质子转移反应-飞行时间质谱法鉴定6种家畜原乳气味物质及特征分析

引用本文: 吴艳, 郭军, 王越男, 韦航, 邵冬蕊. 质子转移反应-飞行时间质谱法鉴定6种家畜原乳气味物质及特征分析[J]. 分析化学, 2022, 50(4): 643-658. doi: 10.19756/j.issn.0253-3820.221003 shu

Citation: WU Yan, GUO Jun, WANG Yue-nan, WEI Hang, SHAO Dong-rui. Identification and Analysis on Flavor Compounds of Raw Milk of Six Kinds of Livestocks by Proton Transfer Reaction-Time of Flight-Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, 2022, 50(4): 643-658. doi: 10.19756/j.issn.0253-3820.221003 shu

质子转移反应-飞行时间质谱法鉴定6种家畜原乳气味物质及特征分析

吴艳 ^1, ,
郭军 ^{1,
,} ,
王越男 ^1, ,
韦航 ^2, ,
邵冬蕊 ^1,

1.
内蒙古农业大学食品科学与工程学院, 呼和浩特 010018;2 福建省农业科学院农业质量标准与检测技术研究所, 福建省农产品质量安全重点实验室, 福州 350003

通讯作者: 郭军,E-mail:guojunge@imau.edu.cn

基金项目:
国家自然科学基金项目(No.31760489)资助

摘要: 家畜乳气味物质主要包括酸类、酯类、酮类、醛类和烷烃类等,但原乳气味物质的具体构成可能与物种、饲草料及代谢异常等复杂因素有关。本研究采集蒙古马、双峰驼、荷斯坦牛、萨能山羊、麦洼牦牛和摩拉水牛6种原乳样品共53份,用质子转移反应-飞行时间质谱检测鉴定乳的气味物质,进行多变量统计分析观察6种原乳以其气味物质自然聚类的特征。构建SIMCA和PCA-Class判别模型,评价气味鉴定家畜原乳物种、地区(地方品种)真实性的可行性。在蒙古马、双峰驼、荷斯坦牛、萨能山羊、麦洼牦牛和摩拉水牛乳中分别鉴定出27、23、16、21、21和18种气味物质,主要为酮类、酸类、醛类、醇类和含硫化合物等。多变量统计分析结果表明,6种家畜原乳以物种聚类,并且距离符合物种分类学规律,蒙古马乳和双峰驼乳均以产地分开聚类,同产地阿拉善驼和新疆驼乳聚类分离,但不影响地区聚类。对6种家畜原乳物种来源的判别,SIMCA和PCA-Class模型外部验证正确率分别为92.72%和98.38%;对不同地区和品种的蒙古马乳和双峰驼乳外部验证准确率大于83.00%。本研究结果表明,原乳气味质谱指纹建模可用于判别物种、地区/地方品种,为家畜乳物种、地区/地方品种的真实性判别提供新的策略和方法。

关键词:

English

Identification and Analysis on Flavor Compounds of Raw Milk of Six Kinds of Livestocks by Proton Transfer Reaction-Time of Flight-Mass Spectrometry

WU Yan ^1, ,
GUO Jun ^{1,
,} ,
WANG Yue-nan ^1, ,
WEI Hang ^2, ,
SHAO Dong-rui ^1,

1.
College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China;2 Fujian Key Laboratory of Agro-products Quality & Safety, Institute of Agricultural Quality Standards and Testing Technology Research Fujian Academy of Agricultural Sciences, Fuzhou 350003, China

Corresponding author: GUO Jun, guojunge@imau.edu.cn

Received Date: 03 January 2022
Revised Date: 22 March 2022
Fund Project:
Supported by the National Natural Science Foundation of China(No. 31760489)

Abstract: Flavor compounds of livestock milk mainly include acids, esters, ketones, aldehydes and alkanes, however the composition of flavor compound of each animal milk may related to species, forage, metabolism disorder, as well other complicated factors. In this study, a total of 53 raw milk samples of Mongolia horse, Bactrian camel, Holstein cow, Saanen goat, Maiwa yak and Murrah buffalo were collected. Flavor compounds of raw milk were determined and identified by proton transfer reaction-time of flight-mass spectrometry(PTR-TOF-MS), and multivariate statistical analysis was conducted to observe the natural clustering characteristics of six kinds of livestock milk by their flavor compounds. SIMCA and PCA-Class discrimination model were constructed to evaluate the feasibility of authentication on species, geographical origin/local varieties of raw milk by their flavor compounds. As results, there were 27, 23, 16, 21, 21 and 18 kinds of flavor compounds identified in the raw milk of Mongolia horse, Bactrian camel, Holstein cow, Saanen goat, Maiwa yak and Murrah buffalo, respectively, mainly were ketones, acids, aldehydes, alcohols and sulfur compounds. Multivariate statistical analysis showed that six kinds of livestock raw milk were clustered into six groups and the clustering distance consistent with taxonomy of these species. Mongolia horse and Bactrian camel milk clusters were both separated by geographical origins. Alxa camels and Xinjiang camel milk clusters from same origin were separated but did not influence original separation. Six species of raw milk of livestock were identified, and the accuracy of external verification of SIMCA and PCA-class model were 92.72% and 98.38% respectively. The external verification accuracy of Mongolian horse milk and Bactrian camel milk from different geographical regions and local varieties were above 83.00%. The results showed that the authentication of species, geographical origin/local varieties of animal milk by flavor compounds or MS fingerprint modeling was promising. The study introduced another innovative strategy and methodology to authentication of species, geographical origin/local varieties of animal milk.

Key words:

Milk
/ Proton transfer reaction-time of flight-mass spectrometry
/ Flavor compounds
/ Principal component analysis
/ Partial least squares-discrimination analysis
/ Soft independent modeling of class analogies

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沈阳化工大学材料科学与工程学院沈阳 110142

质子转移反应-飞行时间质谱法鉴定6种家畜原乳气味物质及特征分析

通讯作者: 郭军,E-mail:guojunge@imau.edu.cn

English

Identification and Analysis on Flavor Compounds of Raw Milk of Six Kinds of Livestocks by Proton Transfer Reaction-Time of Flight-Mass Spectrometry

Corresponding author: GUO Jun, guojunge@imau.edu.cn

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

质子转移反应-飞行时间质谱法鉴定6种家畜原乳气味物质及特征分析

通讯作者: 郭军,E-mail:guojunge@imau.edu.cn

English

Identification and Analysis on Flavor Compounds of Raw Milk of Six Kinds of Livestocks by Proton Transfer Reaction-Time of Flight-Mass Spectrometry

Corresponding author: GUO Jun, guojunge@imau.edu.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

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