电喷雾离子迁移谱检测氨基酸及多肽的研究

引用本文: 孔景临, 刘卫卫, 丁俊杰, 张琳, 李宝强, 秦墨林, 陈创, 李海洋. 电喷雾离子迁移谱检测氨基酸及多肽的研究[J]. 分析化学, 2017, 45(11): 1583-1588. doi: 10.11895/j.issn.0253-3820.171052 shu

Citation: KONG Jing-Lin, LIU Wei-Wei, DING Jun-Jie, ZHANG Lin, LI Bao-Qiang, QIN Mo-Lin, CHEN Chuang, LI Hai-Yang. Detection of Amino Acids and Polypeptides by Electrospray Ionization-Ion Mobility Spectrometry[J]. Chinese Journal of Analytical Chemistry, 2017, 45(11): 1583-1588. doi: 10.11895/j.issn.0253-3820.171052 shu

电喷雾离子迁移谱检测氨基酸及多肽的研究

孔景临 ^1, ,
刘卫卫 ^1, ,
丁俊杰 ^1, ,
张琳 ^1, ,
李宝强 ^1, ,
秦墨林 ^1, ,
陈创 ^2, ,
李海洋 ^2,

1.
防化研究院, 北京 102205;
2.
中国科学院大连化学物理研究所, 大连 116023

摘要: 建立了氨基酸及多肽的电喷雾离子迁移谱检测方法。采用自制的电喷雾离子迁移谱装置，在室温条件下以甲醇为溶剂，空气为漂移气体，流速为1000 mL/min，电喷液流速为2μL/min，测试了甘氨酸、胱氨酸、组氨酸、精氨酸4种氨基酸及缓激肽片段（1~7）和P物质2种多肽的离子迁移谱，计算出上述化合物的约化迁移率。离子迁移谱图反映出化合物的结构信息，具有指纹谱特征。此装置在1 min的检测时间内对P物质的检测灵敏度达到855 ng/mL。结果表明，电喷雾离子迁移谱可用于氨基酸及多肽类化合物的现场快速鉴定。

关键词:

English

Detection of Amino Acids and Polypeptides by Electrospray Ionization-Ion Mobility Spectrometry

1.
Institute of Chemical Defense, Beijing 102205, China;
2.
Dalian Institute of Chemical Physics Chinese Academy of Sciences, Dalian 116023, China
Received Date: 30 June 2017
Revised Date: 08 September 2017

Abstract: The electrospray ionization-ion mobility spectrometric (ESI-IMS) technique has the potential as an analytical separation tool in analyzing polypeptides and amino acids for fast screening unknown samples in anti-chemical and biological terror attacks. A method for detecting several polypeptides and amino acids was developed based on ESI-IMS using air as drift gas at room temperature. The ion mobility of four amino acids and two polypeptides dissolved in methanol was determined on the system at elution rate of 2μL/min. The spectra of these compounds had characteristics of finger-printing maps. The limit of detection of this instrument for Substance P could reach 855 ng/mL in 1 min. The results showed that a small, self-contained ESI-IMS instrument with reservoirs of air could be used to quickly detect and accurately identify polypeptides and amino acids.

Key words:

1. [1]
  Ellison H. Handbook of Chemical and Biological Warfare Agents, Translated by Institute of Chemical Defense, Beijing:YiWen Publishing Military, 2009:564-579 Ellison H. 化学与生物战剂手册, 防化研究院译, 第二版, 军事谊文出版社:564-579
2. [2]
  JIANG Dan-Dan, PENG Li-Ying, ZHOU Qing-Hua, CHEN Chuang, LIU Ji-Wei, WANG Shuang, LI Hai-Yang. Chinese J. Anal. Chem., 2016, 44(11):1671-1678 蒋丹丹, 彭丽英, 周庆华, 陈创, 刘骥巍, 王爽, 李海洋. 分析化学, 2016, 44(11):1671-1678
3. [3]
  Sferopoulos R. A Review of Chemical Warfare Agent Detector Technologies and Commercial-Off-The-Shelf Items, Translated by LIANG Chi-Yong, XIA Zhi-Qiang, WANG Man-Lin, LI Tie-Hu. Beijing:YiWen Publishing Military, 2013:25-44 Sferopoulos R. 化学毒剂检测器技术与装备评价. 梁赤勇, 夏志强, 王曼琳, 李铁虎译. 北京:军事谊文出版社, 2013:25-44
4. [4]
  Wittmer D, Chen Y H, Luckenbill W K, Hill H H. Anal. Chem., 1994, 66:2348-2355
5. [5]
  Shvartsburg A A, Creese A J, Smith R D, Cooper H J. Anal. Chem., 2010, 82:8327-8334
6. [6]
  Isenberg S L, Armistead P M, Glish G L. J. Am. Soc. Mass Spectrom., 2014, 25:1592-1599
7. [7]
  Young L M, Saunders J C, Mahood R A, Revill C H, Foster R J, Ashcroft A E, Radford S E. Methods, 2016, 95:62-69
8. [8]
  Shvartsburg A A, Creese A J, Smith R D, Cooper H J. Anal. Chem., 2011, 83:6918-6923
9. [9]
  Jafari M T, Rezaei B, Javaheri M. Food Chem., 2011, 126:1964-1970
10. [10]
  Midey A J, Patel A, Moraff C, Krueger C A, Wu C. Talanta, 2013, 116(22):77-83
11. [11]
  Sheibani A, Tabrizchi M, Ghaziaskar H S. Talanta, 2008, 75:233-238
12. [12]
  Hilton C K, Krueger C A, Midey A J, Osgood M, Wu J. Inter. J. Mass Spectrom., 2010, 298(1):64-71
13. [13]
  LIU Wen-Jie, LI Guo-Zhu, LUO Bi, MENG Qin-Yan, LU Ya-Ling, YIN Du-Ling. Chinese J. Anal. Chem., 2015, 43(5):788-793 刘文杰, 李国柱, 罗碧, 孟庆艳, 卢亚玲, 尹笃林. 分析化学, 2015, 43(5):788-793
14. [14]
  ZHANG Zhi-Gang, FANG En-Hua, HAO Yu-Lei, ZHU Jun. J. Food Safety Quality, 2015, 6(6):1-7 张志刚, 方恩华, 郝玉蕾, 朱军. 食品安全质量检测学报, 2015, 6(6):1-7
15. [15]
  CHEN Chuang, WANG Wei-Guo, LIANG Qian-Qian, ZHOU Qing-Hua, PENG Li-Ying, WEN Meng, JU Bang-Yu, ZHAO Kun, LIU Jun, LI Hai-Yang, Chinese J. Chromatogr., 2013, 31(4):386-391 陈创, 王卫国, 梁茜茜, 周庆华, 彭丽英, 温萌, 鞠帮玉, 赵琨, 刘军, 李海洋. 色谱, 2013, 31(4):386-391
16. [16]
  LIN Fu-Sheng, ZHONG Ming-Nai, PEI Cheng-Xin, LUO Qing-Cong, ZHANG Shou-Lan, CHEN Chang-Ying, DU Xiu-Bao, LEI Guang-Qing, FAN Chong-Xu. J. Health Toxicol., 2001, 15(3):5-8 林福生, 钟明鼐, 裴承新, 罗庆聪, 张守兰, 陈常英, 杜秀宝, 雷光清, 范崇旭. 卫生毒理学杂志, 2001, 15(3):5-8
17. [17]
  Eiceman G A, Karpas Z. Ion Mobility Spectrometry. Translated by GUO Cheng-Hai, CAO Shu-Ya, Beijing:National Defense Industry Press, 2010:238-250 Eiceman G A, Karpas Z. 离子迁移谱. 郭成海, 曹树亚译. 北京:国防工业出版社, 2010:238-250

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沈阳化工大学材料科学与工程学院沈阳 110142

电喷雾离子迁移谱检测氨基酸及多肽的研究

English

Detection of Amino Acids and Polypeptides by Electrospray Ionization-Ion Mobility Spectrometry

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

电喷雾离子迁移谱检测氨基酸及多肽的研究

English

Detection of Amino Acids and Polypeptides by Electrospray Ionization-Ion Mobility Spectrometry

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

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