罗丹明内酰胺和铜离子荧光法检测人工湿地中的磷酸根离子

引用本文: 杨慧, 郑林玲, 李原芳, 黄承志. 罗丹明内酰胺和铜离子荧光法检测人工湿地中的磷酸根离子[J]. 分析化学, 2012, 40(11): 1715-1719. doi: 10.3724/SP.J.1096.2012.20110 shu

Citation: YANG Hui, ZHENG Lin-Ling, LI Yuan-Fang, HUANG Cheng-Zhi. Rhodamine Spirolactam and Copper Ion-based Fluorometric Detection of Phosphate in Artificial Wetlands[J]. Chinese Journal of Analytical Chemistry, 2012, 40(11): 1715-1719. doi: 10.3724/SP.J.1096.2012.20110 shu

罗丹明内酰胺和铜离子荧光法检测人工湿地中的磷酸根离子

杨慧 ^1, ,
郑林玲 ^1, ,
李原芳 ^1, ,
黄承志 ^{1,2,
,}

通讯作者: 黄承志

基金项目:
本文系国家自然科学基金(No. 21035005)资助 (No. 21035005)

摘要: 研究发现, PO₄^3-在中性条件下抑制Cu²⁺催化罗丹明内酰胺水解生成强荧光罗丹明B的反应,抑制的程度与PO₄^3-的浓度具有线性关系。由此,建立了检测PO₄^3-的荧光分光光度法。在pH 7.0的条件下,方法的线性范围为3.0×10^-6-4.5×10^-5 mol/L,相关系数为0.9938,检出限(3σ)为1.6×10^-7 mol/L。本方法用于人工湿地中PO₄^3-的检测,加标回收率为93.0%-108.0%,相对标准偏差小于6.0%。

关键词:

English

Rhodamine Spirolactam and Copper Ion-based Fluorometric Detection of Phosphate in Artificial Wetlands

1.
¹ Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemical and Chemistry Engineering, Southwest University, Chongqing 400715, China;

Corresponding author: 黄承志

Received Date: 10 February 2012
Fund Project:
本文系国家自然科学基金(No. 21035005)资助

Abstract: Under neutral condition, phosphate ions could inhibit the catalytic effect of copper ions on the hydrolysis of rhodamine spirolactam to form fluorescent rhodamine B, resulting in the decrease of fluorescent intensity. Moreover, the inhibitive degree was in proportional to the concentration of phosphate ions, which could be developed for sensing phosphate. In the buffer (pH 7.0) solution, phosphate ions could be determined in the range of 3.0×10^-6-4.5×10^-5mol/L with the correlation coefficient of 0.9938 and the detection limit of 1.6×10^-7 mol/L (3σ). This method was applied to the detection of phosphate in artificial wetlands with the recovery from 93.0% to 108.0% and the RSD lower than 6.0%.

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

罗丹明内酰胺和铜离子荧光法检测人工湿地中的磷酸根离子

通讯作者: 黄承志

English

Rhodamine Spirolactam and Copper Ion-based Fluorometric Detection of Phosphate in Artificial Wetlands

Corresponding author: 黄承志

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

罗丹明内酰胺和铜离子荧光法检测人工湿地中的磷酸根离子

通讯作者: 黄承志

English

Rhodamine Spirolactam and Copper Ion-based Fluorometric Detection of Phosphate in Artificial Wetlands

Corresponding author: 黄承志

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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