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基于特异性蛋白Gαa的比浊法检测β-葡聚糖

田振华 ,  张昊 ,  于源华

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引用本文: 田振华, 张昊, 于源华. 基于特异性蛋白Gαa的比浊法检测β-葡聚糖[J]. 应用化学, 2016, 33(6): 727-732. doi: 10.11944/j.issn.1000-0518.2016.06.150325 shu
Citation:  TIAN Zhenhua, ZHANG Hao, YU Yuanhua. Turbidimetry Assay for Detection of beta-Glucan by Gαa Binding Protein[J]. Chinese Journal of Applied Chemistry, 2016, 33(6): 727-732. doi: 10.11944/j.issn.1000-0518.2016.06.150325 shu

基于特异性蛋白Gαa的比浊法检测β-葡聚糖

  • 1.

    长春理工大学生命科学与技术学院 长春 130022

    • 通讯作者: 于源华,教授;Tel:0431-85583404;E-mail:yuyuanhua8888@126.com;研究方向:生物分析检测
  • 基金项目:

    吉林省科技厅资助项目（20140309013YY）资助 

摘要: 通过基因重组技术，克隆表达了β-葡聚糖特异性结合美洲鲎G因子α亚基片段a（Gαa）蛋白，并建立了β-葡聚糖比浊检测方法。克隆的Gαa基因相对分子质量为40000（1251 bp），浓度为2 g/L，纯度达到98%以上。该蛋白能与βG特异性结合，紫外分光光度计在340 nm下检测的吸光度与βG含量呈正线性相关，线性范围3.125～200 mg/L，R2=0.997，检测限3.125 mg/L。结合力实验表明，该蛋白与βG具有良好的亲和性及特异性。该方法具有成本低、快速、专一性强等特点。

English

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  • 收稿日期:  2015-09-06
  • 网络出版日期:  2015-12-11
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