Abstract: Distribution behavior of terbutaline enantiomorph was investigated in a aqueous/organic two-phase system containing a chiral selector (L-dihexyltartrate).The influences of organic solvents,lipophilic anions and pH value on the distribution coefficient (k) and the separation factor (α) were investigated.The results show that tetraphenylborate lipophilic anion and terbutaline enantiomorph form two lipophilic salt complexes,which facilitates the solubilization of the enantiomorph in the organic phase.The extraction performance of the two kinds of organic solvent is different,and n-heptane is a better organic solvent.However,pH value of the aqueous phase also influences the extraction performance obviously.Under the determined extraction conditions,a new process of countercurrent liquid membrane extraction technology was adopted to separate terbutaline enantiomorph.To demonstrate the feasibility of the system,mass transfer experiments were performed,in which terbutaline enantiomorph of 99% optical purity can be obtained.