核酸荧光探针在单细胞成像中的应用研究

引用本文: 贾永梅, 娄筱叮, 夏帆. 核酸荧光探针在单细胞成像中的应用研究[J]. 分析化学, 2018, 46(9): 1329-1338. doi: 10.11895/j.issn.0253-3820.181340 shu

Citation: JIA Yong-Mei, LOU Xiao-Ding, XIA Fan. Fluorescent Bioprobe Based on Nucleic Acid for Live Cell Imaging[J]. Chinese Journal of Analytical Chemistry, 2018, 46(9): 1329-1338. doi: 10.11895/j.issn.0253-3820.181340 shu

核酸荧光探针在单细胞成像中的应用研究

1.
岭南师范学院化学化工学院, 湛江 524048;
2.
中国地质大学(武汉)材料与化学学院, 武汉 430074;
3.
华中科技大学化学与化工学院, 武汉 430074
基金项目:
本文系国家自然科学基金项目（No.21705071）、广东省自然科学基金项目（No.2016A030310362）和岭南师范学院自然科学引进人才项目（No.ZL1611）资助

摘要: 单细胞成像研究技术具有观测定位精准、独特的时间和空间分辨率高等特点，成为分子水平上准确、实时、原位监测细胞内生物活性物质信号变化及观测细胞形态的重要手段。在进行单细胞成像研究时，细胞内大多数生物活性物质自身无法产生易被仪器或肉眼所捕获的信号，通常需要使用生物探针进入细胞内特异性识别生物活性物质。生物探针与特定的生物活性物质结合，形成稳定的复合物，产生相应信号变化。通过监测生物探针信号变化，实现对细胞内生物活性物质准确、实时、原位监测。生物探针的响应信号有多种，其中荧光信号不仅具有直观、操作简单、选择性好、灵敏度高、无需参比、不受电磁场的影响、可远程实时在线自动监测等特性，还具备对细胞进行静态观察、对细胞内分子动力学进行动态监测、对亚细胞结构进行定位和对蛋白质分子的相互作用进行研究等优点，在细胞成像技术研究中具有重要地位。本文综述了近五年来核酸荧光探针在单细胞成像中的应用研究进展，讨论了存在的问题，对发展方向进行了展望。

关键词:

English

Fluorescent Bioprobe Based on Nucleic Acid for Live Cell Imaging

1.
School of Chemistry and Chemical Engineering, Lingnan Normal University, Zhanjiang 524048, China;
2.
Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan 430074, China;
3.
School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China
Received Date: 21 May 2018
Revised Date: 11 July 2018
Fund Project:
This work was supported by the National Natural Science Foundation of China (No. 201705071), the Natural Science Foundation of Gugangdong Province, China (No. 2016A030310362), and the Initiatory Financial Support from Lingnan Normal University (No. ZL1611).

Abstract: Live cell imaging is an essential tool for detecting intracellular biomolecules, particularly those that are key targets in disease processes and monitoring their functions in live cells. Usually, the intracellular biomolecules cannot make out signal change that could be detected by instrument or naked eye directly. Thus, target identification and validation with high-affinity probes is one of the key prerequisites for interrogation of specific molecular targets in living systems. There are many kinds of bioprobes. Among them, fluorescent bioprobes based on nucleic acids are essential tools due to their high sensitivity, fast response, simplicity, real-time, on-site and non-invasive visualization of biological molecules and processes in live cells. They not only provide valuable insight in understanding physiological alterations in pathological settings but also serve as useful tools for the development of personalized medicine and targeted therapies. This review summarizes the progress of fluorescent bioprobes based on nucleic acids for live cell imaging in recent 5 years.

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

核酸荧光探针在单细胞成像中的应用研究

English

Fluorescent Bioprobe Based on Nucleic Acid for Live Cell Imaging

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

核酸荧光探针在单细胞成像中的应用研究

English

Fluorescent Bioprobe Based on Nucleic Acid for Live Cell Imaging

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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