替代模板印迹-高效液相色谱测定空气PM2.5中的羰基化合物

引用本文: 方思炀, 陈诗丽, 何嘉铧, 王浩, 沈丽思, 刘洋洋, 孙慧, 赖家平. 替代模板印迹-高效液相色谱测定空气PM2.5中的羰基化合物[J]. 色谱, 2019, 37(6): 634-643. doi: 10.3724/SP.J.1123.2018.12012 shu

Citation: FANG Siyang, CHEN Shili, HE Jiahua, WANG Hao, SHEN Lisi, LIU Yangyang, SUN Hui, LAI Jiaping. Determination of carbonyls in ambient PM2.5 by coupling dummy template imprinting technology and high performance liquid chromatography[J]. Chinese Journal of Chromatography, 2019, 37(6): 634-643. doi: 10.3724/SP.J.1123.2018.12012 shu

替代模板印迹-高效液相色谱测定空气PM2.5中的羰基化合物

方思炀 ^1, ,
陈诗丽 ^1, ,
何嘉铧 ^1, ,
王浩 ^1, ,
沈丽思 ^1, ,
刘洋洋 ^1, ,
孙慧 ^1,3, ,
赖家平 ^2,

1.
广州大学环境科学与工程学院, 广东广州 510006;
2.
华南师范大学化学与环境学院, 广东广州 510006;
3.
广东省放射性核素污染控制以及资源化重点实验室, 广东广州 510006
基金项目:
国家自然科学基金（21477026，21677053，21876033）；广州市科技计划项目（201607010295）；广州大学大学生创新训练项目（201711078061）.

摘要: 以替代模板法制备了分子印迹固相萃取柱（MISPE），该柱能够有效去除空气PM2.5样品分析过程中过量的衍生剂2，4-二硝基苯肼（2，4-DNPH），并能够大幅度浓缩样品溶液，提高羰基化合物的富集效率和监测灵敏度，缩短采样时间。与高效液相色谱联用，实现了对空气PM2.5中的羰基化合物的快速检测。本研究解决了传统固相萃取材料选择性低，不能去除2，4-DNPH干扰的瓶颈问题。本方法用于研究广州市大学城早春季节雾霾天和非霾天羰基化合物水平及其来源，结果发现无论是灰霾天气还是正常天气，14种目标羰基化合物均被检出。研究发现，在污染物排放和天气因素的影响下，灰霾天气羰基化合物总量增高，尤其是异戊醛增长迅速，并且与丙醛浓度变化呈现高度正相关。通过相关性分析，证明除了光化学反应等自然因素，人类活动对广州羰基化合物水平的干扰严重，应引起重视。

关键词:

English

Determination of carbonyls in ambient PM2.5 by coupling dummy template imprinting technology and high performance liquid chromatography

1.
School of Environmental Science & Engineering, Guangzhou University, Guangzhou 510006, China;
2.
School of Chemistry & Environment, South China Normal University, Guangzhou 510006, China;
3.
Guangdong Provincial Key Laboratory of Radionuclides Pollution Control and Resources, Guangzhou 510006, China
Received Date: 09 December 2018
Fund Project:
National Natural Science Foundation of China (Nos. 21477026, 21677053, 21876033)

Abstract: The determination of ambient carbonyls by the derivative method inevitably leads to the introduction of extra 2,4-dinitrophenylhydrazine (2,4-DNPH) into the sample solution. Traditional solid phase extraction (SPE) materials cannot remove this interference. To address this issue, a selective molecularly imprinted solid phase extraction (MISPE) column was prepared. With 2,4-dinitroaniline (2,4-DNAN) as the dummy template, MISPE could eliminate the excessive derivative agent (2,4-DNPH) in the air samples. Consequently, the sample could be concentrated effectively while the sensitivity increased greatly. By coupling MISPE and high performance liquid chromatography (HPLC), the developed method was used to study the concentration and source of 14 carbonyls of PM2.5 during spring in Guangzhou Higher Education Mega Center. Results showed that the total concentration of carbonyls increased with the level of air pollution. Particularly, the content of isovaleraldehyde increased sharply to account for 21% of the 14 total carbonyls in the haze days. Moreover, a high positive correlation between isovaleraldehyde and propionaldehyde was found either in normal days or haze days. From the correlation analysis, it was shown that anthropogenic emissions together with photochemical reaction had contributed to the abnormally high levels of carbonyls in ambient PM2.5 in the haze days. This aspect should be further investigated.

Key words:

molecularly imprinted polymer (MIP)
/ solid phase extraction (SPE)
/ high performance liquid chromatography (HPLC)
/ carbonyls
/ PM2.5
/ air

1. [1] Fu Z R, Feng Y L, Ren Q Q, et al. China Environmental Science, 2013, 33(11):1937 付正茹, 冯艳丽, 任青青, 等. 中国环境科学, 2013, 33(11):1937
2. [2] Sun Y S, Qu Q S, Yu S X, et al. Chinese Journal of Chromatography, 2016, 34(12):1249 孙元社, 瞿其曙, 于淑新, 等. 色谱, 2016, 34(12):1249
3. [3] Zhang Y J, Mu Y J, Liu J F, et al. J Environ Sci, 2012, 24(1):124
4. [4] Cheng Y, Lee S C, Huang Y, et al. Atmos Environ, 2014, 89(2):43
5. [5] Swenberg J A, Moeller B C, Lu K, et al. Toxicol Pathol, 2013, 41(2):181
6. [6] Zhang Y, Zhao D, Wang B, et al. Instrum Sci Technol, 2015, 43(3):344
7. [7] Li Y, Sun H, Lai J, et al. Microchim Acta, 2018, 185(2):122
8. [8] Wang L J, Cao X M, Sun X L, et al. Chinese Journal of Chromatography, 2016, 34(3):240 王丽君, 曹旭敏, 孙晓亮, 等. 色谱, 2016, 34(3):240
9. [9] Liu J, Lu W H, Cui R, et al. Chinese Journal of Chromatography, 2018, 36(1):30 刘洁, 鹿文慧, 崔荣, 等. 色谱, 2018, 36(1):30
10. [10] Sun H, Lai J P, Fung Y S. J Chromatogr A, 2014, 1358(2):303
11. [11] Tian D T. Molecularly Imprinted Polymer Functional Material. Beijing:Science Press, 2017 田大听. 分子印迹聚合物功能材料. 北京:科学出版社, 2017
12. [12] Karim K, Breton F, Rouillon R, et al. Adv Drug Deliver Rev, 2005, 57 (12):1795
13. [13] Jiang C H, Wang Y J, Zheng X, et al. Research of Environmental Sciences, 2016, 29(9):1272 蒋朝晖, 王玉娇, 郑玄, 等. 环境科学研究, 2016, 29(9):1272
14. [14] Lü H X, Cai Q Y. Ecology and Environmental Sciences, 2009, 18(4):1533 吕辉雄, 蔡全英. 生态环境学报, 2009, 18(4):1533
15. [15] Andraca-Ayala G, Ruiz-Suarez L G. Atmosfera, 2005, 18(3):189
16. [16] Ochsenkhn K M, Lyberopoulou T, Koumarianou G, et al. Microchim Acta, 2007, 160(4):485
17. [17] Bao L F, Matsumoto M, Kubota T, et al. Atmos Environ, 2012, 47:546
18. [18] Organization W T. WHO Guidelines For Indoor Air Quality-Selected Pollutants. Geneva Switzerland:WHO Regional Office for Europe, 2010
19. [19] Ho S S H, Ip H S S, Ho K F, et al. J Air Waste Manage, 2013, 63(7):864

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替代模板印迹-高效液相色谱测定空气PM2.5中的羰基化合物

English

Determination of carbonyls in ambient PM2.5 by coupling dummy template imprinting technology and high performance liquid chromatography

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

替代模板印迹-高效液相色谱测定空气PM2.5中的羰基化合物

English

Determination of carbonyls in ambient PM2.5 by coupling dummy template imprinting technology and high performance liquid chromatography

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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