和葡萄糖响应的线性范围分别为10~1500 μmol/L和10~120 μmol/L,检出限(3σ)分别为1.1 μmol/L和3.3 μmol/L。将本方法应用于实际样品中总抗氧化能力(TAC)分析,结果令人满意。

关键词:

English

Cerium Vanadate-based Peroxidase-like Nanozyme for Glucose and Total Antioxidant Capacity Assay

School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, China

Corresponding author: ZHANG Wen, wzhang@chem.ecnu.edu.cn

Received Date: 24 February 2022
Revised Date: 13 April 2022
Fund Project:
Supported by the National Natural Science Foundation of China (No.22174047).

Abstract: The cerium vanadate (CeVO₄)-based nanozyme was synthesized through a hydrothermal synthesis method with arginine as the stabilizer. Due to the change of stabilizer, the ratio of Ce^Ⅲ to Ce^Ⅳ in CeVO₄ changed and thus allowed that the nanozyme showed nanoparticle morphology, which was different from that of nanorod prepared with EDTA as stabilizer. In the reaction system of 3,3',5,5'-tetramethylbenzidine (TMB) and hydrogen peroxide (H₂O₂), the CeVO₄-based nanozyme first catalyzed H₂O₂ to produce hydroxyl radical (·OH), which could oxidize TMB and thus resulted in a colour change of solution from colourless to blue, proving that CeVO₄ prepared with arginine had strong peroxidase-like activity and the generation of ·OH accounted for the outstanding peroxidase-like properties. Based on these features, a colorimetric method was developed for detecting H₂O₂, glucose and antioxidants. This study not only provided a new direction for the research of CeVO₄-based nanozyme, but also developed a fast and simple method for total antioxidant capacity (TAC) assay.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

基于钒酸铈纳米酶检测葡萄糖和总抗氧化能力的研究

通讯作者: 张文,E-mail:wzhang@chem.ecnu.edu.cn

English

Cerium Vanadate-based Peroxidase-like Nanozyme for Glucose and Total Antioxidant Capacity Assay

Corresponding author: ZHANG Wen, wzhang@chem.ecnu.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于钒酸铈纳米酶检测葡萄糖和总抗氧化能力的研究

通讯作者: 张文,E-mail:wzhang@chem.ecnu.edu.cn

English

Cerium Vanadate-based Peroxidase-like Nanozyme for Glucose and Total Antioxidant Capacity Assay

Corresponding author: ZHANG Wen, wzhang@chem.ecnu.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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