引用本文:
Ze Yi YAN, Cheng Jin JIAO, Feng Min LI, Yong Min LIANG, Zhi Xiao LI. HPLC Determination of the Major Non-protein Amino Acids and Common Biogenic Amines in Lathyrus sativus Using a Novel Extraction Method[J]. Chinese Chemical Letters,
2005, 16(5): 639-642.
Citation: Ze Yi YAN, Cheng Jin JIAO, Feng Min LI, Yong Min LIANG, Zhi Xiao LI. HPLC Determination of the Major Non-protein Amino Acids and Common Biogenic Amines in Lathyrus sativus Using a Novel Extraction Method[J]. Chinese Chemical Letters, 2005, 16(5): 639-642.
Citation: Ze Yi YAN, Cheng Jin JIAO, Feng Min LI, Yong Min LIANG, Zhi Xiao LI. HPLC Determination of the Major Non-protein Amino Acids and Common Biogenic Amines in Lathyrus sativus Using a Novel Extraction Method[J]. Chinese Chemical Letters, 2005, 16(5): 639-642.
HPLC Determination of the Major Non-protein Amino Acids and Common Biogenic Amines in Lathyrus sativus Using a Novel Extraction Method
摘要:
An assay is presented for simultaneously determining 5 biogenic amines and the major non-protein amino acids:the toxin β-N-oxalyl-L-α,β-diaminopropanoic acid (β-ODAP), its isomer α-ODAP and homoarginine in Lathyrus sativus extracts using the HPLC system after derivatization with para-nitrobenzyloxycarbonyl chloride (PNZ-C1). However, it is more worthy of noting that this paper also describes a new extraction method using 0.2 mol/L HC1O4. The new method has some advantages:shorter extraction-time, simultaneous extraction of free amino acids and polyamines, better inhibiting the isomerization of β-ODAP to α-ODAP, and so on.
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关键词:
- ODAP
- / homoginine
- / HPLC
- / biogenic amines
- / new extraction method
English
HPLC Determination of the Major Non-protein Amino Acids and Common Biogenic Amines in Lathyrus sativus Using a Novel Extraction Method
Abstract:
An assay is presented for simultaneously determining 5 biogenic amines and the major non-protein amino acids:the toxin β-N-oxalyl-L-α,β-diaminopropanoic acid (β-ODAP), its isomer α-ODAP and homoarginine in Lathyrus sativus extracts using the HPLC system after derivatization with para-nitrobenzyloxycarbonyl chloride (PNZ-C1). However, it is more worthy of noting that this paper also describes a new extraction method using 0.2 mol/L HC1O4. The new method has some advantages:shorter extraction-time, simultaneous extraction of free amino acids and polyamines, better inhibiting the isomerization of β-ODAP to α-ODAP, and so on.
-
Key words:
- ODAP
- / homoginine
- / HPLC
- / biogenic amines
- / new extraction method
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