固相萃取-高效液相色谱测定饲料中新橙皮苷二氢查耳酮和柚皮苷二氢查耳酮

引用本文: 潘城, 胡朝阳, 任小英, 吴凌, 黄何何, 江凤玲, 谢勇, 邱秀玉. 固相萃取-高效液相色谱测定饲料中新橙皮苷二氢查耳酮和柚皮苷二氢查耳酮[J]. 色谱, 2019, 37(6): 649-654. doi: 10.3724/SP.J.1123.2019.01006 shu

Citation: PAN Cheng, HU Chaoyang, REN Xiaoying, WU Ling, HUANG Hehe, JIANG Fengling, XIE Yong, QIU Xiuyu. Determination of neohesperidin dihydrochalcone and naringin dihydrochalcone in feeds by solid phase extraction-high performance liquid chromatography[J]. Chinese Journal of Chromatography, 2019, 37(6): 649-654. doi: 10.3724/SP.J.1123.2019.01006 shu

固相萃取-高效液相色谱测定饲料中新橙皮苷二氢查耳酮和柚皮苷二氢查耳酮

福建省产品质量检验研究院, 福建福州 350002

摘要: 建立了固相萃取-高效液相色谱检测饲料中新橙皮苷二氢查耳酮（NHDC）和柚皮苷二氢查耳酮（Naringin DC）的方法。采用纯甲醇溶液进行超声辅助萃取样品，经HLB固相萃取柱净化后在XB-C₁₈（150 mm×4.6 mm，5 μm）色谱柱上分离，用甲醇/水作为流动相进行梯度洗脱，光电二极管阵列检测器进行检测。结果表明，新橙皮苷二氢查耳酮和柚皮苷二氢查耳酮在0.2~49.0 mg/L范围内具有良好的线性关系，相关系数均>0.999，定量限分别为0.02和0.01 mg/kg。日内和日间精密度分别为0.7%~4.1%和0.9%~6.0%。方法的加标回收率为86.2%~105.0%，相对标准偏差（RSDs）为1.0%~6.3%（n=3）。该方法能有效降低饲料基体成分的干扰，灵敏度高，重现性好，适用于饲料中新橙皮苷二氢查耳酮和柚皮苷二氢查耳酮的定量检测。

关键词:

English

Determination of neohesperidin dihydrochalcone and naringin dihydrochalcone in feeds by solid phase extraction-high performance liquid chromatography

Fujian Inspection and Research Institute for Product Quality, Fuzhou 350002, China
Received Date: 06 January 2019

Abstract: A new method was established for the determination of neohesperidin dihydrochalcone (NHDC) and naringin dihydrochalcone (Naringin DC) in feeds by solid phase extraction-high performance liquid chromatography (SPE-HPLC). The samples were extracted by methanol and were purified on an HLB solid-phase extraction column. Chromatographic separation was achieved on an XB-C₁₈ column (150 mm×4.6 mm, 5 μm) by linear gradient elution using methanol/water as the mobile phase. The analytes were detected by the diode array detector (DAD). The results revealed a good linear correlation (r>0.999) between the peak areas and mass concentrations of dihydrochalcone sweeteners in the range of 0.2-49.0 mg/L. The limits of quantification (LOQs) of NHDC and Naringin DC were 0.02 and 0.01 mg/kg, respectively. Intra- and inter-day reproducibilities were 0.7%-4.1% and 0.9%-6.0%, respectively. The spiked recoveries for the samples and relative standard deviations (RSDs) were 86.2%-105.0% and 1.0%-6.3% (n=3), respectively. It is both sensitive and repeatable for the quantitative determination of neohesperidin dihydrochalcone and naringin dihydrochalcone in feeds, and thus, can be used to effectively reduce interference in feeds.

Key words:

solid phase extraction (SPE)
/ high performance liquid chromatography (HPLC)
/ neohesperidin dihydrochalcone (NHDC)
/ naringin dihydrochalcone (Naringin DC)
/ feeds

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沈阳化工大学材料科学与工程学院沈阳 110142

固相萃取-高效液相色谱测定饲料中新橙皮苷二氢查耳酮和柚皮苷二氢查耳酮

English

Determination of neohesperidin dihydrochalcone and naringin dihydrochalcone in feeds by solid phase extraction-high performance liquid chromatography

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

固相萃取-高效液相色谱测定饲料中新橙皮苷二氢查耳酮和柚皮苷二氢查耳酮

English

Determination of neohesperidin dihydrochalcone and naringin dihydrochalcone in feeds by solid phase extraction-high performance liquid chromatography

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

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CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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