肾透明细胞癌组织S-棕榈酰化修饰蛋白质组的分析

引用本文: 郭佳, 魏丽萍, 陈志营, 何成彦. 肾透明细胞癌组织S-棕榈酰化修饰蛋白质组的分析[J]. 分析化学, 2021, 49(9): 1563-1571. doi: 10.19756/j.issn.0253-3820.210590 shu

Citation: GUO Jia, WEI Li-Ping, CHEN Zhi-Ying, HE Cheng-Yan. Proteome Analysis of S-Palmitoylation Modification in Clear Cell Renal Cell Carcinoma[J]. Chinese Journal of Analytical Chemistry, 2021, 49(9): 1563-1571. doi: 10.19756/j.issn.0253-3820.210590 shu

肾透明细胞癌组织S-棕榈酰化修饰蛋白质组的分析

郭佳 ^1, ,
魏丽萍 ^2, ,
陈志营 ^{1,
,} ,
何成彦 ^{2,
,}

1.
吉林大学中日联谊医院呼吸科, 长春 130033;
2.
吉林大学中日联谊医院检验科, 长春 130033

通讯作者: 陈志营,E-mail:zychen@jlu.edu.cn; 何成彦,E-mail:chengyanhe469@vip.sina.com

基金项目:
吉林省科技厅项目（Nos.20190201215JC，20200404193YY）资助。

摘要: 肾透明细胞癌中介导蛋白质棕榈酰化修饰的蛋白酰基转移酶表达异常，提示肾透明细胞癌可能存在蛋白质棕榈酰化修饰异常改变，并与肿瘤代谢紊乱或致癌信号通路的激活密切相关。本研究采用优化的酰基-生物素置换法（Acyl-biotinyl exchange，ABE），结合蛋白质组学技术，对肾透明细胞癌组织中S-棕榈酰化修饰蛋白进行全面分析，共鉴定出92种高可信度的棕榈酰化修饰蛋白，包括细胞骨架相关蛋白4、磷酸丙糖异构酶、脂肪分化相关蛋白、膜联蛋白A4、微管相关蛋白tau蛋白和钙联蛋白等。通过蛋白质印迹分析技术对其中的脂肪分化相关蛋白（Adipose differentiation-related protein，ADRP）棕榈酰化修饰进行了验证。本研究提供了一种改进的适合分析组织样本的ABE实验方法，所得肾透明细胞癌组织中的S-棕榈酰化修饰蛋白的分析结果可为肾透明细胞癌早期诊断、治疗和预后的分子靶点等方面的研究提供基础数据，对进一步深入研究肾透明细胞癌发病机制具有重要的参考价值。

关键词:

English

Proteome Analysis of S-Palmitoylation Modification in Clear Cell Renal Cell Carcinoma

GUO Jia ^1, ,
WEI Li-Ping ^2, ,
CHEN Zhi-Ying ^{1,
,} ,
HE Cheng-Yan ^{2,
,}

1.
Department of Respiratory, China-Japan Union Hospital of Jilin University, Changchun 130033, China;
2.
Department of Clinical Laboratory, China-Japan Union Hospital of Jilin University, Changchun 130033, China

Corresponding author: CHEN Zhi-Ying, ; HE Cheng-Yan,

Received Date: 26 June 2021
Revised Date: 05 August 2021
Fund Project:
Supported by the Jilin Province Science and Technology Department, China (Nos.20190201215JC, 20200404193YY).

Abstract: The abnormal expression of protein acyltransferase that is responsible for protein palmitoylation in renal clear cell carcinoma suggests that there may be abnormal changes of S-palmitoylation in renal clear cell carcinoma, which is closely related to metabolic disorder or activation of carcinogenic signaling pathway. In the present study, the optimized acyl-biotinyl exchange (ABE) method combined with proteomic techniques was used to analyze S-palmitoylated proteins in clear cell renal cell carcinoma. A total of 92 palmitoylated proteins were identified with high reliability, and the S-palmitoylation of adipose differentiation related protein (ADRP) was verified by Western blotting. This study provides an improved ABE method suitable for analyzing tissue samples. The identification of palmitoylated proteins in clear cell renal cell carcinoma can provide primary data for the molecular targets of early diagnosis, treatment and prognosis of clear cell renal carcinoma, which are of great significance for further study of the pathogenesis of clear cell renal carcinoma.

Key words:

S-palmitoylation
/ Clear cell renal cell carcinoma
/ Proteome
/ Acyl-biotin exchange
/ Adipose differentiation related proteins

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

肾透明细胞癌组织S-棕榈酰化修饰蛋白质组的分析

通讯作者: 陈志营,E-mail:zychen@jlu.edu.cn; 何成彦,E-mail:chengyanhe469@vip.sina.com

English

Proteome Analysis of S-Palmitoylation Modification in Clear Cell Renal Cell Carcinoma

Corresponding author: CHEN Zhi-Ying, ; HE Cheng-Yan,

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

肾透明细胞癌组织S-棕榈酰化修饰蛋白质组的分析

通讯作者: 陈志营,E-mail:zychen@jlu.edu.cn; 何成彦,E-mail:chengyanhe469@vip.sina.com

English

Proteome Analysis of S-Palmitoylation Modification in Clear Cell Renal Cell Carcinoma

Corresponding author: CHEN Zhi-Ying, ; HE Cheng-Yan,

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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