引用本文:
刘倩, 钱静, 王坤, 李夜平, 吴向阳. 电化学研究聚乙二醇单甲醚接枝壳聚糖的pH敏感性及对细胞色素c的控制释放[J]. 应用化学,
2012, 29(3): 340-345.
doi:
10.3724/SP.J.1095.2012.00197
Citation: LIU Qian, QIAN Jing, WANG Kun, LI Yeping, WU Xiangyang. pH Sensitivity of Monomethoxypoly(ethylene glycol)grafted-chitosan and Its Controlled Delivery of Cytochrome c via Electrochemical Method[J]. Chinese Journal of Applied Chemistry, 2012, 29(3): 340-345. doi: 10.3724/SP.J.1095.2012.00197
Citation: LIU Qian, QIAN Jing, WANG Kun, LI Yeping, WU Xiangyang. pH Sensitivity of Monomethoxypoly(ethylene glycol)grafted-chitosan and Its Controlled Delivery of Cytochrome c via Electrochemical Method[J]. Chinese Journal of Applied Chemistry, 2012, 29(3): 340-345. doi: 10.3724/SP.J.1095.2012.00197
电化学研究聚乙二醇单甲醚接枝壳聚糖的pH敏感性及对细胞色素c的控制释放
摘要:
采用电化学沉积法在Au电极上制备了聚乙二醇单甲醚接枝壳聚糖(mPEG-g-CS)电沉积膜及其与细胞色素c(Cyt c)复合膜。循环伏安(CV)和交流阻抗(EIS)等电化学手段研究表明,mPEG-g-CS膜具有良好的pH敏感性,通过调节溶液的pH值,可以改变电化学探针[Fe(CN)6]3-/4-在电极表面的电子转移速率;Cyt c在mPEG-g-CS膜内可以实现直接电子转移,该复合膜电极对H2O2的响应快速而灵敏,说明mPEG-g-CS能较好的保持Cyt c的生物活性。通过控制溶液的pH值,实现了Cyt c在mPEG-g-CS膜内的可逆释放和关闭。
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关键词:
- 聚乙二醇单甲醚接枝壳聚糖
- / pH敏感性
- / 细胞色素c
- / 直接电化学
- / 控制释放
English
pH Sensitivity of Monomethoxypoly(ethylene glycol)grafted-chitosan and Its Controlled Delivery of Cytochrome c via Electrochemical Method
Abstract:
A pH-sensitive monomethoxypoly(ethylene glycol)-grafted-chitosan(mPEG-g-CS) film was used as a matrix for reversible immobilization of cytochrome c(Cyt c) on a Au surface through a novel electrodeposition process.The pH-sensitive behavior of the mPEG-g-CS film was demonstrated by cyclic voltammetry(CV) and electrochemical impedance spectroscopy(EIS) measurements.Electrochemical co-deposited Cyt c and mPEG-g-CS exhibited the direct electrochemical behavior which implied that mPEG-g-CS could well control the delivery of Cyt c under different pH.
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