引用本文:
吴会灵, 李文友, 何锡文, 梁宏. 喹哪啶蓝与核酸作用的光谱研究及分析[J]. 应用化学,
2002, 19(7): 672-676.
Citation: WU Hui Ling, LI Wen-You, HE Xi-Wen, LIANG Hong. Spectral Studies on Interactions of Quinaldine Blue with Nucleic Acids and Its Analytical Application[J]. Chinese Journal of Applied Chemistry, 2002, 19(7): 672-676.
Citation: WU Hui Ling, LI Wen-You, HE Xi-Wen, LIANG Hong. Spectral Studies on Interactions of Quinaldine Blue with Nucleic Acids and Its Analytical Application[J]. Chinese Journal of Applied Chemistry, 2002, 19(7): 672-676.
喹哪啶蓝与核酸作用的光谱研究及分析
English
Spectral Studies on Interactions of Quinaldine Blue with Nucleic Acids and Its Analytical Application
Abstract:
In an aqueous solution of pH=5.0~8.2, H aggregates of Quinaldine Blue(QB) are formed with the nucleic acid molecules. QB showed a decrease in absorbance at peak wavelength of 598 nm or 548 nm on binding to nucleic acids. A new assay method for nucleic acids was thus developed based on clear decrease of QB absorbance at 598 nm. The calibration curves are linear in the range of 8.0×10-8~2.0×10-6g/mL for ctDNA, 1.0×10-7~2.0×10-6g/mL for yRNA, respectively. The reliabilities of five determinations of 1.0 μg/mL nucleic acids were 3.4% for ctDNA and 3.6% for yRNA, respectively. The method was featured by its rapidity, simplicity, sensitivity and good selectivity. It has been used to determine both synthetic and real samples with satisfactory results.
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Key words:
- nucleic acid
- / quinaldine blue
- / outside stacking binding
- / spectrophotometry
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