引用本文:
袁春桃, 蒋先明. 壳聚糖-g-丙烯腈固定化木瓜蛋白酶的研究[J]. 应用化学,
2002, 19(9): 862-865.
Citation: YUAN Chun-Tao, IANG Xian-Ming. Immobilization of Papain onto Poly(chitosan-co-acrylonitrile)[J]. Chinese Journal of Applied Chemistry, 2002, 19(9): 862-865.
Citation: YUAN Chun-Tao, IANG Xian-Ming. Immobilization of Papain onto Poly(chitosan-co-acrylonitrile)[J]. Chinese Journal of Applied Chemistry, 2002, 19(9): 862-865.
壳聚糖-g-丙烯腈固定化木瓜蛋白酶的研究
摘要:
以接枝共聚物壳聚糖-g-丙烯腈作为载体固定化木瓜蛋白酶,考察了pH值、温度、离子强度和给酶量对固定化木瓜蛋白酶活力的影响,研究了固定化酶的性质,如米氏常数、热稳定性和操作半衰期,并与壳聚糖固定化木瓜蛋白酶、溶液酶进行了比较。所制得的壳聚糖-g-丙烯腈固定化木瓜蛋白酶的最适宜的温度为70℃。70℃下9h后固定化酶的活力仍可保持91%。实验表明,壳聚糖-g-丙烯腈可以较好地吸附、包结木瓜蛋白酶,固定化酶的酶活力较高,且稳定性得到提高。用固定化酶处理啤酒,低温冷藏(4℃)70 d后,无冷浑浊现象出现,浊度仅为原来的1.05倍,仍可保持啤酒原有的风味和理化性质。
English
Immobilization of Papain onto Poly(chitosan-co-acrylonitrile)
Abstract:
Papain was immobilized on poly(chitosan-co-acrylonitrile). The properties of immobilized enzyme such as Michaels constant, thermal stability and half-working life were studied. The results showed that the matrix could greatly adsorb papain with thermal stability increased. The optimum immobilization temperature was 70℃. After treatment at 70℃ for 9 h, 91% activity of the immobilized papain was retained. In comparison with native enzyme the thermal stability of immobilized papain on poly(chitosan-co-acrylonitrile) was improved considerably. After at storage 4℃ for 70 d, no turbidness was observed in beer treated with poly(chitosan-co-acrylonitrile) immobilized papain.
-
Key words:
- chitosan
- / graft copolymer
- / immobilized
- / papain
- / beer
-
-
扫一扫看文章
计量
- PDF下载量: 1
- 文章访问数: 492
- HTML全文浏览量: 60
下载:
