新疆紫草的毛细管电泳指纹图谱研究

引用本文: 张蕾, 张爱芹, 王嫚, 申刚义. 新疆紫草的毛细管电泳指纹图谱研究[J]. 分析化学, 2017, 45(11): 1727-1733. doi: 10.11895/j.issn.0253-3820.171120 shu

Citation: ZHANG Lei, ZHANG Ai-Qin, WANG Man, SHEN Gang-Yi. Capillary Electrophoresis Fingerprint of Arnebia Euchroma (Royle) Johnst[J]. Chinese Journal of Analytical Chemistry, 2017, 45(11): 1727-1733. doi: 10.11895/j.issn.0253-3820.171120 shu

新疆紫草的毛细管电泳指纹图谱研究

张蕾 ^1, ,
张爱芹 ^2, ,
王嫚 ^1, ,
申刚义 ^1,

1.
中央民族大学中国少数民族传统医学研究院, 北京 100081;
2.
中央民族大学生命与环境科学学院北京市食品环境与健康工程技术研究中心, 北京 100081
基金项目:
本文系国家自然科学基金项目（Nos.81573834、81001595）、国家科技支撑计划项目（No.2014BAC15B04）和中央民族大学一流大学一流学科项目（No.YLDX01013）资助

摘要: 建立了基于毛细管电泳技术的新疆紫草指纹图谱的质量控制方法。优化后的电泳条件：分离柱为50μm×40 cm未涂层毛细管柱，运行缓冲液为pH 8.0、100 mmol/L硼酸盐缓冲液（含25 mmol/L SDS及20%（V/V）无水乙醇），进样量0.5 psi×5 s，分离电压25 kV，检测波长214 nm。应用此条件，35 min内可实现新疆紫草有效成分的高效分离。在方法学验证的基础上，建立了新疆紫草指纹图谱。以新疆紫草对照药材指纹图谱为对照图谱，通过特征指纹峰、S_F'相似度评价、聚类分析对不同购买地的新疆紫草进行质量评价和鉴别。此研究结果与其它中药鉴定方法对照结果一致。本方法准确、可靠、用时短，且具有良好的重现性，为新疆紫草的质量控制与评价提供了一种新的快速有效的鉴别方法。

关键词:

English

Capillary Electrophoresis Fingerprint of Arnebia Euchroma (Royle) Johnst

1.
Institute of Chinese Minority Traditional Medicine, The Key Laboratory of Chinese Minority Traditional Medicine(Ministry of Education of China), Minzu University of China, Beijing, 100081;
2.
College of Life and Environmental Science, Beijing Engineering Resaerch Center of Food Environment and Public Health Minzu University of China, Beijing, 100081
Received Date: 22 July 2017
Revised Date: 29 August 2017
Fund Project:
This work was supported by the National Natural Science Foundation of China (Nos. 81573834, 81001595), the National Science and Technology Support Program, China (No. 2014BAC15B04) and Minzu University of China of the World First-class University and the First-class Discipline (No. YLDX01013).

Abstract: A method of capillary electrophoresis fingerprint was developed for evaluation of the quality of Arnebia euchroma (Royle) Johnst. The samples were separated on a 50 μm×40 cm uncoated capillary separation column at separation voltage of 25 kV with 100 mmol/L borate buffer (pH 8.0) containing 25 mmol/L SDS and 20% (V/V) dehydrated alcohol as running buffer. The injection volume of sample was 0.5 psi×5 s and the detection wavelength was 214 nm. The results indicated that the samples of Arnebia euchroma (Royle) Johnst were well separated and detected in 35 min. With Shikonin peak as reference peak, 6 characteristic peaks of standard Arnebia euchroma (Royle) Johnst were determined. The quality discriminant analyses were accomplished for different kinds of samples named Arnebia euchroma (Royle) Johnst that purchased from eight sources by means of characteristic fingerprint peak analysis, similarity evaluation and cluster analysis. This method had good reproducibility, and could be used for the quality control of Arnebia euchroma (Royle) Johnst.

Key words:

1. [1]
  Chinese Pharmacopoeia Commission. Pharmacopoeia of The People's Republic of China, Part 1. Beijing:The Medicine Science and Technology Press of China, 2015:340 国家药典委员会. 中华人民共和国药典, 一部. 北京:中国医药科技出版社, 2015:340
2. [2]
  ZHAN Zhi-Lai, HU Jun, LIU Tan, KANG Li-Ping, NAN Tie-Gui, GUO Lan-Ping.China Journal of Chinese Materia Medica, 2015, 40(21):4127-4135 詹志来, 胡峻, 刘谈, 康利平, 南铁贵, 郭兰萍. 中国中药杂志, 2015, 40(21):4127-4135
3. [3]
  ZHU Meng-Yuan, WANG Ru-Bing, ZHOU Wen, LI Shao-Shun.Acta Pharmaceutica Sinica, 2012, 47(5):588-593 朱梦媛, 王汝冰, 周文, 李绍顺. 药学学报, 2012, 47(5):588-593
4. [4]
  LI Qiang, DU Si-Miao, ZHANG Zhong-Liang, LYU Chun-Ming, ZHOU Yong-Quan, ZHAO Yan, ZHANG Ning. Chinese Traditional and Herbal Drugs, 2013, 44(22):3095-3104 李强, 杜思邈, 张忠亮, 吕春明, 周永全, 赵燕, 张宁. 中草药, 2013, 44(22):3095-3104
5. [5]
  HUA Yong-Li, WEI Yan-Ming, GUO Yan-Sheng, WU Hai-Yan, QU Ya-Ling.Chinese J. Anal. Chem., 2012, 40(4):602-607 华永丽, 魏彦明, 郭延生, 吴海燕, 曲亚玲. 分析化学, 2012, 40(4):602-607
6. [6]
  ZHAO Chun-Fang, LI Qing-Jie, WANG Lian-Ping, HE Zhong-Mei, LIU Yong-Qiang.Chinese J. Anal. Chem., 2013, 41(1):133-136 赵春芳, 李庆杰, 王莲萍, 何忠梅, 刘永强. 分析化学, 2013, 41(1):133-136
7. [7]
  LIU Jie, DING Wen-Jie, HE Bi-Ying, LUO Lan, HUANG Qiang.Chinese J. Anal. Chem., 2013, 41(4):500-508 柳洁, 丁文婕, 何碧英, 罗兰, 黄蔷. 分析化学, 2013, 41(4):500-508
8. [8]
  William P C B, Raquel O R, Juliana J M P. Asian Pac. J. Trop Biomed., 2017, 7(2):139-143
9. [9]
  JIANG Lin, LI Xiao-Jin, JIA Xiao-Guang. Chinese Journal of Traditional Patent Medicine, 2005, 27(11):1241-1243 姜林, 李晓谨, 贾晓光. 中成药, 2005, 27(11):1241-1243
10. [10]
  CAI Ying, LU Yu, GONG Lin, QIU Rong-Li, HUANG Chong-Fa. Chinese Journal of Traditional Medical Science and Technology, 2016, 23(2):172-174 蔡鹰, 陆瑜, 龚琳, 邱蓉丽, 黄重发. 中国中医药科技, 2016, 23(2):172-174
11. [11]
  LI Fang-Yue, CAO Xue-Lin, HAO Sheng-Yuan, LIU Hui, XING Shao-Rong, XU Hui. Journal of Yantai University (Natural Science and Engineering Edition), 2016, 29(3):187-192 李方悦, 曹雪霖, 郝盛源, 刘卉, 邢绍蓉, 许卉. 烟台大学学报(自然科学与工程版), 2016, 29(3):187-191
12. [12]
  Jing J F, Mu X Y, Qiao J, Su Y, Qi L. Talanta, 2017, 175:451-456
13. [13]
  CHEN Yi. Capillary Electrophoresis and Applications. Beijing:Chemical Industry Press, 2006:6 陈义. 毛细管电泳技术及应用, 第二版. 北京:化学工业出版社, 2006:6
14. [14]
  Mu X Y, Qi L, Qiao J, Yang X Z, Ma H M. Anal. Chim. Acta, 2014, 846:68-74
15. [15]
  Wang Y, Sun G X, Liu Z B, Liu Y C, Gao Y N, Zhang J Q, Ji Z C, Chen X X.J. Sep. Sci., 2014, 37:3571-3578
16. [16]
  XU Jing, WANG Cheng-Fang, DU Shu-Shan, MENG Fan-Yun, LI Feng.Chinese Traditional Patent Medicine, 2014, 36(3):563-566 许靖, 王成芳, 杜树山, 孟繁蕴, 李峰. 中成药, 2014, 36(3):563-566
17. [17]
  SHEN Gang-Yi, YU Wan-Ting, ZHANG Ai-Qin, LI Shu-Wen, WANG Ning. Chinese Journal of Analysis Laboratory, 2014, 33(5):513-515 申刚义, 于婉婷, 张爱芹, 李书文, 王宁. 分析试验室, 2014, 33(5):513-515
18. [18]
  ZHANG Lei, SHEN Gang-Yi. The 30th Annual Academic Meeting of China Chemical Society, 2016, Dalian, China. 张蕾, 申刚义. 中国化学会第30届学术年会, 2016, 大连.
19. [19]
  LI Quan-Wen, CHEN Zuan-Guang, ZHOU Xie, PAN Ai-Hua, WANG Li-Shi.Chinese J. Anal. Chem., 2006, 34(7):991-994 李全文, 陈缵光, 周勰, 潘爱华, 王立世. 分析化学, 2006, 34(7):991-994
20. [20]
  CHEN Li-Jing, SUN Xiu-Mei, ZHANG Zhao-Wang, HUANG Yan-Liang, ZHOU Ying. Chinese Traditional and Herbal Drugs, 2012, 43(3):487-49 陈丽静, 孙秀梅, 张兆旺, 黄延亮, 周莹. 中草药, 2012, 43(3):487-491
21. [21]
  SUN Guo-Xiang, HOU Zhi-Fei, ZHANG Chun-Ling, BI Kai-Shun, SUN Yu-Qing.Acta Pharmaceutica Sinica, 2007, 42(1):75-80 孙国祥, 侯志飞, 张春玲, 毕开顺, 孙毓庆. 药学学报, 2007, 42(1):75-80
22. [22]
  YANG Yan-Tao, WU Chun-Ying, LIU Wen-Long, SHI Ji-Lian, ZHOU Jin, HE Fu-Yuan. ChinaJournal of Traditional Chinese Medicine and Pharmacy. 2013, 25(8):1431-1435 杨岩涛, 吴春英, 刘文龙, 石继连, 周晋, 贺福元. 中华中医药杂志, 2013, 25(8):1431-1435
23. [23]
  MA Xin, SUN Yu-Qing.Chinese Traditional and Herbal Drugs, 2004, 35(8):876-878 马欣, 孙毓庆. 中草药, 2004, 35(8):876-878
24. [24]
  Dong R F, Su J, Nian H, Shen H, Zhai X, Xin H L, Qin L P, Han T. J. Funct. Foods, 2017, 37:513-522

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沈阳化工大学材料科学与工程学院沈阳 110142

新疆紫草的毛细管电泳指纹图谱研究

English

Capillary Electrophoresis Fingerprint of Arnebia Euchroma (Royle) Johnst

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

新疆紫草的毛细管电泳指纹图谱研究

English

Capillary Electrophoresis Fingerprint of Arnebia Euchroma (Royle) Johnst

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

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CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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