凝集素亲和法在糖蛋白癌症生物标志物分析和检测中的应用

引用本文: 李萍, 许文辉, 张大伟, 贾琼. 凝集素亲和法在糖蛋白癌症生物标志物分析和检测中的应用[J]. 分析化学, 2021, 49(9): 1451-1460. doi: 10.19756/j.issn.0253-3820.210435 shu

Citation: LI Ping, XU Wen-Hui, ZHANG Da-Wei, JIA Qiong. Application of Lectin Affinity Methods in Analysis and Detection of Glycoprotein Cancer Biomarkers[J]. Chinese Journal of Analytical Chemistry, 2021, 49(9): 1451-1460. doi: 10.19756/j.issn.0253-3820.210435 shu

凝集素亲和法在糖蛋白癌症生物标志物分析和检测中的应用

吉林大学化学学院, 长春 130012

通讯作者: 张大伟,E-mail:zhangdw@jlu.edu.cn; 贾琼,E-mail:jiaqiong@jlu.edu.cn

基金项目:
吉林省科技厅自然科学基金项目（No.20190201079JC）资助。

摘要: 糖蛋白在调节生物体内各种生理过程中具有重要作用，异常的蛋白质糖基化通常与癌症等疾病的发生和发展有关，许多糖蛋白可作为癌症早期诊断或预后监测的生物标志物。在进行糖蛋白质谱分析鉴定前，从复杂生物样品中有效分离富集糖蛋白是必要的步骤。凝集素对某些特定的糖蛋白具有选择性，凝集素亲和法被广泛用于糖蛋白的分离富集。本文以4种发病率最高的癌症（乳腺癌、肺癌、结直肠癌、前列腺癌）为例，综述了凝集素亲和法与质谱结合在筛选和分析糖蛋白癌症生物标志物中的应用。

关键词:

凝集素
/ 糖蛋白
/ 癌症
/ 生物标志物
/ 评述

English

Application of Lectin Affinity Methods in Analysis and Detection of Glycoprotein Cancer Biomarkers

College of Chemistry, Jilin University, Changchun 130012, China

Corresponding author: ZHANG Da-Wei, ; JIA Qiong,

Received Date: 13 April 2021
Revised Date: 22 June 2021
Fund Project:
Supported by Natural Science Foundation of Science and Technology Department of Jilin Province (No.201902010079JC).

Abstract: Glycoproteins play an important role in regulating various physiological processes of organisms. Abnormal protein glycosylation is usually associated with the formation and evolution of diseases such as cancer. Many specific glycoproteins can be used as the biomarkers for early cancer diagnosis or prognosis monitoring. Before identifying the glycoproteins with mass spectrometry, effective separation and enrichment of glycoproteins from complex biological samples is a necessary step. Lectins are selective for certain specific glycoproteins, and lectin affinity methods have been widely used in the separation and enrichment of glycoprotein. By taking the four kinds of cancers (breast cancer, lung cancer, colorectal cancer and prostate cancer) with the highest incidence as examples, this article reviews the applications of lectin affinity methods integrated with mass spectrometry in screening and analyzing cancer biomarkers.

Key words:

Lectin
/ Glycoprotein
/ Cancer
/ Biomarker
/ Review

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沈阳化工大学材料科学与工程学院沈阳 110142

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

凝集素亲和法在糖蛋白癌症生物标志物分析和检测中的应用

通讯作者: 张大伟,E-mail:zhangdw@jlu.edu.cn; 贾琼,E-mail:jiaqiong@jlu.edu.cn

English

Application of Lectin Affinity Methods in Analysis and Detection of Glycoprotein Cancer Biomarkers

Corresponding author: ZHANG Da-Wei, ; JIA Qiong,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

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