引用本文:
Cheng Cheng Liu, Yun Liu, Hui Xiang Wang, Yan Bo Qi, Peng Yuan Yang, Bao Hong Liu. Apolipoprotein B100 analysis in microchip with electrochemical detection[J]. Chinese Chemical Letters,
2011, 22(11): 1343-1346.
doi:
10.1016/j.cclet.2011.05.028
Citation: Cheng Cheng Liu, Yun Liu, Hui Xiang Wang, Yan Bo Qi, Peng Yuan Yang, Bao Hong Liu. Apolipoprotein B100 analysis in microchip with electrochemical detection[J]. Chinese Chemical Letters, 2011, 22(11): 1343-1346. doi: 10.1016/j.cclet.2011.05.028
Citation: Cheng Cheng Liu, Yun Liu, Hui Xiang Wang, Yan Bo Qi, Peng Yuan Yang, Bao Hong Liu. Apolipoprotein B100 analysis in microchip with electrochemical detection[J]. Chinese Chemical Letters, 2011, 22(11): 1343-1346. doi: 10.1016/j.cclet.2011.05.028
Apolipoprotein B100 analysis in microchip with electrochemical detection
摘要:
Apolipoprotein B100 (apoB-100) is a major protein of the cholesterol-rich low-density lipoprotein (LDL) and reflects a better assessment of total atherogenic burden to the vascular system than LDL. In this work, a simple and sensitive method has been developed to determine picoliter apoB-100s using the PMMA microfluidic chip coupled with electrochemical detection system. This method performs very well with a detectable linear range of 1-800 pg/mL and a detection limit of 1 pg/mL. A real serum sample has further been detected by this microchip-based biosensor. The results show that this kind of method is practicable and has the potential application in clinical analysis and diagnosis.
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关键词:
- Microchip
- / ApoB-100
- / Immunoassay
- / Electrochemical detection
English
Apolipoprotein B100 analysis in microchip with electrochemical detection
Abstract:
Apolipoprotein B100 (apoB-100) is a major protein of the cholesterol-rich low-density lipoprotein (LDL) and reflects a better assessment of total atherogenic burden to the vascular system than LDL. In this work, a simple and sensitive method has been developed to determine picoliter apoB-100s using the PMMA microfluidic chip coupled with electrochemical detection system. This method performs very well with a detectable linear range of 1-800 pg/mL and a detection limit of 1 pg/mL. A real serum sample has further been detected by this microchip-based biosensor. The results show that this kind of method is practicable and has the potential application in clinical analysis and diagnosis.
-
Key words:
- Microchip
- / ApoB-100
- / Immunoassay
- / Electrochemical detection
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