引用本文:
Wen Yu ZHU, Wen Bao CHANG, Yun Xiang CI. Liposome Immunoassay for Determination of Phenytoin[J]. Chinese Chemical Letters,
1998, 9(11): 1035-1038.
Citation: Wen Yu ZHU, Wen Bao CHANG, Yun Xiang CI. Liposome Immunoassay for Determination of Phenytoin[J]. Chinese Chemical Letters, 1998, 9(11): 1035-1038.
Citation: Wen Yu ZHU, Wen Bao CHANG, Yun Xiang CI. Liposome Immunoassay for Determination of Phenytoin[J]. Chinese Chemical Letters, 1998, 9(11): 1035-1038.
Liposome Immunoassay for Determination of Phenytoin
摘要:
Homogeneous liposome immunoassay for the determination of phenytoin was studied. Liposomes were prepared from cholesterol (CH) and phospholipids including dipalmitoyl phosphatidyl ethanolamine (DPPE) for conjugation with thiol-containing antibodies. Hemin chloride was entrapped in the liposome and antibody was modified by reaction with 3-(2-pyridyl-dithio) propionyl N-hydroxysuccinimidc ester (SPDP) to introduce thiol groups for efficient coupling. Antibody-coupled liposomes (immunoliposomes) were incubated with phenytoin and complement, and then with hemin substrate. The amount of hemin released from immunoliposomes, which increases with concentration increase of phenytoin, can be detected rapidly by determining the fluorescence with its substrate p-hydroxyphenyl propionic acid (HPPA) and hydrogen peroxide.
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关键词:
- Iiposome
- / phenytoin
- / liposome immunoassay
English
Liposome Immunoassay for Determination of Phenytoin
Abstract:
Homogeneous liposome immunoassay for the determination of phenytoin was studied. Liposomes were prepared from cholesterol (CH) and phospholipids including dipalmitoyl phosphatidyl ethanolamine (DPPE) for conjugation with thiol-containing antibodies. Hemin chloride was entrapped in the liposome and antibody was modified by reaction with 3-(2-pyridyl-dithio) propionyl N-hydroxysuccinimidc ester (SPDP) to introduce thiol groups for efficient coupling. Antibody-coupled liposomes (immunoliposomes) were incubated with phenytoin and complement, and then with hemin substrate. The amount of hemin released from immunoliposomes, which increases with concentration increase of phenytoin, can be detected rapidly by determining the fluorescence with its substrate p-hydroxyphenyl propionic acid (HPPA) and hydrogen peroxide.
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Key words:
- Iiposome
- / phenytoin
- / liposome immunoassay
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