气相色谱-质谱法测定植物油中8种维生素E及其在芝麻油真伪鉴别方面的应用

引用本文: 沈伟健, 王红, 陆慧媛, 余可垚, 胡国绅, 魏雪缘, 吴斌. 气相色谱-质谱法测定植物油中8种维生素E及其在芝麻油真伪鉴别方面的应用[J]. 色谱, 2020, 38(5): 595-599. doi: 10.3724/SP.J.1123.2019.09011 shu

Citation: SHEN Weijian, WANG Hong, LU Huiyuan, YU Keyao, HU Guoshen, WEI Xueyuan, WU Bin. Determination of eight vitamin E in vegetable oils by gas chromatography-mass spectrometry and its application on authentication of sesame oil[J]. Chinese Journal of Chromatography, 2020, 38(5): 595-599. doi: 10.3724/SP.J.1123.2019.09011 shu

气相色谱-质谱法测定植物油中8种维生素E及其在芝麻油真伪鉴别方面的应用

南京海关动植物与食品检测中心, 江苏南京 210019

通讯作者: 沈伟健, E-mail:45467447@qq.com

基金项目:
南京海关科研计划项目（2018KJ38）.

摘要: 建立了气相色谱-质谱（GC-MS）同时测定植物油中α-、β-、γ-、δ-生育酚和α-、β-、γ-、δ-生育三烯酚等8种维生素E的分析方法。植物油样品经甲醇超声提取、浓缩、定容，在分时段选择离子监测（SIM）模式下分离分析，采用外标法进行定量。结果表明，8种维生素E可实现基线分离；在0.01~1 mg/L范围内，所有目标物均呈良好线性关系，相关系数均大于0.99；检出限和定量限分别为0.03~0.25 mg/kg和0.10~0.83 mg/kg；在芝麻油中分别添加10、50和250 mg/kg 3个水平的8种维生素E进行加标试验，平均回收率为87.5%~107.4%，相对标准偏差（RSD）≤ 7.5%。所建立的方法简单、准确、可靠，且灵敏度高，可用于测定植物油中8种维生素E的含量。采用上述方法对芝麻油、大豆油、菜籽油、葵花籽油、花生油、玉米油和棕榈油等7种共75个植物油样品中维生素E的含量进行测定。结果显示，芝麻油与其他6种植物油中的8种维生素E的组成和含量均有显著差异性，因此该方法可作为芝麻油掺入其他植物油的特征鉴定指标。

关键词:

English

Determination of eight vitamin E in vegetable oils by gas chromatography-mass spectrometry and its application on authentication of sesame oil

Animal, Plant and Food Inspection Center, Nanjing Customs, Nanjing 210019, China

Corresponding author: SHEN Weijian, 45467447@qq.com

Received Date: 09 September 2019
Fund Project:
Nanjing Customs Scientific Research Project (No. 2018KJ38).

Abstract: A method was established for the determination of eight vitamins E (α-, β-, γ-, δ-tocopherol and α-, β-, γ-, δ-tocotrienol) in vegetable oils using gas chromatography-mass spectrometry (GC-MS). The targets were extracted with methanol, and analyzed by GC-MS in the selected ion monitoring (SIM) mode after concentration to a constant volume, and quantified using the external standard method. Baseline separation were achieved for all the target compounds. The linearities of all the compounds were between 0.01 and 1 mg/L. The limits of detection (LODs) and limits of quantification (LOQs) were in the range of 0.03-0.25 mg/kg and 0.10-0.83 mg/kg, respectively. The average recoveries of all the targets in sesame oil samples were between 87.5% and 107.4% at three spiked levels (10, 50, and 250 mg/kg), and the RSDs were all less than 7.5%. The tocopherols and tocotrienols contents in sesame oil samples and in six lower-price vegetable oils (soybean, rapeseed, sunflower, peanut, corn and palm oils) were determined by the above mentioned method. The results showed that the vitamin E profiles of sesame oil were significantly different from those of the other six vegetable oils. Therefore, vitamin E can be used as a discriminating parameter for detecting the adulteration of sesame.

Key words:

gas chromatography-mass spectrometry (GC-MS)
/ tocopherol
/ tocotrienol
/ vitamin E
/ sesame oil
/ authentication

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气相色谱-质谱法测定植物油中8种维生素E及其在芝麻油真伪鉴别方面的应用

通讯作者: 沈伟健, E-mail:45467447@qq.com

English

Determination of eight vitamin E in vegetable oils by gas chromatography-mass spectrometry and its application on authentication of sesame oil

Corresponding author: SHEN Weijian, 45467447@qq.com

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

气相色谱-质谱法测定植物油中8种维生素E及其在芝麻油真伪鉴别方面的应用

通讯作者: 沈伟健, E-mail:45467447@qq.com

English

Determination of eight vitamin E in vegetable oils by gas chromatography-mass spectrometry and its application on authentication of sesame oil

Corresponding author: SHEN Weijian, 45467447@qq.com

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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