血清脂质组学研究中多重离子化液相色谱-质谱方法的比较

引用本文: 王中华, 陈艳华, 徐婧, 陈蕙莉, 周霞, 肖新华, 平凡, 贺玖明, 再帕尔·阿不力孜, 张瑞萍. 血清脂质组学研究中多重离子化液相色谱-质谱方法的比较[J]. 分析化学, 2017, 45(5): 674-680. doi: 10.11895/j.issn.0253-3820.160863 shu

Citation: WANG Zhong-Hua, CHEN Yan-Hua, XU Jing, CHEN Hui-Li, ZHOU Xia, XIAO Xin-Hua, PING Fan, HE Jiu-Ming, ABLIZ Zeper, ZHANG Rui-Ping. Comparison of Multiple Ionization Methods Coupled with Liquid Chromatography-Mass Spectrometry in Lipidomics Study of Serum Samples[J]. Chinese Journal of Analytical Chemistry, 2017, 45(5): 674-680. doi: 10.11895/j.issn.0253-3820.160863 shu

血清脂质组学研究中多重离子化液相色谱-质谱方法的比较

1.
中国医学科学院/北京协和医学院药物研究所, 天然药物活性物质与功能国家重点实验室, 北京 100050;
2.
中央民族大学生命与环境科学学院, 生物成像与系统生物学研究中心, 北京 100081;
3.
中国医学科学院/北京协和医学院, 北京协和医院, 北京 100730
基金项目:
本文系国家高技术研究发展计划863项目（No. 2014AA021101）资助

摘要: 系统地比较了3种常用的离子化技术电喷雾电离（ESI）、大气压化学电离（APCI）、大气压光致电离（APPI）对脂类化合物的离子化效率、检测灵敏度和覆盖范围，以探讨多重离子化液相色谱-质谱（LC-MS）方法在血清脂质组学研究中的适用性。血清样本经甲基叔丁基醚萃取后，采用Ascentiss Express C₈ 色谱柱（150 mm×2.1 mm，2.7 μm）和二元线性梯度洗脱分离，流动相（A）为乙腈-水（3∶2，V/V，含0.1%甲酸，10 mmol/L甲酸铵），B为异丙醇-乙腈（9∶1，V/V，含0.1%甲酸，10 mmol/L甲酸铵），分别采用ESI、APCI和APPI离子源正、负离子模式进行质谱检测。结果表明，ESI离子源对脂肪酸类、甘油脂类、甘油磷脂类化合物、鞘磷脂类化合物的离子化效率最高，对异戊烯醇脂类化合物的离子化效率与APPI离子源相当，APPI离子源对胆固醇（酯）类化合物的检测灵敏度最高，APCI离子源对各类化合物的检测灵敏度均低于ESI或APPI离子源；采用ESI和APPI离子源相结合的LC-MS脂质组学分析方法可以提高分析方法的整体灵敏度和血清中脂类信息检测的完整性。

关键词:

English

Comparison of Multiple Ionization Methods Coupled with Liquid Chromatography-Mass Spectrometry in Lipidomics Study of Serum Samples

1.
State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China;
2.
Centre for Imaging & Systems Biology, College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, China;
3.
Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China
Received Date: 25 November 2016
Revised Date: 17 February 2017
Fund Project:
This work was supported by the National High Technology Research and Development Program of China (No. 2014AA021101).

Abstract: Three commonly used ionization techniques, including electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI), and atmospheric pressure photoionization (APPI) were compared systemically for their ionization ability, sensitivity and coverage for detecting lipids, aiming to explore their applicability in lipidomics study of serum samples. Serum samples were pretreated by liquid-liquid extraction with methyl tertiary butyl ether and then introduced into a LC-MS system equipped with electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI), and atmospheric pressure photoionization (APPI) source. Chromatographic separation was performed on an Ascentiss Express C8 column (150 mm×2.1 mm, 2.7 μm). A binary gradient system of acetonitrile-water (3∶2, V/V) and isoropyl alcohol-acetonitrile (9∶1, V/V), both containing 10 mmol/L ammonium formate and 0.1% formic acid, was used as eluent A and B, respectively. Data were acquired in both positive and negative ion modes for each ionization technique. The results showed that fatty acids (FFAs), phospholipids (PLs), sphingophosphalipids (SPs), glycerolipids (GLs), could be ionized most efficiently under ESI source, while APPI source gave the best sensitivity for cholesterol (CHOL), cholesterol esters (CEs) and Sphinganine (Sphingolipids, SPs). Prenol lipids could be ionized equally well under ESI and APPI source, and that the application of ESI and APPI integrated approach was expected to enhance the overall sensitivity of the method and provided more comprehensive lipids information for the global serum lipidomics study.

Key words:

Serum lipidomics
/ Electrospray ionization
/ Atmospheric pressure chemical ionization
/ Atmospheric pressure photoionization
/ Liquid chromatography-mass spectrometry

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血清脂质组学研究中多重离子化液相色谱-质谱方法的比较

English

Comparison of Multiple Ionization Methods Coupled with Liquid Chromatography-Mass Spectrometry in Lipidomics Study of Serum Samples

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中国化学会秘书处

血清脂质组学研究中多重离子化液相色谱-质谱方法的比较

English

Comparison of Multiple Ionization Methods Coupled with Liquid Chromatography-Mass Spectrometry in Lipidomics Study of Serum Samples

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

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CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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