用于富集磷酸化合物的温度敏感亲和色谱材料的制备及其应用

引用本文: 戴国鑫, 王迪, 王彦, 曹之涵, 戴荣继. 用于富集磷酸化合物的温度敏感亲和色谱材料的制备及其应用[J]. 色谱, 2019, 37(4): 376-382. doi: 10.3724/SP.J.1123.2018.08041 shu

Citation: DAI Guoxin, WANG Di, WANG Yan, CAO Zhihan, DAI Rongji. Preparation and application of temperature-sensitive affinity chromatographic materials for enrichment of phosphoric acid compounds[J]. Chinese Journal of Chromatography, 2019, 37(4): 376-382. doi: 10.3724/SP.J.1123.2018.08041 shu

用于富集磷酸化合物的温度敏感亲和色谱材料的制备及其应用

北京理工大学生命学院, 北京 100081
基金项目:
抗辐射加固技术创新中心创新基金（KFZC2018040208）.

摘要: 基于可逆加成断裂链转移聚合（RAFT）法，通过快速引入官能团的方式合成了固定化金属离子温度敏感亲和色谱材料二氧化硅@聚（N-异丙基丙烯酰胺-co-氨甲基膦酸）-钛离子（Ⅳ）（silica@p（NIPAAm-co-AMPA）-Ti⁴⁺），并应用于HPLC中。采用红外光谱、X-射线光电子能谱、热失重和差示量热法对制备的材料进行表征，证明该材料被成功合成并具有较好的温度敏感性能。将该材料用于三磷酸腺苷（ATP）标准品的富集与分离，实现了仅通过改变固定相温度的方式来达到富集与分离小分子磷酸化合物的目的，为富集分离磷酸化肽段奠定了基础。同时该材料还能以在线补充钛离子的方式延长使用寿命。

关键词:

English

Preparation and application of temperature-sensitive affinity chromatographic materials for enrichment of phosphoric acid compounds

School of Life Science, Beijing Institute of Technology, Beijing 100081, China
Received Date: 27 August 2018
Fund Project:
Foundation of Innovation Center of Radiation Application (No. KFZC2018040208).

Abstract: Based on the surface initiated-reversible addition fragmentation chain transfer (SI-RAFT) method, an immobilized metal ion affinity chromatographic material, silica@ploy(N-isopropylacrylamide-co-(aminomethyl)phosphonic acid)-titanium(Ⅳ) (silica@p(NIPAAm-co-AMPA)-Ti⁴⁺), was synthesized by the method of rapid introduction of functional groups. The synthesis of the material was confirmed by FT-IR and X-ray photoelectron spectroscopy. The grafting ratio and lower critical solution temperature (LCST) of the material were measured by thermal gravimetric analysis (TGA) and differential scanning calorimetry (DSC). The results showed that the material had excellent temperature-sensitive properties and could successfully capture and release adenosine triphosphate successfully. The service life of the material could be extended by online supplementation of Ti⁴⁺.

Key words:

reversible addition fragmentation chain transfer (RAFT)
/ enrichment and separation
/ thermo-responsive chromatographic materials

1. [1] Manning G, Whyte D B, Martinez R, et al. Science, 2002, 298(5600):1912
2. [2] Venter J C, Smith H O, Adams M D. Clin Chem, 2015, 61(9):1207
3. [3] Zhang H, Zha X M, Tan Y, et al. J Biol Chem, 2002, 277(42):39379
4. [4] Cohen P. Nat Cell Biol, 2002, 4(5):127
5. [5] Olsen J V, Blagoev B, Gnad F, et al. Cell, 2006, 127(3):635
6. [6] Golden R J, Chen B B, Li T, et al. Nature, 2017, 542(7640):197
7. [7] Zhang S Y, Chang L F, Alfieri C, et al. Nature, 2016, 533(7602):260
8. [8] Zhao J S, Kim K A, Abo A. Trends Biotechnol, 2009, 27(3) 131
9. [9] Pawson T. Cell, 2004, 116(2):191
10. [10] Blume-Jensen P, Hunter T. Nature, 2001, 411(6835):355
11. [11] Hunter T. Cell, 2000, 100(1):113
12. [12] Harsha H C, Pandey A. Mol Oncol, 2010, 4(6):482
13. [13] Iqbal K, Liu F, Gong C X, et al. Curr Alzheimer Res, 2010, 7(8):656
14. [14] Kotlo K, Johnson K R, Grillon J M, et al. J Proteomics, 2012, 77(24):1
15. [15] Thingholm T E, Jorgensen T J D, Jensen O N, et al. Nat Protoc, 2006, 1(4):1929
16. [16] Lin W, Wang J L, Ying W T, et al. Chinese Journal of Chromatography, 2012, 30(8):763 林威, 王京兰, 应万涛, 等. 色谱, 2012, 30(8):763
17. [17] Shen H L, Alimu K. Chinese Journal of Chromatography, 2018, 36(4):334 申惠莲, 卡哈尔·阿里木. 色谱, 2018, 36(4):334
18. [18] Zhou H J, Palma S D, Preisinger C, et al. J Proteome Res, 2013, 12(1):260
19. [19] Nagase K, Okano T. J Mater Chem B, 2016, 4(39):6381
20. [20] Nagase K, Yamato M, Kanazawa H, et al. Biomaterials, 2018, 153:27
21. [21] Lai J T, Filla D, Shea R. Macromolecules, 2002, 35(18):6754

扫一扫看文章

计量

PDF下载量: 2
文章访问数: 453
HTML全文浏览量: 28

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

用于富集磷酸化合物的温度敏感亲和色谱材料的制备及其应用

English

Preparation and application of temperature-sensitive affinity chromatographic materials for enrichment of phosphoric acid compounds

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

用于富集磷酸化合物的温度敏感亲和色谱材料的制备及其应用

English

Preparation and application of temperature-sensitive affinity chromatographic materials for enrichment of phosphoric acid compounds

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

计量

文章相关

通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

CCS Chemistry：颜徐州、鲍哲南：你的皮肤可能是一片SPMs