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Studies of the intestinal absorption of the alkaloids in the Wu-tou decoction combined with different incompatible medicinal herbs in a Caco-2 cell culture system using UPLC-MS/MS

Hong Kan ,  Wen-Yue Jiang ,  Ru Ding ,  Zhong-Ying Liu ,  Zi-Feng Pi ,  Zhi-Qiang Liu

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Citation:  Hong Kan, Wen-Yue Jiang, Ru Ding, Zhong-Ying Liu, Zi-Feng Pi, Zhi-Qiang Liu. Studies of the intestinal absorption of the alkaloids in the Wu-tou decoction combined with different incompatible medicinal herbs in a Caco-2 cell culture system using UPLC-MS/MS[J]. Chinese Chemical Letters, 2015, 26(5): 590-594. doi: 10.1016/j.cclet.2014.12.008 shu

Studies of the intestinal absorption of the alkaloids in the Wu-tou decoction combined with different incompatible medicinal herbs in a Caco-2 cell culture system using UPLC-MS/MS

    • 通讯作者: Zhong-Ying Liu,
    Zi-Feng Pi,
  • 基金项目:

    This work was supported by the National Natural Sciences Foundation of China (No. 81274046)  (No. 81274046)

    National Basic Research Program of China ("973 Program") (Nos. 2011CB505300, 2011CB505305). ("973 Program")

摘要: In this study, seven alkaloids were detected in Wu-tou decoction using ultra performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MSn). The aim of this study was to investigate the effect of Fritillariae Cirrhosae Bulbus, Fritillariae Thunbergii Bulbus, Pinelliae Rhizoma in different ratios with Wu-tou decoction (2:1, 1:1, 1:2) by measuring the therapeutic effects in Wu-tou decoction of main seven alkaloids including benzoylaconitine (BA), benzoylmesaconitine (BM), benzoylhypaconitine (BH), hypaconitine (HA), fuziline (FU), niaolin (NE) and deoxyaconitine (DA). The permeability of aconitum alkaloids extract through a Caco-2 cell monolayer was analyzed in the absence and presence of Fritillariae Cirrhosae Bulbus, Fritillariae Thunbergii Bulbus, and Pinelliae Rhizoma, respectively. The results showed that Pinelliae Rhizoma could reduce the absorption of the alkaloids and increase the excretion of the alkaloids, which would attenuate the therapeutic effects of Wu-tou decoction. Therefore, Pinelliae Rhizoma is an incompatible herb of Wu-tou decoction because of the inhibition of the absorption of alkaloids in the intestine. And that Fritillariae Cirrhosae Bulbus and Fritillariae Thunbergii Bulbus showed the effects to improve the permeability of the alkaloids in Wu-tou decoction. These effects of these two herbs were similar, but the former was stronger than the latter, which most likely is due to the fact that the compositions of these two traditional Chinese medicines are similar. The in vitro data suggests that the compounds such as fritillary presented in alkaloids in the formula maybe improve the therapeutic function caused by the increased bioavailability of alkaloids in intestine.

English

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  • 发布日期:  2014-12-16
  • 收稿日期:  2014-09-10
  • 网络出版日期:  2014-12-01
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