石墨烯/导电高分子/离子液体修饰的黄曲霉毒素B<sub>1</sub>免疫传感器的制备及应用

引用本文: 周琳婷, 李在均, 方银军. 石墨烯/导电高分子/离子液体修饰的黄曲霉毒素B₁免疫传感器的制备及应用[J]. 分析化学, 2012, 40(11): 1635-1641. doi: 10.3724/SP.J.1096.2012.20151 shu

Citation: ZHOU Lin-Ting, LI Zai-Jun, FANG Yin-Jun. Preparation and Application of Graphene/Conductive Polymer/Ionic Liquid Immunosensor for Determination of Aflatoxin B₁[J]. Chinese Journal of Analytical Chemistry, 2012, 40(11): 1635-1641. doi: 10.3724/SP.J.1096.2012.20151 shu

石墨烯/导电高分子/离子液体修饰的黄曲霉毒素B₁免疫传感器的制备及应用

周琳婷 ^1, ,
李在均 ^{1,
,} ,
方银军 ^2,

通讯作者: 李在均

基金项目:
本文系国家科技支撑计划(No.2011BAK10B032) (No.2011BAK10B032)

国家自然科学基金(No.21176101) (No.21176101)

浙江省自然科学基金(No.Y4100729) (No.Y4100729)

江苏省"青蓝工程"资助项目

摘要: 依次电沉积氧化石墨烯、2,5-二(2-噻吩)-1-对苯甲酸吡咯和氯金酸于金电极表面, 以EDC/NHS为活化剂, 将黄曲霉毒素B₁(AFB₁)抗体共价连接在导电高分子膜上, 最后滴涂1,3-二丁基咪唑六氟磷酸盐离子液体于上述修饰电极表面, 制得AFB₁免疫传感器。以Fe(CN)₆^3-/4-的磷酸盐缓冲溶液(pH 7.0)为测试底液, 采用循环伏安法和交流阻抗法考察此免疫传感器的电化学行为。研究表明: 石墨烯和纳米金的引入明显提高了修饰层的电子转移速率, 使电极的表观活性面积由裸金电极的0.1772 cm²增加到0.2188 cm²和0.2640 cm²。当AFB₁浓度在3.2×10^-15-3.2×10^-13 mol/L范围内, 传感器的交流阻抗响应值与浓度呈线性关系, 相关系数R²=0.994, 检出限为1.1×10^-15 mol/L。传感器在4℃下保存20周以上, 电化学响应保持基本不变。本方法的灵敏度和稳定性优于现有文献报道, 并应用于花生样品中痕量AFB₁的测定。

关键词:

English

Preparation and Application of Graphene/Conductive Polymer/Ionic Liquid Immunosensor for Determination of Aflatoxin B₁

1.
¹ School of Chemical and Materials Engineering, Jiangnan University, Wuxi 214122, China;

Corresponding author: 李在均

Received Date: 21 February 2012
Fund Project:
本文系国家科技支撑计划(No.2011BAK10B032)

国家自然科学基金(No.21176101)

浙江省自然科学基金(No.Y4100729)

江苏省"青蓝工程"资助项目

Abstract: To fabricate the aflatoxin B₁ immunosensor, graphene oxide, 2,5-di-(2-thienyl)-1-pyrrole-1-(p-benzoic acid) and chloroauric acid were electrodeposited on the gold electrode surface in sequence. Then aflatoxin B₁ (AFB₁) antibody was covalently connected to the conducting polymer film with 1-ethyl-(3-dimethyl-aminopropyl)-carbodimide/N-hydroxysuccimimide (EHC/NHS) as activator and 1,3-dibutyliminazolium hexafluorophosphate ionic liquid was finally coated on the modified electrode. The Fe(CN)₆^3-/4- phosphate buffer solution (pH 7.0) was employed as base solution for investigating electrochemical performances of the immunosensor by cyclic voltammetry and electrochemical impedance spectroscopy. Research revealed the introduction of graphene and gold nanocomposite obviously improved electron transfer rate of the modified layer, and the apparent electroactive surface areas of the electrode also increased up to 0.2188 cm² and 0.2640 cm² from 0.1772 cm² of bare gold electrode, respectively. When aflatoxin B₁ concentration is in the range of 3.2×10^-15-3.2×10^-13 mol/L, the electron transfer impedance responses of the sensor will linearly increase. The correlation coefficient (R²) and the detection limit were found to be 0.994 and 1.1×10^-15 mol/L. The electrochemical response of the immunosenor can keep almost constant after stored at 4℃ for 20 weeks. Sensitivity and stability of the proposed method are better than those of other methods reported in literatures, it has been successfully applied to determination of trace AFB₁ in peanut samples.

Key words:

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1.
沈阳化工大学材料科学与工程学院沈阳 110142

石墨烯/导电高分子/离子液体修饰的黄曲霉毒素B₁免疫传感器的制备及应用

通讯作者: 李在均

English

Preparation and Application of Graphene/Conductive Polymer/Ionic Liquid Immunosensor for Determination of Aflatoxin B₁

Corresponding author: 李在均

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

石墨烯/导电高分子/离子液体修饰的黄曲霉毒素B1免疫传感器的制备及应用

通讯作者: 李在均

English

Preparation and Application of Graphene/Conductive Polymer/Ionic Liquid Immunosensor for Determination of Aflatoxin B1

Corresponding author: 李在均

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

石墨烯/导电高分子/离子液体修饰的黄曲霉毒素B₁免疫传感器的制备及应用

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