引用本文:
冯玉英, 吴晓红, 周家宏, 顾晓天, 陆天虹, 王雪松, 张宝文. 竹红菌甲素与血红蛋白相互作用光谱[J]. 应用化学,
2005, 22(8): 895-898.
Citation: FENG Yu-Ying, WU Xiao-Hong, ZHOU Jia-Hong, GU Xiao-Tian, LU Tian-Hong, WANG Xue-Song, ZHANG Bao-Wen. Study on Interaction Between Hypocrellin A and Hemoglobin Using Spectral Methods[J]. Chinese Journal of Applied Chemistry, 2005, 22(8): 895-898.
Citation: FENG Yu-Ying, WU Xiao-Hong, ZHOU Jia-Hong, GU Xiao-Tian, LU Tian-Hong, WANG Xue-Song, ZHANG Bao-Wen. Study on Interaction Between Hypocrellin A and Hemoglobin Using Spectral Methods[J]. Chinese Journal of Applied Chemistry, 2005, 22(8): 895-898.
竹红菌甲素与血红蛋白相互作用光谱
English
Study on Interaction Between Hypocrellin A and Hemoglobin Using Spectral Methods
Abstract:
The interaction of horse heart hemoglobin with Hypocrellin A (HA) was studied by means of UV-Vis absorption spectroscopy and fluorescence spectroscropy under physiological condition.In the UV-Vis absorption spectrum,the intensity of the absorption peak of amino acid residues in the hemoglobin molecule decreases and the peak shifts bathochromically due to the hydrogen bonding between the amino acid residue in the hemoglobin molecule and HA.The synchronous fluorescence spectra show that the degree of the interaction of HA with different amino acid residues are different.For example,the fluorescence quenching kinetic constants of HA for tryptophane and tyrosine residues were 5.5×1012 and 1.7×1012 L/mol,respectively,which clearly demonstrates that the interaction between HA and tryptophane residue is more intense than that between HA and tyrosine residue.
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