纳米基因扩增技术及其应用

引用本文: 桑付明, 李鑫, 刘佳. 纳米基因扩增技术及其应用[J]. 分析化学, 2017, 45(11): 1745-1753. doi: 10.11895/j.issn.0253-3820.170097 shu

Citation: SANG Fu-Ming, LI Xin, LIU Jia. Development of Nano-Polymerase Chain Reaction and Its Application[J]. Chinese Journal of Analytical Chemistry, 2017, 45(11): 1745-1753. doi: 10.11895/j.issn.0253-3820.170097 shu

纳米基因扩增技术及其应用

哈尔滨工业大学(威海)海洋科学与技术学院, 威海 264209
基金项目:
本文系国家自然科学基金（No.21407035）、山东省自然科学基金（No.ZR2014BM021）和威海市大学共建项目（No.2014XGJ15）资助

摘要: 聚合酶链式反应（PCR）是20世纪80年代中期发展起来的一种应用广泛的体外DNA扩增技术，但目前该技术仍然存在着一些问题，如特异性差、灵敏度低和假阳性等。近年来，随着纳米科技的发展，一些纳米粒子如金属纳米粒子、碳纳米材料、量子点和纳米金属氧化物等被引入到PCR反应体系中，即纳米基因扩增技术（NanoPCR）。该技术大幅度提高了PCR的扩增效率、选择性、灵敏度和特异性，推动了生物学技术的发展，具有非常重要的理论意义和应用价值。本文综述了近年来纳米基因扩增技术的主要研究进展、反应机理，并探讨了其应用研究。

关键词:

English

Development of Nano-Polymerase Chain Reaction and Its Application

School of Marine Science and Technology, Harbin Institute of Technology(Weihai), Weihai 264209, China
Received Date: 17 February 2017
Revised Date: 04 September 2017
Fund Project:
This works was supported by the National Natural Science Foundation of China (No. 21407035)

Abstract: Polymerase chain reaction (PCR) has become one of the powerful technique since its invention in 1980s. Nevertheless, PCR technique is still frequently impaired by its low specificity, poor sensitivity, false positive results, etc. Recently, nanomaterials including metal nanoparticles, carbon nanomaterials, quantum dots and nano metal oxide have been added into PCR solution to improve both quality and productivity of PCR. The nanoparticles assisted PCR (NanoPCR) has received considerable attentions due to its unprecedented sensitivity, selectivity and efficiency. In this view, the mainly used nanoparticles in NanoPCR, including gold nanoparticles, quantum dots, carbon nanomaterials, graphene and metallic oxide, was firstly summarized. And then, the possible mechanisms for highly improved sensitivity and selectivity were discussed. Finally, recent applications of NanoPCR were described.

Key words:

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沈阳化工大学材料科学与工程学院沈阳 110142

纳米基因扩增技术及其应用

English

Development of Nano-Polymerase Chain Reaction and Its Application

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

纳米基因扩增技术及其应用

English

Development of Nano-Polymerase Chain Reaction and Its Application

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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