QuEChERS-超高效液相色谱-串联质谱法测定食用菌中7种荧光增白剂

引用本文: 张宪臣, 李蓉, 周艳萍, 薄艳娜, 王京力, 胡仪光. QuEChERS-超高效液相色谱-串联质谱法测定食用菌中7种荧光增白剂[J]. 分析化学, 2017, 45(5): 777-783. doi: 10.11895/j.issn.0253-3820.160866 shu

Citation: ZHANG Xian-Chen, LI Rong, ZHOU Yan-Ping, BO Yan-Na, WANG Jing-Li, HU Yi-Guang. Simultaneous Determination of Seven Kinds of Fluorescent White Agents in Mushroom by QuEChERS-Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, 2017, 45(5): 777-783. doi: 10.11895/j.issn.0253-3820.160866 shu

QuEChERS-超高效液相色谱-串联质谱法测定食用菌中7种荧光增白剂

张宪臣 ^1, ,
李蓉 ^1, ,
周艳萍 ^1, ,
薄艳娜 ^2, ,
王京力 ^1, ,
胡仪光 ^1,

1.
广东出入境检验检疫局检验检疫技术中心中山分中心, 中山 528403;
2.
暨南大学包装工程研究所, 广东普通高校产品包装与物流重点实验室, 珠海 519070
基金项目:
本文系国家质检总局科技计划项目（2015IK052，2015IK260）、广东省科技计划项目（2014A040401011）、中山市社会公益重大专项（2015B2295，2014A2FC241）、广东检验检疫局科技计划项目（2015GDK32，2017GDK24）资助

摘要: 建立了QuEChERS-超高效液相色谱-串联质谱法（UPLC-MS/MS）测定食用菌中7种荧光增白剂残留量的检测方法。样品前处理采用QuEChERS方法，食用菌样品用水浸润后，以甲酸和乙腈提取目标物，C₁₈填料、正丙基乙二胺（PSA）和MgSO₄净化，C₁₈色谱柱分离，乙腈和0.1%甲酸梯度洗脱，多反应监测（MRM）正离子模式扫描。在优化条件下，7种荧光增白剂在一定质量浓度范围内线性关系良好，相关系数均大于0.991，方法检出限（S/N=3）在0.05-0.40 μg/kg之间，定量低限（S/N≥10）在0.2-1.3 μg/kg之间，方法的平均回收率在70.1%-109.2%之间。本方法具有操作简单快捷、灵敏度高等优点。

关键词:

English

Simultaneous Determination of Seven Kinds of Fluorescent White Agents in Mushroom by QuEChERS-Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

1.
Zhongshan Subcenter of Inspection and Quarantine Technology Center, Guangdong Entry-Exit Inspection and Quarantine Bureau, Zhongshan 528403, China;
2.
Key Laboratory of Product Packaging and Logistics, Institute of Packaging Engineering of Guangdong Higher Education Institutes, Jinan University, Zhuhai 519070, China
Received Date: 27 November 2016
Revised Date: 20 March 2017

Abstract: A method based on QuEChERS coupled with ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed for the determination of seven kinds of fluorescent white agent residues in mushroom. After mixing with 10 mL of water, the sample was extracted with acidified acetonitrile, cleaned up by C₁₈, primary secondary amine (PSA) and MgSO₄. The separation of seven kinds of fluorescent white agents was performed on a C₁₈ column with a gradient elution of acetonitrile and 0.1% acidified water as mobile phase. The target compounds were detected by electrospray ionization mass spectrometry in positive ion mode with multi reaction monitoring (MRM). Under the optimum conditions, the method had good linear relationship in the determination of the seven kinds of fluorescent white agents in a certain concentration range, with correlation coefficients greater than 0.991. Moreover, the limits of detection (S/N=3) were 0.05-0.4 μg/kg, the limits of quantitation (S/N=10) were 0.2-1.3 μg/kg, and the average recoveries for seven kinds of fluorescent white agent residues in msushroom were 70.1%-109.2%. In comparison with previous methods, the new procedure has characteristics of simple sample preparation and higher sensitivity.

Key words:

Ultra performance liquid chromatography-tandem mass spectrometry
/ Fluorescent white agents
/ Mushroom

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沈阳化工大学材料科学与工程学院沈阳 110142

QuEChERS-超高效液相色谱-串联质谱法测定食用菌中7种荧光增白剂

English

Simultaneous Determination of Seven Kinds of Fluorescent White Agents in Mushroom by QuEChERS-Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

QuEChERS-超高效液相色谱-串联质谱法测定食用菌中7种荧光增白剂

English

Simultaneous Determination of Seven Kinds of Fluorescent White Agents in Mushroom by QuEChERS-Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

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