芹菜根细胞的超微结构与铅形态特征分析

引用本文: 柳检, 罗立强. 芹菜根细胞的超微结构与铅形态特征分析[J]. 分析化学, 2018, 46(9): 1479-1485. doi: 10.11895/j.issn.0253-3820.181253 shu

Citation: LIU Jian, LUO Li-Qiang. Analysis of Ultrastructure of Root Cell and Lead Speciation in Celery[J]. Chinese Journal of Analytical Chemistry, 2018, 46(9): 1479-1485. doi: 10.11895/j.issn.0253-3820.181253 shu

芹菜根细胞的超微结构与铅形态特征分析

柳检 ,
罗立强

国家地质实验测试中心, 北京 100037
基金项目:
本文系国家重点研发计划项目（No.2016YFC0600603）、国家自然科学基金项目（Nos.20775018，41201527）、国家高新技术研究发展计划（863）项目（No.2007AA06Z124）和中国地质调查项目（No.DD20160340）资助

摘要: 植物细胞是植物响应重金属胁迫的重要生化反应场所，探索细胞层级中铅（Pb）的分布与形态转化是揭示有害元素对蔬菜毒性作用机理的重要途径。本研究以芹菜为例，利用能量色散透射电子显微镜（TEM-EDS）和X射线吸收近边结构谱（XANES）技术，考察了Pb胁迫后芹菜根细胞的超微结构、元素分布和形态转化。研究发现，Pb胁迫显著改变了芹菜根细胞的超微结构，导致胞间间隙加大、细胞壁增厚和质膜变糙；芹菜根、茎和叶对Pb具有显著且不同的形态转化功能，可将无机铅（Pb（NO₃）₂）转化为有机铅形态；芹菜根细胞中62.7%的Pb以Pb₅（PO₄）₃Cl形态沉积在根细胞壁，根和茎中有机铅（Pb（Ac）₂·3H₂O）约占34.4%-37.3%，叶中总有机铅（Pb（C₁₇H₃₅COO）₂和Pb（Ac）₂·3H₂O）占63.3%。结果表明，芹菜细胞的Pb-磷酸盐来源于植物体的生物合成，而非外界根际环境，芹菜根际环境的Pb能够以Pb-Ac的络合物形式进入根细胞，一部分转化成Pb₅（PO₄）₃Cl沉积于细胞壁，实现Pb的解毒和耐受，少部分经茎长程运输至叶片以Pb-大分子有机酸/磷酸盐的形式贮存。

关键词:

English

Analysis of Ultrastructure of Root Cell and Lead Speciation in Celery

LIU Jian ,
LUO Li-Qiang

National Research Center for Geoanalysis, Beijing 100037, China
Received Date: 16 April 2018
Revised Date: 27 June 2018
Fund Project:
This work was supported by the National Key Research and Development Program of China (No. 2016YFC0600603),the National Natural Science Foundation of China (Nos. 20775018, 41201527), the National High Technology Research and Development Program of China (No. 2007AA06Z124) and the Pproject of China Geological Survey (No. DD20160340).

Abstract: Plant cells are important biochemical reaction sites for responding to heavy metal stress. Exploring the distribution and speciation of lead (Pb) in celery at the cellular level is an important way to reveal the toxic mechanism of harmful elements on vegetables. In this study, ultrastructural alterations, localization of Pb in root cell and Pb speciation in celery were investigated by energy dispersive transmission electron microscopy (TEM-EDS) and X-ray absorption near edge spectroscopy (XANES). The result showed that Pb stress significantly changed the ultrastructure of celery root cells, increased intercellular spaces, thickened cell walls, and roughened plasma membrane. The roots, stems and leaves of celery had different speciation transformation functions for Pb, which could convert inorganic lead (Pb(NO₃)₂) into organic lead form. About 62.7% of Pb in celery root cells deposited in the cell wall as the form of Pb₅(PO₄)₃Cl, which showed the tolerance and detoxification of plant for Pb. The organic lead (Pb(Ac)₂·3H₂O) in roots and stems were approximately from 34.4 to 37.3%, while total organic lead (Pb(C₁₇H₃₅COO)₂ and Pb(Ac)₂·3H₂O) in leaves accounted for 63.3%. This study indicated that Pb-phosphate in plant cells was derived from the biosynthesis by plants rather than the external rhizosphere environment. Pb in the rhizosphere of celery could enter the root cell as the form of Pb-Ac complex, and a part of Pb was converted into Pb₅(PO₄)₃Cl deposition in the cell wall to detoxify and tolerate of Pb, and a small part of Pb was transported to the leaves and accumulated as the form of macromolecular Pb-organic acid and Pb-phosphate.

Key words:

Vegetable
/ Cell
/ Ultrastructure
/ Lead speciation
/ X-ray absorption near edge spectroscopy

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芹菜根细胞的超微结构与铅形态特征分析

English

Analysis of Ultrastructure of Root Cell and Lead Speciation in Celery

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

芹菜根细胞的超微结构与铅形态特征分析

English

Analysis of Ultrastructure of Root Cell and Lead Speciation in Celery

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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