Determination of Kinetic Parameters and Metal Ions in Urea-Urease System Based on the Biochemical Reaction Heat Induced Laser Beam Deflection

Hong Tao YAN Xiao Yun ZHU

引用本文: Hong Tao YAN,  Xiao Yun ZHU. Determination of Kinetic Parameters and Metal Ions in Urea-Urease System Based on the Biochemical Reaction Heat Induced Laser Beam Deflection[J]. Chinese Chemical Letters, 2000, 12(2): 157-160. shu
Citation:  Hong Tao YAN,  Xiao Yun ZHU. Determination of Kinetic Parameters and Metal Ions in Urea-Urease System Based on the Biochemical Reaction Heat Induced Laser Beam Deflection[J]. Chinese Chemical Letters, 2000, 12(2): 157-160. shu

Determination of Kinetic Parameters and Metal Ions in Urea-Urease System Based on the Biochemical Reaction Heat Induced Laser Beam Deflection

摘要: A new analytical method for the determination of urea-urease system based on biochemical reaction heat induced laser beam deflection is presented in this paper.With the method, the Michaelis constant (Km) of urease and apparent inhibition constant (Ki) of some metal ion inhibitors were measured respectively.This method was also used for the quantitative determination of metal ions with satisfactory result.

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  • 收稿日期:  1999-06-15
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    沈阳化工大学材料科学与工程学院 沈阳 110142

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