基于超声喷泉的超声雾化结合固相萃取法提取西洋参叶中8种人参皂苷

引用本文: 张培旭, 金永日, 崔俐, 吴谦, 黎鹏, 张寒琦, 李绪文. 基于超声喷泉的超声雾化结合固相萃取法提取西洋参叶中8种人参皂苷[J]. 分析化学, 2018, 46(4): 594-600. doi: 10.11895/j.issn.0253-3820.171117 shu

Citation: ZHANG Pei-Xu, JIN Yong-Ri, CUI Li, WU Qian, LI Peng, ZHANG Han-Qi, LI Xu-Wen. Extraction of Eight Ginsenosides from Leaves of Panax quinquefolium L. By Ultrasoinc Fountain-based Ultrasonic-assisted Nebulization Extraction Coupled with Solid Phase Extraction[J]. Chinese Journal of Analytical Chemistry, 2018, 46(4): 594-600. doi: 10.11895/j.issn.0253-3820.171117 shu

基于超声喷泉的超声雾化结合固相萃取法提取西洋参叶中8种人参皂苷

张培旭 ^1,2, ,
金永日 ^1, ,
崔俐 ^2, ,
吴谦 ^1, ,
黎鹏 ^1, ,
张寒琦 ^1, ,
李绪文 ^1,

1.
吉林大学化学学院, 长春 130021;
2.
吉林大学第一医院神经内科, 长春 130021

摘要: 实验使用超声喷泉替代气溶胶进行传质，改进了超声雾化提取-固相萃取法，设计了实验装置，并用于西洋参叶中的8种常见人参皂苷的提取。对提取剂种类、体积、pH值和盐浓度，洗脱剂种类和体积以及吸附剂质量等影响因素进行了考察与优化。在最优条件下，8种人参皂苷的回收率在96.3%~110.6%之间，RSD在2.8%~4.3%之间，本方法对8种人参皂苷均具有良好的提取率。与传统的超声雾化提取-固相萃取法相比，本方法简化了提取装置，缩短了提取时间，提高了提取效率。

关键词:

English

Extraction of Eight Ginsenosides from Leaves of Panax quinquefolium L. By Ultrasoinc Fountain-based Ultrasonic-assisted Nebulization Extraction Coupled with Solid Phase Extraction

1.
College of Chemistry, Jilin University, Changchun 130021, China;
2.
Department of Neurology, First Hospital, Jilin University, Changchun, 130021, China
Received Date: 21 July 2017
Revised Date: 21 January 2018

Abstract: A new ultrasonic-assisted extraction (UANE) method coupled with solid phase extraction (SPE) using ultrasonic fountain was established for the extraction of eight common ginsenosides from leaves of Panax quinquefolium L. The extraction system has been designed and several experimental parameters, including the type and volume of extraction solvent, pH value and salt concentration of extraction solvent,type and volume of elution solvent,and amount of C₁₈, extraction time were examined and optimized. Under the optimal conditions, the recoveries of ginsenosides were in the range of 96.3%-110.6%, the relative standard deviations (RSDs) were in the range of 2.8%-4.3%, indicating that the method has a good performance for the extraction of these ginsenosides. Compared with traditional UANE-SPE method, the modified method simplified the extraction device, shortened the extraction time and improved the extraction efficiency.

Key words:

Ultrasonic-assisted nebulization extraction
/ Solid phase extraction
/ Ultrasonic fountain
/ Panax quinquefolium L.
/ Ginsenosides

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

基于超声喷泉的超声雾化结合固相萃取法提取西洋参叶中8种人参皂苷

English

Extraction of Eight Ginsenosides from Leaves of Panax quinquefolium L. By Ultrasoinc Fountain-based Ultrasonic-assisted Nebulization Extraction Coupled with Solid Phase Extraction

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

基于超声喷泉的超声雾化结合固相萃取法提取西洋参叶中8种人参皂苷

English

Extraction of Eight Ginsenosides from Leaves of Panax quinquefolium L. By Ultrasoinc Fountain-based Ultrasonic-assisted Nebulization Extraction Coupled with Solid Phase Extraction

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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