Abstract: Resveratrol,a promising anti-cancer agent,is an important active ingredent in Polygonum cuspidatum Sieb.et Zucc.A molecular imprinted polymer was prepared by virtue of molecular imprinting technique,with trans-resveratrol as the template molecular,acrylamide as the functional monomer,EGDMA as the cross-linking agent.The molecular imprinted polymer was investigated with the aid of equilibrium binding experiments to evaluate the molecular recognition and binding characteristics of the trans-resveratrol molecularly imprinted polymer.The scatchard analysis showed that two kinds of binding sites were produced in the polymer matrix and their dissociation constants were calculated to be 9.9×10^-5 mol/L,1.7×10^-3 mol/L,respec-tively.The results show that compared with non-imprinted polymer,the imprinted polymer exhibits a much higher affinity for trans-resveratrol among the tested compounds Res,Pol and BPA.Also,the imprinted polymer was applied to separate resveratrol from Polygonum cuspidatum Sieb.et Zucc.extracts and good results were obtained.