固相萃取-超高效液相色谱法测定人参中5种原人参二醇型人参皂苷的含量

引用本文: 宋春颖, 张华蓉, 郭志谋, 闫竞宇, 金高娃, 梁鑫淼. 固相萃取-超高效液相色谱法测定人参中5种原人参二醇型人参皂苷的含量[J]. 色谱, 2020, 38(5): 547-553. doi: 10.3724/SP.J.1123.2019.09003 shu

Citation: SONG Chunying, ZHANG Huarong, GUO Zhimou, YAN Jingyu, JIN Gaowa, LIANG Xinmiao. Determination of five protopanaxadiol ginsenosides in ginseng by solid-phase extraction-ultra performance liquid chromatography[J]. Chinese Journal of Chromatography, 2020, 38(5): 547-553. doi: 10.3724/SP.J.1123.2019.09003 shu

固相萃取-超高效液相色谱法测定人参中5种原人参二醇型人参皂苷的含量

1.
中国科学院大连化学物理研究所, 中国科学院分离分析化学重点实验室, 辽宁大连 166023;
2.
中国科学院大学, 北京 100049;
3.
中科院大化所中国医药城生物医药创新研究院, 江苏泰州 225300

通讯作者: 金高娃, E-mail:jingw@dicp.ac.cn

基金项目:
国家自然科学基金重点基金（U1508221）.

摘要: 建立了固相萃取-超高效液相色谱测定人参中5种原人参二醇型人参皂苷的方法。人参药材经粉碎后通过水饱和正丁醇溶液进行超声提取，经过亲水作用固相萃取柱净化后，在ACQUITY UPLC BEH Shield RP18色谱柱（100 mm×2.1 mm，1.7 μm）上分离，利用乙腈/水作为流动相进行梯度洗脱，采用光电二极管阵列检测器检测。结果表明，5种原人参二醇型人参皂苷在5~500 μg/mL范围内具有很好的线性关系，相关系数均大于0.999。方法精密度的RSD值在0.95%~2.62%（n=6）之间，22 h内样品稳定性的RSD值在0.90%~2.15%（n=8）之间，日内和日间重复性的RSD值分别为5.35%~6.47%（n=6）和5.56%~6.34%（n=8）。方法的加标回收率在87.16%~101.92%之间，相对标准偏差在1.54%~4.01%（n=6）之间。所建立的方法采用亲水作用色谱模式的固相萃取材料，药材的提取液可直接作为固相萃取的上样溶液进行人参皂苷的富集和净化，并且超高效液相色谱大大缩短了分析时间。该方法简单快速、通量高、重现性好，适用于人参中5种原人参二醇型人参皂苷的定量分析。

关键词:

English

Determination of five protopanaxadiol ginsenosides in ginseng by solid-phase extraction-ultra performance liquid chromatography

1.
Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;
2.
University of Chinese Academy of Sciences, Beijing 100049, China;
3.
DICP-CMC Innovation Institute of Medicine, Taizhou 225300, China

Corresponding author: JIN Gaowa, jingw@dicp.ac.cn

Received Date: 02 September 2019
Fund Project:
the State Key Program of National Natural Science of China (No. U1508221).

Abstract: A new method based on solid-phase extraction-ultraperformance liquid chromatography (SPE-UPLC) was developed for the determination of five protopanaxadiol ginsenosides in ginseng. The ginsenosides were extracted from ground ginseng samples using water-saturated n-butanol and purified on a hydrophilic solid-phase extraction column. Chromatographic separation was achieved on an ACQUITY UPLC BEH Shield RP18 column (100 mm×2.1 mm, 1.7 μm) by linear gradient elution using an acetonitrile/water mobile phase. Five protopanaxadiol ginsenosides were detected by a photodiode array detector, and they showed a strong positive linear correlation (r²>0.999) in the range of 5-500 μg/mL. In addition, the instrument precision ranged between 0.95% and 2.62% (n=6), with the sample stability between 0.90% and 2.15% (n=8) within 22 h. Intra- and inter-day repeatabilities were 5.35%-6.47% (n=6) and 5.56%-6.34% (n=8), respectively. Sample recoveries and the corresponding relative standard deviations (RSDs) were 87.16%-101.92% and 1.54%-4.01% (n=6), respectively. Hydrophilic chromatography materials were used in SPE, and the extract was directly loaded and purified without pretreatment. Besides, with the use of UPLC, the analysis time was greatly shortened. The developed method is simple and rapid, with high throughput, thus being suitable for the quantitative analysis of the five protopanaxadiol ginsenosides in ginsengs.

Key words:

hydrophilic interaction chromatography (HILIC)
/ solid-phase extraction (SPE)
/ ultraperformance liquid chromatography (UPLC)
/ ginsenoside
/ ginseng

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沈阳化工大学材料科学与工程学院沈阳 110142

固相萃取-超高效液相色谱法测定人参中5种原人参二醇型人参皂苷的含量

通讯作者: 金高娃, E-mail:jingw@dicp.ac.cn

English

Determination of five protopanaxadiol ginsenosides in ginseng by solid-phase extraction-ultra performance liquid chromatography

Corresponding author: JIN Gaowa, jingw@dicp.ac.cn

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

固相萃取-超高效液相色谱法测定人参中5种原人参二醇型人参皂苷的含量

通讯作者: 金高娃, E-mail:jingw@dicp.ac.cn

English

Determination of five protopanaxadiol ginsenosides in ginseng by solid-phase extraction-ultra performance liquid chromatography

Corresponding author: JIN Gaowa, jingw@dicp.ac.cn

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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