大孔乙酰氨甲基聚苯乙烯吸附树脂的合成及胱氨酸和酪氨酸的分离

引用本文: 刘国栋, 阎虎生, 何炳林, 姜根华, 杨国英. 大孔乙酰氨甲基聚苯乙烯吸附树脂的合成及胱氨酸和酪氨酸的分离[J]. 应用化学, 2002, 19(9): 894-897. shu

Citation: LIU Guo-Dong, YAN Hu-Sheng, HE Bing-Lin, JIANG Gen-Hua, YANG Guo-Ying. Synthesis of Macroporous Acetyl Aminomethylene Polystyrene Resin and Its Application in Separation of Cystine and Tyrosine[J]. Chinese Journal of Applied Chemistry, 2002, 19(9): 894-897. shu

大孔乙酰氨甲基聚苯乙烯吸附树脂的合成及胱氨酸和酪氨酸的分离

刘国栋 ^a, ,
阎虎生 ^{a,
,} ,
何炳林 ^a, ,
姜根华 ^b, ,
杨国英 ^b,

1.
a 吸附与分离功能高分子材料国家重点实验室, 南开大学高分子化学研究所天津 300071;
2.
b 天津药业有限公司天津

通讯作者: 阎虎生,男,42岁,教授,博士生导师;E-mail:yanhs@public.tpt.tj.cn;研究方向:功能高分子和固相多肽合成

基金项目:
天津市重点基金项目(983801411) (983801411)

摘要: 设计合成了能与酪氨酸酚羟基和苯环形成氢键和疏水作用的大孔乙酰氨甲基聚苯乙烯树脂。酪氨酸在填充此树脂的色谱柱上有较大的保留,而胱氨酸基本不保留从而使二者得到分离。通过研究胱氨酸、酪氨酸和苯丙氨酸在大孔乙酰氨甲基聚苯乙烯、大孔聚苯乙烯和聚丙烯酰胺3个树脂柱上的保留,证明酪氨酸和乙酰氨甲基聚苯乙烯树脂之间存在氢键和疏水作用,且二者之间有协同作用,0.4g由蛋白质水解得到的酪氨酸和胱氨酸混合物经大孔乙酰氨甲基聚苯乙烯树脂柱(250×10 mm,I.D.)分离可得到0.20 g纯胱氨酸和0.12 g纯酪氨酸。

关键词:

English

Synthesis of Macroporous Acetyl Aminomethylene Polystyrene Resin and Its Application in Separation of Cystine and Tyrosine

1.
a State Key Laboratory of Functional Polymer Materials for Adsorption and Separation, Institute of Polymer Chemistry, Nankai University, Tianjin 300071;
2.
b Tianjin Pharmaceutical Company, Tianjin

Corresponding author: 阎虎生,男,42岁,教授,博士生导师;E-mail:yanhs@public.tpt.tj.cn;研究方向:功能高分子和固相多肽合成

Received Date: 05 February 2002
Fund Project:
天津市重点基金项目(983801411)

Abstract: Based on the fact that tyrosine molecule contains a phenyl ring and a phenolic hydroxyl group, a polymeric adsorbent-macroporous acetyl aminomethylene polystyrene resin, which was expected to form hydrogen bonding and hydrophobic interaction with tyrosine but not with cystine, was synthesized. The results indicated that tyrosine had high retention on a column packed with the adsorbent while cystine had no retention. By comparing the retentions of cystine, tyrosine and phenylalanine on columns packed with macroporous acetyl aminomethylene polystyrene resin, macro porous polystyrene resin and polyacrylamide resin, respectively, it was concluded that there are hydrophobic interaction and hydrogen bonding between tyrosine and macroporous acetyl aminomethylene polystyrene resin and the two interactions acted synergistically. From 0.4 g mixture of cystine and tyrosine, an industrial byproduct of amino acids from hydrolysis of proteins on a column(250 mm×10 mm, I.D.) packed with macroporous acetyl aminomethylene polystyrene resin 0.2 g of pure cystine and 0.12 g of pure tyrosine were obtained, respectively.

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大孔乙酰氨甲基聚苯乙烯吸附树脂的合成及胱氨酸和酪氨酸的分离

通讯作者: 阎虎生,男,42岁,教授,博士生导师;E-mail:yanhs@public.tpt.tj.cn;研究方向:功能高分子和固相多肽合成

English

Synthesis of Macroporous Acetyl Aminomethylene Polystyrene Resin and Its Application in Separation of Cystine and Tyrosine

Corresponding author: 阎虎生,男,42岁,教授,博士生导师;E-mail:yanhs@public.tpt.tj.cn;研究方向:功能高分子和固相多肽合成

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

大孔乙酰氨甲基聚苯乙烯吸附树脂的合成及胱氨酸和酪氨酸的分离

通讯作者: 阎虎生,男,42岁,教授,博士生导师;E-mail:yanhs@public.tpt.tj.cn;研究方向:功能高分子和固相多肽合成

English

Synthesis of Macroporous Acetyl Aminomethylene Polystyrene Resin and Its Application in Separation of Cystine and Tyrosine

Corresponding author: 阎虎生,男,42岁,教授,博士生导师;E-mail:yanhs@public.tpt.tj.cn;研究方向:功能高分子和固相多肽合成

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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