基于计时库仑技术的可再生型三磷酸腺苷适配体电化学传感器的研究

引用本文: 卢莹, 田燕, 王莉, 杨耀, 姚晓林. 基于计时库仑技术的可再生型三磷酸腺苷适配体电化学传感器的研究[J]. 分析化学, 2017, 45(5): 721-726. doi: 10.11895/j.issn.0253-3820.160645 shu

Citation: LU Ying, TIAN Yan, WANG Li, YANG Yao, YAO Xiao-Lin. A Reusable Chronocoulometric Aptamer Sensor for Detection of Adenosine Triphosphate[J]. Chinese Journal of Analytical Chemistry, 2017, 45(5): 721-726. doi: 10.11895/j.issn.0253-3820.160645 shu

基于计时库仑技术的可再生型三磷酸腺苷适配体电化学传感器的研究

卢莹 ^1,2, ,
田燕 ^2, ,
王莉 ^2, ,
杨耀 ^2, ,
姚晓林 ^1,

1.
茶树生物学与资源利用国家重点实验室, 安徽农业大学, 合肥 230036;
2.
安徽农业大学理学院应用化学系, 安徽农业大学, 合肥 230036
基金项目:
本文系国家自然科学基金项目（No.21305002）和安徽农业大学人才项目（No. yj2015-28）资助

摘要: 构建了一种可再生型三磷酸腺苷（ATP）适配体计时库仑电化学传感器。将一条短链DNA通过AuS键自组装固定在电极表面，ATP的核酸适配体与该短链DNA杂交而结合在电极表面。带负电的DNA通过静电吸引结合电解液中的六氨合钌（RuHex）阳离子。当传感器和靶分子ATP孵育后，ATP与核酸适配体结合，使适配体链从电极表面解离，电极表面吸附的DNA量减少，结合RuHex的量随之降低。通过计时库仑技术检测RuHex响应信号降低的量，可以对ATP进行定量测定。此传感器的电化学响应信号与ATP浓度对数值呈线性关系，线性检测范围为0.001~100 μmol/L，检出限（S/N=3）为0.5 nmol/L。此传感器检测靶分子ATP后，可以通过简单的操作步骤再生，再生5次后的响应信号为初始信号的90%以上。采用此传感器检测大鼠脑透析液中ATP的含量为（19.2±3.7） nmol/L （n=3）。

关键词:

English

A Reusable Chronocoulometric Aptamer Sensor for Detection of Adenosine Triphosphate

1.
State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, China;
2.
Department of Applied Chemistry, School of Natural Sciences, Anhui Agricultural University, Hefei 230036, China
Received Date: 30 August 2016
Revised Date: 06 March 2017
Fund Project:
This work was supported by the National Natural Science Foundation of China (No.21305002)

Abstract: A reusable chronocoulometric adenosine triphosphate (ATP)-aptamer sensor was developed in this work. A short chain of DNA marked as cDNA containing complementary sequence was immobilized on gold electrode based on Au-S self-assembly. The ATP aptamer was hybridized with cDNA. The surface-confined DNA could bind with ［Ru(NH₃)₆]₃+ (RuHex) in the electrolyte via electrostatic interaction. Upon target ATP binding, the aptamer confined onto electrode surface was disassociated from the cDNA oligonucleotides into the solution. Such surface density change of DNA lead to the decrease of chronocoulometric signal for the RuHex which confined on the electrode surface. The chronocoulometric signals showed a linear relationship with logrithm of ATP concentration in the range of 1 nmol/L to 100 μmol/L, and the detection limit of this aptamer sensor could reach 0.5 nmol/L (S/N=3). This aptamer sensor could be regenerated 5 times by simple steps. With this aptamer sensor, the basal level of ATP in the brain cortex micorodialysate was determined to be 19.2±3.7 nmol/L (n=3).

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

基于计时库仑技术的可再生型三磷酸腺苷适配体电化学传感器的研究

English

A Reusable Chronocoulometric Aptamer Sensor for Detection of Adenosine Triphosphate

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于计时库仑技术的可再生型三磷酸腺苷适配体电化学传感器的研究

English

A Reusable Chronocoulometric Aptamer Sensor for Detection of Adenosine Triphosphate

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